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1.发明申请In-Vivo Non-Viral Gene Delivery of Human Vascular Endothelial Growth Factor Following Islet Transplantation 审中-公开体外非病毒基因递送人类血管内皮生长因子胰岛移植后公开(公告)号：US20110182979A1
公开(公告)日：2011-07-28
申请号：US13014926
申请日：2011-01-27
CPC分类号： A61K35/39 , A61K9/2077 , A61K38/1866 , A61K48/0075 , C12N15/87 , A61K2300/00
摘要： An ultrasound-mediated gene transfer method named Ultrasound Targeted Microbubble Destruction (UTMD) for the delivery of human vascular endothelial growth factor (hVEGF) gene to transplanted islets and the surrounding tissue is described herein. The delivery of hVEGF promotes islet revascularization and survival. The inventors, first transplanted human islets were transplanted into diabetic nude mice liver followed by the induction of non-viral plasmid vectors encoding hVEGF or Green Fluorescent Protein (GFP) gene in the host liver by UTMD. Transplantation without gene delivery was also performed as a control. Blood glucose, serum human insulin, C-peptide levels and the revascularization in graft islets were evaluated. The findings of the method of the present invention indicated that hVEGF gene delivery to host liver using UTMD promoted islet revascularization after islet transplantation and improved the restoration of euglycemia.
摘要翻译： 本文描述了称为超声靶向微泡破坏（UTMD）的超声介导的基因转移方法，用于将移植的胰岛和周围组织递送人血管内皮生长因子（hVEGF）基因。 hVEGF的输送促进胰岛血运重建和存活。 将本发明人首先将移植的人胰岛移植到糖尿病裸小鼠肝脏中，然后通过UTMD诱导宿主肝脏中编码hVEGF或绿色荧光蛋白（GFP）基因的非病毒质粒载体。 也进行无基因递送移植作为对照。 评估血糖，血清人胰岛素，C肽水平和移植胰岛血运重建。 本发明方法的发现表明，使用UTMD的hVEGF基因递送至宿主肝，促进了胰岛移植后胰岛血运重建，改善了正常血糖的恢复。
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2.发明申请NEUROD1 GENE EXPRESSION IN NON-ENDOCRINE PANCREATIC EPITHELIAL CELLS (NEPECs) 审中-公开非内皮素胰腺上皮细胞中的NEUROD1基因表达（NEPECs）公开(公告)号：US20120009244A1
公开(公告)日：2012-01-12
申请号：US13071458
申请日：2011-03-24
CPC分类号： C12N15/85 , A61K38/1883 , C07K14/4702 , C12N2830/008
摘要： The introduction of the human NeuroD1 gene into human non-endocrine pancreatic epithelial cells (NEPECs) for producing insulin producing cells in vitro is described herein. Cytokeratin19 (CK19) positive NEPECs were transfected with plasmids encoding human NeuroD1 gene under human CK19 promoter. On characterization following the induction it was found that NEPEC+ND strongly expressed NeuroD1 and insulin mRNA. The ratio of NeuroD1 and human insulin positive cells in NEPEC+ND was significantly higher than NEPEC. Human insulin and C-peptide levels in culture media in NEPEC+ND were significantly higher than NEPEC. The findings demonstrate that human NeuroD1 under control of the CK19 promoter induces the differentiation of CK19 positive NEPECs into insulin producing cells.
摘要翻译： 本文描述了将人NeuroD1基因导入用于在体外产生胰岛素产生细胞的人非内分泌胰腺上皮细胞（NEPEC）中。 在人CK19启动子下用编码人NeuroD1基因的质粒转染细胞角蛋白19（CK19）阳性NEPEC。 在诱导后的表征中，发现NEPEC + ND强烈表达NeuroD1和胰岛素mRNA。 NEPEC + ND中NeuroD1和人胰岛素阳性细胞的比例显着高于NEPEC。 NEPEC + ND培养基中人胰岛素和C肽水平明显高于NEPEC。 研究结果表明，CK19启动子控制下的人NeuroD1诱导CK19阳性NEPEC分化为胰岛素生成细胞。
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公开(公告)号：US20100166718A1
公开(公告)日：2010-07-01
申请号：US12645910
申请日：2009-12-23
CPC分类号： A61K35/39 , C12N5/0676
摘要： The present invention includes a method of isolating pancreatic islets by density centrifugation wherein the pancreatic islets are loaded in a solution comprising pancreatic islets with the density gradient and the islets are isolated by centrifuging the vessel wherein the pressure on the pancreatic islets is less than 50 Pa and wherein the pancreatic islets are isolated from the gradient, the improvement comprising creating a continuous density gradient in a vessel comprising at least 100 milliliters with a bend tube that reduces convection currents that disrupt the gradient.
摘要翻译： 本发明包括通过密度离心分离胰岛的方法，其中将胰岛装载在包含具有密度梯度的胰岛的溶液中，通过离心分离胰岛，其中胰岛上的压力小于50Pa 并且其中所述胰岛从所述梯度中分离，所述改进包括在容器中产生包括至少100毫升的连续密度梯度，所述弯管用于减少破坏所述梯度的对流。
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公开(公告)号：US20080110148A1
公开(公告)日：2008-05-15
申请号：US11895865
申请日：2007-08-28
申请人： Kazuo Itoh , Mitsuo Inoue , Masayuki Shimoda
发明人： Kazuo Itoh , Mitsuo Inoue , Masayuki Shimoda
IPC分类号： A01D75/18
CPC分类号： A01D34/90
摘要： A power-driven grass mower includes an operation division having a grass cutter; a motor division having an electric motor and a power-source division having a battery, wherein the operation division is provided with a first means for watching if the motor division is locked for some reason or other, and a second means for putting the motor division into the inching operation in response to any signal from the first means indicating that the motor division is in a locking condition.
摘要翻译： 动力驱动的割草机包括具有割草机的操作部分; 具有电动机的电机部分和具有电池的电源部分，其中所述操作部分设置有用于由于某种原因等待所述电动机分配是否被锁定的第一装置，以及用于将所述电动机分配 响应于来自第一装置的任何指示电动机分配处于锁定状态的信号进入微动作业。
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公开(公告)号：US07886509B2
公开(公告)日：2011-02-15
申请号：US11895865
申请日：2007-08-28
申请人： Kazuo Itoh , Mitsuo Inoue , Masayuki Shimoda
发明人： Kazuo Itoh , Mitsuo Inoue , Masayuki Shimoda
IPC分类号： A01D41/14
CPC分类号： A01D34/90
摘要： A power-driven grass mower includes an operation division having a grass cutter; a motor division having an electric motor and a power-source division having a battery, wherein the operation division is provided with a first means for watching if the motor division is locked for some reason or other, and a second means for putting the motor division into the inching operation in response to any signal from the first means indicating that the motor division is in a locking condition.
摘要翻译： 动力驱动的割草机包括具有割草机的操作部分; 具有电动机的电机部分和具有电池的电源部分，其中所述操作部分设置有用于由于某种原因等待所述电动机分配是否被锁定的第一装置，以及用于将所述电动机分配 响应于来自第一装置的任何指示电动机分配处于锁定状态的信号进入微动作业。
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