公开(公告)号：US20110203010A1

公开(公告)日：2011-08-18

申请号：US13061395

申请日：2008-09-02

申请人： Silvia Gómez Sebastián ,  Javier López Vidal ,  Ismael Sánchez Ramos ,  Covadonga Alonso Marti ,  José Angel Martinez Escribano

发明人： Silvia Gómez Sebastián ,  Javier López Vidal ,  Ismael Sánchez Ramos ,  Covadonga Alonso Marti ,  José Angel Martinez Escribano

IPC分类号： C12P21/00 ,  C12N15/63 ,  C12N5/10 ,  C12P19/34 ,  C07H21/00 ,  A01K67/033

CPC分类号： C12N15/85 ,  A01K67/033 ,  A01K2227/706 ,  C12N15/86 ,  C12N2710/14143 ,  C12N2800/105

摘要： Insect-derived promoters for foreign proteins expression in insect cells. Regulatory polynucleotide sequences which drive the expression of major insect proteins (hexamerins) at specific evolution stages of larva have been isolated from insects (Trichoplusia ni) in the present invention. Said regulatory polynucleotide sequences promote stronger foreign gene expression in the baculovirus system than the conventional polyhedrin promoter. Additionally, the combination of the new larva-derived promoters of the invention with the pL promoter increased baculovirus expression levels up to 61%-375% (depending on the time of infection) with respect to conventional baculoviruses used in the biotechnology industry. Promoter pB2 also drives gene expression at earlier times than polyhedrin promoter, being this feature a great advantage for correct protein folding and posttranslational modification.

摘要翻译： 昆虫细胞中外源蛋白表达的昆虫衍生启动子。 在本发明的昆虫（Trichoplusia ni）中已经分离了在幼虫的特定进化阶段驱动主要昆虫蛋​​白质（六聚体）表达的调节性多核苷酸序列。 所述调节多核苷酸序列在杆状病毒系统中促进比常规多角体蛋白启动子更强的外源基因表达。 此外，本发明的新的幼虫衍生的启动子与pL启动子的组合相对于在生物技术工业中使用的常规杆状病毒增加杆状病毒表达水平高达61％-375％（取决于感染时间）。 启动子pB2也比早期多基因启动子驱动基因表达，这对于正确的蛋白质折叠和翻译后修饰具有重要的优势。

公开(公告)号：US08557246B2

公开(公告)日：2013-10-15

申请号：US12922287

申请日：2008-03-14

申请人： José Angel Martínez Escribano ,  Andrés Wigdorovitz ,  Félix Gil Dones ,  Agustin Ostachuk ,  Mariano Pèrez Filguera ,  Javier Dominguez ,  Carmen Núnez Serrano ,  Maria Josè Dus Santos ,  Covadonga Alonso Marti

发明人： José Angel Martínez Escribano ,  Andrés Wigdorovitz ,  Félix Gil Dones ,  Agustin Ostachuk ,  Mariano Pèrez Filguera ,  Javier Dominguez ,  Carmen Núnez Serrano ,  Maria Josè Dus Santos ,  Covadonga Alonso Marti

IPC分类号： A01H5/00 ,  C12N5/02 ,  C12N15/00 ,  C12N1/20 ,  C07H21/02 ,  C07K14/00

CPC分类号： C07K14/005 ,  A61K39/0258 ,  A61K39/12 ,  A61K39/23 ,  A61K39/385 ,  A61K2039/55566 ,  A61K2039/605 ,  A61K2039/6056 ,  A61K2039/6075 ,  C07K16/2833 ,  C07K2317/34 ,  C07K2317/622 ,  C07K2319/33 ,  C07K2319/74 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2750/14322 ,  C12N2750/14334 ,  C12N2770/24322 ,  C12N2770/24334 ,  Y02A50/474

摘要： Fusion protein with directioning of vaccinal antigens toward antigen-presenting cells and the applications thereof. This invention relates to a gene construct that comprises, operatively bound, at least one nucleotide sequence (A) that encodes a polypeptide with SEQ ID NO: 1, which has a region that recognizes the β chain of the class-II DR antigen present on the surface of antigen-presenting cells; and one nucleotide sequence (B) that encodes a vaccinal antigen of interest. Moreover, this invention relates to recombinant vectors useful for the expression of the gene construct of the invention, transgenic cells and plants transformed or transfected with said vectors, fusion proteins encoded by the gene construct of the invention and vaccines that comprise said fusion proteins.

摘要翻译： 具有针对抗原呈递细胞的疫苗抗原方向的融合蛋白及其应用。 本发明涉及一种基因构建体，其包含可操作地结合至少一个编码具有SEQ ID NO：1的多肽的核苷酸序列（A），所述核苷酸序列具有识别存在于所述II类DR抗原上的β链的区域 抗原呈递细胞的表面; 和编码感兴趣的疫苗抗原的一个核苷酸序列（B）。 此外，本发明涉及可用于表达本发明的基因构建体的转基因载体，用所述载体转化或转染的转基因细胞和植物，由本发明的基因构建体编码的融合蛋白和包含所述融合蛋白的疫苗。