公开(公告)号：US20070231901A1

公开(公告)日：2007-10-04

申请号：US11607388

申请日：2006-12-01

申请人： Shuichi Takayama ,  Nobuyuki Futai ,  Yun Heo ,  Gary Smith ,  Jonathan Song

发明人： Shuichi Takayama ,  Nobuyuki Futai ,  Yun Heo ,  Gary Smith ,  Jonathan Song

IPC分类号： C12N1/20 ,  C12N5/00

CPC分类号： C12N1/38 ,  B01L3/5027 ,  C12M23/16 ,  C12M23/34

摘要： A microfluidic cell culture media may have an initial concentration of an active material less than a therapeutic window of concentration necessary to provide a cellular mass a therapeutic environment.

摘要翻译： 微流体细胞培养基可以具有小于提供细胞群体治疗环境所必需的浓度的治疗窗口的活性物质的初始浓度。

公开(公告)号：US20070084706A1

公开(公告)日：2007-04-19

申请号：US11582035

申请日：2006-10-17

申请人： Shuichi Takayama ,  Lourdes Cabrera ,  Nobuyuki Futai ,  Yun Heo ,  Gary Smith

发明人： Shuichi Takayama ,  Lourdes Cabrera ,  Nobuyuki Futai ,  Yun Heo ,  Gary Smith

IPC分类号： H01H29/00

CPC分类号： B01L3/502738 ,  B01L2200/142 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12M23/16 ,  C12M23/34

摘要： Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.

摘要翻译： 公开了用于细胞培养的微流体装置及其使用方法。 一个装置包括基底和膜。 衬底包括与通道流体连通的储存器。 可以将生物相容的流体添加到储存器和通道中。 储存器被配置为接收和保留细胞块的至少一部分。 膜作为生物相容性流体从通道蒸发的障碍。 可以加入覆盖流体以覆盖生物相容性流体以防止生物相容性流体的蒸发。

公开(公告)号：US20070090166A1

公开(公告)日：2007-04-26

申请号：US11582027

申请日：2006-10-17

申请人： Shuichi Takayama ,  Lourdes Cabrera ,  Yun Heo ,  Gary Smith

发明人： Shuichi Takayama ,  Lourdes Cabrera ,  Yun Heo ,  Gary Smith

IPC分类号： A47J36/02

CPC分类号： C12N5/0075 ,  B01L3/5027 ,  B01L3/5085 ,  C12M23/12 ,  C12M23/16 ,  C12M23/34

摘要： Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.

摘要翻译： 公开了用于细胞培养的微流体装置及其使用方法。 一个装置包括基底和膜。 衬底包括与通道流体连通的储存器。 可以将生物相容的流体添加到储存器和通道中。 储存器被配置为接收和保留细胞块的至少一部分。 膜作为生物相容性流体从通道蒸发的障碍。 可以加入覆盖流体以覆盖生物相容性流体以防止生物相容性流体的蒸发。

公开(公告)号：US07043382B2

公开(公告)日：2006-05-09

申请号：US10778125

申请日：2004-02-17

申请人： Yun Heo

发明人： Yun Heo

IPC分类号： G06F9/45

CPC分类号： G01R31/31715 ,  G01R31/3016 ,  G06F17/5031 ,  G11C29/50012 ,  G11C29/56004

摘要： A low voltage swing bus analysis method using a static timing analysis (STA) tool, which provides simple and accurate timing analysis verification. The low voltage swing bus analysis method is used in a static timing analysis (STA) tool. The STA tool receives a design file which executes the timing verification program then extracts the timing model for each cell, present in a design file during execution of the timing verification program, and calculates a timing for each node. The STA tool extracts a timing model for a sense amplifying flip flop, among cells present in the design file during execution of the timing verification program, from execution of a subroutine and calculating a timing for each node connected to the sense amplifying flip flop. The STA tool outputs the calculated timings.

摘要翻译： 一种使用静态时序分析（STA）工具的低压摆动总线分析方法，提供简单准确的时序分析验证。 在静态时序分析（STA）工具中使用低压摆幅总线分析方法。 STA工具接收执行定时验证程序的设计文件，然后在执行定时验证程序期间提取存在于设计文件中的每个单元的定时模型，并且计算每个节点的定时。 STA工具在执行定时验证程序期间提取设计文件中存在的单元中的感测放大触发器的定时模型，并且计算连接到感测放大触发器的每个节点的定时。 STA工具输出计算出的时间。