公开(公告)号：US06841361B1

公开(公告)日：2005-01-11

申请号：US09959548

申请日：2000-04-26

申请人： Shusaku Oka ,  Seiji Sato ,  Naohiko Higashikuni ,  Masaaki Kondo ,  Toshiyuki Kudo ,  Shigeaki Watanabe ,  Yoshihiro Waki ,  Hirotaka Yuki

发明人： Shusaku Oka ,  Seiji Sato ,  Naohiko Higashikuni ,  Masaaki Kondo ,  Toshiyuki Kudo ,  Shigeaki Watanabe ,  Yoshihiro Waki ,  Hirotaka Yuki

IPC分类号： C12N15/09 ,  C07K14/575 ,  C07K14/62 ,  C07K19/00 ,  C12N1/21 ,  C12N15/00 ,  C12N15/17 ,  C12P21/02 ,  C12R1/08 ,  C12P21/04

CPC分类号： C07K14/62 ,  C07K14/575 ,  C07K2319/00

摘要： This invention relates to a DNA encoding a fusion protein of formula (I) [Y]-[X1]-[B-chain]-[X2]-[Linker]-[X3]-[A-chain]  (I) wherein Y is a leader peptide sequence for the expression and secretion of the protein, comprising at least one amino acid residue; X1 is an amino acid sequence which is cleavable enzymatically or chemically; B-chain is the amino acid sequence of insulin B chain; X2 is an amino acid sequence which is cleavable enzymatically; Linker is a linker sequence comprising at least one amino acid residue; X3 is an amino acid sequence which is cleavable enzymatically; and A- chain is the amino acid sequence of insulin A chain, and to a method for producing insulin using an expression system containing the DNA in high efficiency and high yield.

摘要翻译： 本发明涉及编码式（I）的融合蛋白的DNA，其中Y是蛋白质的表达和分泌的前导肽序列，其包含至少一个氨基酸残基; X1是可酶切或化学切割的氨基酸序列 B链是胰岛素B链的氨基酸序列; X2是可酶切的氨基酸序列;接头是包含至少一个氨基酸残基的接头序列; X3是可酶切的氨基酸序列; A链是胰岛素A链的氨基酸序列，以及使用含有高效率和高收率的DNA的表达系统来生产胰岛素的方法。

公开(公告)号：US08570766B2

公开(公告)日：2013-10-29

申请号：US12956603

申请日：2010-11-30

申请人： Kazuya Noguchi ,  Hiromi Maeda ,  Toshimitsu Kobayashi ,  Atsushi Kaneko ,  Yoshihiro Waki ,  Masaki Yamamoto

发明人： Kazuya Noguchi ,  Hiromi Maeda ,  Toshimitsu Kobayashi ,  Atsushi Kaneko ,  Yoshihiro Waki ,  Masaki Yamamoto

IPC分类号： H05K9/00 ,  H05K5/06

CPC分类号： H05K5/061 ,  H05K9/0009

摘要： A shield case includes a first shield member and a second shield member coupled to the first shield member to form the shield case. A concave portion is capable of accommodating a sealing member for sealing inner space of the shield case by contacting the first shield member and the second shield member, and is formed on the second shield member. On each of the first shield member and the second shield member, a paint film portion is formed on an area of the shield case external to the concave portion. On the first shield member or the second shield member or combination thereof, a convex portion is formed on the area of the shield case on the inner space side and internal to the concave portion. The surfaces of the first shield member and the second shield member contact each other at the convex portion.

摘要翻译： 屏蔽壳包括耦合到第一屏蔽构件以形成屏蔽壳的第一屏蔽构件和第二屏蔽构件。 凹部能够容纳用于通过接触第一屏蔽部件和第二屏蔽部件来密封屏蔽壳体的内部空间的密封部件，并且形成在第二屏蔽部件上。 在第一屏蔽部件和第二屏蔽部件中的每一个上，在屏蔽壳体的凹部外侧的区域上形成有漆膜部。 在第一屏蔽构件或第二屏蔽构件或其组合上，在屏蔽壳体的内部空间侧的区域和凹部内部形成有凸部。 第一屏蔽件和第二屏蔽件的表面在凸部处彼此接触。

公开(公告)号：US20110128715A1

公开(公告)日：2011-06-02

申请号：US12956603

申请日：2010-11-30

申请人： Kazuya NOGUCHI ,  Hiromi Maeda ,  Toshimitsu Kobayashi ,  Atsushi Kaneko ,  Yoshihiro Waki ,  Masaki Yamamoto

发明人： Kazuya NOGUCHI ,  Hiromi Maeda ,  Toshimitsu Kobayashi ,  Atsushi Kaneko ,  Yoshihiro Waki ,  Masaki Yamamoto

IPC分类号： H05K9/00

CPC分类号： H05K5/061 ,  H05K9/0009

摘要： A shield case includes a first shield member and a second shield member coupled to the first shield member to form the shield case. a concave portion is capable of accommodating a sealing member for sealing inner space of the shield case by contacting the first shield member and the second shield member, and is formed on the second shield member. On each of the first shield member and the second shield member, a paint film portion is formed on an area of the shield case external to the concave portion. On the first shield member or the second shield member or combination thereof, a convex portion is formed on the area of the shield case on the inner space side and internal to the concave portion. The surfaces of the first shield member and the second shield member contact each other at the convex portion.

摘要翻译： 屏蔽壳包括耦合到第一屏蔽构件以形成屏蔽壳的第一屏蔽构件和第二屏蔽构件。 凹部能够容纳用于通过接触第一屏蔽构件和第二屏蔽构件来密封屏蔽壳体的内部空间的密封构件，并且形成在第二屏蔽构件上。 在第一屏蔽部件和第二屏蔽部件中的每一个上，在屏蔽壳体的凹部外侧的区域上形成有漆膜部。 在第一屏蔽构件或第二屏蔽构件或其组合上，在屏蔽壳体的内部空间侧的区域和凹部内部形成有凸部。 第一屏蔽件和第二屏蔽件的表面在凸部处彼此接触。

公开(公告)号：US6127519A

公开(公告)日：2000-10-03

申请号：US401432

申请日：1990-12-21

申请人： Ronald C. Orlowski ,  Satoshi Hanamura ,  Masahiko Marumoto ,  Kenji Sakamoto ,  Yoshihiro Waki

发明人： Ronald C. Orlowski ,  Satoshi Hanamura ,  Masahiko Marumoto ,  Kenji Sakamoto ,  Yoshihiro Waki

IPC分类号： A61K38/00 ,  A61K38/23 ,  A61P3/00 ,  A61P3/14 ,  A61P5/00 ,  A61P29/00 ,  A61P43/00 ,  C07K7/54 ,  C07K14/585 ,  C07K17/00

CPC分类号： C07K14/585 ,  A61K38/00

摘要： Peptides useful in the regulation of calcium metabolism are disclosed. Also disclosed are pharmaceutical compositions of matter containing such peptides as well as a method for the regulation of calcium metabolism in a patient in need of such treatment. The peptides contain modified or unmodified portions of an amino acid sequence at the 8- to 32-positions of native calcination.

摘要翻译： PCT No.PCT / US90 / 02143 Sec。 371 1990年12月21日第 102（e）1990年12月21日日期PCT提交1990年4月19日PCT公布。 公开号WO90 / 12809 日期1990年11月1日公开了可用于调节钙代谢的肽。 还公开了含有这些肽的物质的药物组合物以及在需要这种治疗的患者中调节钙代谢的方法。 肽在天然煅烧的8至32位含有氨基酸序列的修饰或未修饰部分。