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    DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs
    3.
    发明授权
    DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs 失效
    编码新融合蛋白的DNA和通过DNA表达制备有用多肽的方法

    公开(公告)号：US06506595B2

    公开(公告)日：2003-01-14

    申请号：US09280030

    申请日：1999-03-26

    申请人： Seiji Sato ,  Naohiko Higashikuni ,  Toshiyuki Kudo ,  Masaaki Kondo

    发明人： Seiji Sato ,  Naohiko Higashikuni ,  Toshiyuki Kudo ,  Masaaki Kondo

    IPC分类号： C12N1500

    CPC分类号： C12N15/625 ,  C07K14/605 ,  C07K14/61 ,  C07K14/62 ,  C07K14/655 ,  C07K2319/034 ,  C07K2319/21 ,  C07K2319/50 ,  C07K2319/75

    摘要： This invention relates to a DNA comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises: a sequence of signal peptide for Bacillus cell wall protein (CWP); a tag sequence for separation and purification of the fusion protein; a linker sequence; a sequence for chemical or enzymatic cleavage; and an exogenous polypeptide sequence, the sequences being linked in order, the signal peptide, tag and linker being optional sequences; and wherein the nucleotide sequence encoding a fusion protein is ligated to 3′-end of a nucleic acid sequence comprising a Bacillus promoter region; to a vector comprising the DNA; to a bacterium belonging to the genus Bacillus comprising the vector; and to a process for preparation of a useful polypeptide by culture of the bacterium.

    摘要翻译： 本发明涉及包含编码融合蛋白的核苷酸序列的DNA，其中融合蛋白包含：用于芽孢杆菌细胞壁蛋白（CWP）的信号肽序列; 用于分离和纯化融合蛋白的标签序列; 接头序列; 用于化学或酶切割的序列; 和外源多肽序列，序列依次连接，信号肽，标签和接头是任选的序列; 并且其中将编码融合蛋白的核苷酸序列连接到包含芽孢杆菌启动子区的核酸序列的3'-末端; 涉及包含该DNA的载体; 涉及属于包含载体的芽孢杆菌属的细菌; 以及通过细菌培养制备有用多肽的方法。

    Sexing method of bovine embryos
    4.
    发明授权
    Sexing method of bovine embryos 失效
    牛胚胎的性别方法

    公开(公告)号：US5661011A

    公开(公告)日：1997-08-26

    申请号：US458393

    申请日：1995-06-02

    申请人： Toshiyuki Kudo ,  Yoshiaki Itagaki ,  Seiji Sato ,  Shizuyo Sutou ,  Toyoo Nakamura

    发明人： Toshiyuki Kudo ,  Yoshiaki Itagaki ,  Seiji Sato ,  Shizuyo Sutou ,  Toyoo Nakamura

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6879

    摘要： The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.

    摘要翻译： 本发明提供可以容易地获得牛胚胎性别的PCR引物，并提供使用这些引物确定牛胚胎性别的实用，快速和可靠的方法。 用于确定牛胚胎性别的方法的特征在于区分通过用雄性特异性和性别中性引物的PCR扩增特异性DNA序列获得的PCR产物。 这些引物衍生自与牛雄性基因组特异性杂交的DNA，以及与牛雄性和雌性基因组性别中性杂交的DNA。

    Sexing method of bovine embyros
    6.
    发明授权
    Sexing method of bovine embyros 失效
    牛的embyros的性别的方法

    公开(公告)号：US5461145A

    公开(公告)日：1995-10-24

    申请号：US984044

    申请日：1992-12-02

    申请人： Toshiyuki Kudo ,  Yoshiaki Itagaki ,  Seiji Sato ,  Shizuyo Sutou ,  Toyoo Nakamura

    发明人： Toshiyuki Kudo ,  Yoshiaki Itagaki ,  Seiji Sato ,  Shizuyo Sutou ,  Toyoo Nakamura

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6879

    摘要： The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.

    摘要翻译： 本发明提供可以容易地获得牛胚胎性别的PCR引物，并提供使用这些引物确定牛胚胎性别的实用，快速和可靠的方法。 用于确定牛胚胎性别的方法的特征在于区分通过用雄性特异性和性别中性引物的PCR扩增特异性DNA序列获得的PCR产物。 这些引物衍生自与牛雄性基因组特异性杂交的DNA，以及与牛雄性和雌性基因组性别中性杂交的DNA。

    Tensioner assembly
    7.
    发明授权
    Tensioner assembly 有权
    张紧器总成

    公开(公告)号：US09388882B2

    公开(公告)日：2016-07-12

    申请号：US13947201

    申请日：2013-07-22

    申请人： Takehiro Takano ,  Seiji Sato

    发明人： Takehiro Takano ,  Seiji Sato

    IPC分类号： F16H7/12 ,  F16C33/78 ,  F16C35/063 ,  F16C13/00 ,  F16H7/20 ,  F16C33/58 ,  F16H7/08 ,  F16C19/06

    CPC分类号： F16H7/1281 ,  F16C13/006 ,  F16C19/06 ,  F16C33/586 ,  F16C33/7889 ,  F16C33/805 ,  F16C35/063 ,  F16C2226/60 ,  F16C2361/63 ,  F16H7/12 ,  F16H7/20 ,  F16H2007/0865 ,  F16H2007/0893

    摘要： A tensioner assembly includes a pulley arm pivotable about a pivot shaft, and a tension pulley mounted on the pulley arm at its end remote from the pivot shaft and pressed against a belt to keep the tension of the belt. The tension pulley is rotatably mounted around a base portion of the pulley arm through a rolling bearing with the inner surface and axial rear end surface of the bearing inner race in contact with the outer peripheral surface of the base portion and an end surface of a shoulder of the base portion, respectively. The base portion has an axial through hole formed with an internal thread on its inner surface. A support bolt is threaded into the through hole from the axial front end to press the rolling bearing against the end surface of the shoulder. The internal thread has an axial front end located in the vicinity of, or axially rearwardly of, the end surface of the shoulder.

    摘要翻译： 张紧器组件包括可围绕枢转轴线枢转的滑轮臂，以及安装在滑轮臂上的张力滑轮，其远端远离枢轴并被压靠皮带以保持皮带的张力。 张力滑轮通过滚动轴承可旋转地安装在滑轮臂的基部周围，轴承内圈的内表面和轴向后端表面与基部的外周表面和肩部的端面接触 的基部。 基部具有在其内表面上形成有内螺纹的轴向通孔。 支撑螺栓从轴向前端螺纹连接到通孔中，以将滚动轴承按压在肩部的端面上。 内螺纹具有位于肩部的端表面附近或轴向后方的轴向前端。

    MULTIBLADE CENTRIFUGAL FAN AND AIR CONDITIONER EQUIPPED WITH THE SAME
    8.
    发明申请
    MULTIBLADE CENTRIFUGAL FAN AND AIR CONDITIONER EQUIPPED WITH THE SAME 有权
    多功能离心式风扇和空调设备配备

    公开(公告)号：US20130092357A1

    公开(公告)日：2013-04-18

    申请号：US13806003

    申请日：2011-10-20

    申请人： Seiji Sato ,  Tsuyoshi Eguchi ,  Atusi Suzuki

    发明人： Seiji Sato ,  Tsuyoshi Eguchi ,  Atusi Suzuki

    IPC分类号： F04D29/42 ,  F28F13/12

    CPC分类号： F04D29/4226 ,  F04D29/403 ,  F04D29/4233 ,  F04D29/441 ,  F04D29/661 ,  F05D2250/52 ,  F05D2260/96 ,  F24F1/0022 ,  F24F13/24 ,  F28F13/12

    摘要： Provided are a low-noise, high-performance multiblade centrifugal fan that suppresses interference between rotational flows generated within extension sections that are extended in a rotation-axis direction at upper and lower end surfaces of a scroll casing, and an air conditioner equipped with the same. In a multiblade centrifugal fan having an impeller disposed in a rotatable manner about a rotation shaft within a scroll casing having a flow path whose cross section gradually increases in a rotational direction, at least one of upper and lower end surfaces of the scroll casing serves as an inclined end surface that is extended in the extending direction of the rotation shaft such that an extended height thereof gradually increases in the rotational direction from a scroll start position, and a stepped section extending in the rotational direction is provided between the inclined end surface and an inner-peripheral side surface of an extension section.

    摘要翻译： 提供一种低噪声，高性能的多叶片式离心风扇，其抑制在涡旋壳体的上端面和下端面上沿旋转轴线方向延伸的延伸部内产生的旋转流之间的干涉，以及装备有 相同。 在具有可旋转地设置在涡旋壳体内的旋转轴的叶片的多叶片离心式风扇中，所述涡旋壳体的横截面在旋转方向上逐渐增大，所述涡旋壳体的上端面和下端面中的至少一方作为 倾斜端面沿着旋转轴的延伸方向延伸，使得其延伸高度从涡旋开始位置沿旋转方向逐渐增大，并且在倾斜端面和 延伸部分的内周侧表面。

    MULTI-BLADE CENTRIFUGAL FAN AND AIR CONDITIONER EMPLOYING THE SAME
    9.
    发明申请
    MULTI-BLADE CENTRIFUGAL FAN AND AIR CONDITIONER EMPLOYING THE SAME 有权
    多叶离心风扇和空调使用者

    公开(公告)号：US20120211205A1

    公开(公告)日：2012-08-23

    申请号：US13318957

    申请日：2010-09-15

    申请人： Tsuyoshi Eguchi ,  Atsusi Suzuki ,  Seiji Sato ,  Masahiko Takahashi

    发明人： Tsuyoshi Eguchi ,  Atsusi Suzuki ,  Seiji Sato ,  Masahiko Takahashi

    IPC分类号： F04D29/44 ,  H01L23/467

    CPC分类号： F04D17/16 ,  F04D29/281 ,  F04D29/4226 ,  F04D29/441 ,  F04D29/681 ,  F05D2250/52

    摘要： In a multi-blade centrifugal fan in which an impeller is provided in a scroll casing in a freely rotatable manner, the scroll casing is provided with an axially expanded portion that forms an air channel at a bottom surface thereof which is expanded in a rotation-axis direction at a radially outer side of an annular flange portion which supports the impeller; and is provided, in a region of an outlet between a tongue portion and a spiral-end portion of the scroll casing in the axially expanded portion, with a protrusion that protrudes radially outward from a radially inner side surface by a predetermined amount so as to directly face an airflow in a circumferential direction.

    摘要翻译： 在涡旋壳体中以可自由旋转的方式设置叶轮的多叶片离心式风扇中，涡旋壳体设置有轴向膨胀部，该轴向扩张部在其底面形成有空气通道， 在支撑叶轮的环形凸缘部的径向外侧的轴线方向; 并且在轴向膨胀部分的涡旋壳体的舌部和螺旋端部之间的出口的区域中设置有从径向内侧表面径向向外突出预定量的突起，以便 直接面向圆周方向的气流。

    VEHICLE HEAT-EXCHANGE MODULE
    10.
    发明申请
    VEHICLE HEAT-EXCHANGE MODULE 有权
    车辆换热器模块

    公开(公告)号：US20120031591A1

    公开(公告)日：2012-02-09

    申请号：US13264644

    申请日：2010-09-10

    申请人： Tsuyoshi Eguchi ,  Atsusi Suzuki ,  Yoshinao Komatsu ,  Seiji Sato

    发明人： Tsuyoshi Eguchi ,  Atsusi Suzuki ,  Yoshinao Komatsu ,  Seiji Sato

    IPC分类号： F28F13/00

    CPC分类号： F04D29/384 ,  F04D29/681 ,  F05D2240/304

    摘要： In a vehicle heat-exchange module in which a fan unit is provided at the downstream side of a rectangular heat exchanger, and the fan unit is provided with a shroud having a bell-mouth and an annular opening, a propeller fan that is disposed in the annular opening, and a fan motor that rotationally drives the propeller fan, the fan unit is a unit having a single-fan configuration in which motor input power is at a predetermined level or less, and the propeller fan is provided with two sets of winglets that are respectively constructed upright, with a prescribed gap therebetween in the radial direction, along the circumferential direction on both a pressure surface and a suction surface of the root side of the blades.

    摘要翻译： 另外，在矩形热交换器的下游侧设置风扇单元的车辆用热交换模块中，风扇单元设置有具有喇叭口和环状开口的罩，所述风扇单元配置在 环形开口和旋转地驱动螺旋桨式风扇的风扇电动机，风扇单元是电动机输入功率在预定水平以下的单风扇结构的单元，并且螺旋桨式风扇设置有两组 在叶片的根侧的压力表面和吸力表面上沿着圆周方向分别构造成直立的小翼，在径向上具有规定的间隙。