公开(公告)号：US20040265880A1

公开(公告)日：2004-12-30

申请号：US10842649

申请日：2004-05-10

申请人： Sigma-Aldrich Co.

发明人： Laurel M. Donahue ,  Daniel W. Allison ,  Terrell K. Johnson ,  Kathryn A. Aboytes ,  Danny Fong

IPC分类号： C12Q001/68 ,  C12N005/00

CPC分类号： C12Q1/6837 ,  B01J2219/00378 ,  B01J2219/00385 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  C12N5/0018 ,  C12N5/0037

摘要： The present invention relates to a method for designing a cell culture medium adapted to support a cell line in a pre-defined manner. The method includes generating an expression profile of a cell line, identifying from the expression profile a set of biomolecules to evaluate for their effect on an end-point assay using the cell line, testing each biomolecule in the set for its effect in the endpoint assay, wherein each biomolecule that is determined to have a measurable effect in the end-point assay relative to a control, not containing the biomolecule, is considered a positive biomolecule and formulating a cell culture medium for the cell line by adding a positive biomolecule to a basal medium to form a modified medium, and determining whether the modified medium is sufficient to support the cell line in the pre-defined manner. Methods for identifying biomolecules for use in designing a cell culture medium adapted to support a cell line in a pre-defined manner, methods for preparing a serum-free cell culture medium that is sufficient to support a cell line and arrays for use in such methods are also provided.

摘要翻译： 本发明涉及一种用于以预定方式支持细胞系的细胞培养基的设计方法。 该方法包括产生细胞系的表达谱，从表达谱中鉴定一组生物分子，以评估其对使用细胞系的终点测定的影响，测试该组中的每个生物分子在终点测定中的作用 其中，相对于不包含生物分子的对照，在终点测定中确定具有可测量效应的每个生物分子被认为是阳性生物分子，并且通过向阳性生物分子添加阳性生物分子来配制细胞系的细胞培养基 基础培养基以形成修饰的培养基，并且确定修饰的培养基是否足以以预先确定的方式支持细胞系。 用于鉴定用于设计适于以预定方式支持细胞系的细胞培养基的生物分子的方法，用于制备足以支持细胞系的无血清细胞培养基的方法和用于该方法的阵列 也提供。