公开(公告)号：US20220341836A1

公开(公告)日：2022-10-27

申请号：US17639355

申请日：2020-09-03

Applicant: Singapore University of Technology and Design

Inventor： Ye AI ,  Dahou YANG

IPC: G01N15/10

Abstract: There is provided a microfluidic device for single cell processing including: a substrate; a fluidic channel provided in the substrate; and a plurality of electrodes arranged adjacent to the fluidic channel for determining a position of a cell in the fluidic channel, the plurality of electrodes comprising a pair of sensing electrodes comprising a first sensing electrode and a second sensing electrode, wherein at least the first sensing electrode of the pair of sensing electrodes extends in a first direction, the pair of sensing electrodes is configured to measure a differential electrical signal across a sensing region as the cell flows through the sensor portion of the fluidic channel; and a biasing electrode arranged between the first sensing electrode and the second sensing electrode, the biasing electrode being configured to receive a biasing voltage. One of the second sensing electrode and the biasing electrode extends in a direction at least substantially parallel to the first sensing electrode and the other one of the second sensing electrode and the biasing electrode is arranged to have a slanted orientation with respect to the first sensing electrode. There is also provided a method of forming the microfluidic device, and a method and a system for single cell processing using the microfluidic device.

公开(公告)号：US20210053061A1

公开(公告)日：2021-02-25

申请号：US16772991

申请日：2018-12-14

Applicant: SINGAPORE UNIVERSITY OF TECHNOLOGY AND DESIGN

Inventor： Ye AI ,  Yinning ZHOU

IPC: B01L3/00 ,  G01N15/02

Abstract: The present invention relates to the microfluidic sorting, separating and/or manipulation of particles, preferably circulating tumor cells (CTCs). In an aspect of the present invention, there is provided a device for sorting, separating or manipulating particles in a fluid suspension, the device comprising: (a) at least one inlet for introducing the fluid suspension; (b) at least one outlet for discharging the fluid suspension containing particles of a desired size; and (c) a channel in fluid communication with and intermediate the at least one inlet and the at least one outlet, a portion of the main channel is curved to form at least one curved unit, the curved unit is shaped to form a profile of a wave having a crest, a lip that curls over a trough, and a face, wherein the crest, lip, face and trough of the curved unit each forms a semicircular arc segment, the fluid suspension travels through the curved unit from the semicircular arc segment of the crest to the semicircular arc segment of the trough.

公开(公告)号：US20240307879A1

公开(公告)日：2024-09-19

申请号：US18562436

申请日：2022-05-27

Applicant: SINGAPORE UNIVERSITY OF TECHNOLOGY AND DESIGN

Inventor： Ye AI ,  Jianwei ZHONG

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/1031

CPC classification number: B01L3/502761 ,  G01N15/1031 ,  B01L2200/0652 ,  B01L2300/0645 ,  G01N2015/1006

Abstract: The invention relates to an impedance-based microfluidic flow cytometry device and a method of use thereof for determining a characteristic of a particle in a fluid suspension. Said device comprising: a channel comprising a sensing region to sense a particle flowing through the channel; an electrode arrangement disposed adjacent the sensing region, wherein the electrode arrangement is configured to generate at least one first region of differential current and at least one second region of differential current, and wherein the at least one first region and at least one second region have opposite phases of electric current. The electrode arrangement may be a double differential configuration having 5 electrodes in a coplanar arrangement, comprising a central electrode, two ground electrodes disposed adjacent the central electrode on opposite sides of the central electrode, and two end electrodes disposed adjacent the ground electrodes on the sides opposite to the central electrode.

公开(公告)号：US20230296491A1

公开(公告)日：2023-09-21

申请号：US18041724

申请日：2021-08-25

Applicant: SINGAPORE UNIVERSITY OF TECHNOLOGY AND DESIGN

Inventor： Ye AI ,  Minhui LIANG

IPC: G01N15/14

CPC classification number: G01N15/1427 ,  G01N15/1404 ,  G01N15/147 ,  G01N15/1475 ,  G01N15/1484 ,  G01N2015/1413 ,  G01N2015/1497

Abstract: The present invention relates to a device and method for high-throughput single cell stretching with the hydrodynamic force for assessing cellular mechanical properties. In an aspect of the invention, there is provided a uniquely designed microfluidic channel flowing with viscoelastic fluids, sensing electrodes integrated with the microchannel and a high-speed imaging and processing system. Cells are continuously pumped in the device, aligned and stretched. The arrival of individual cells prior to the cell stretching site can be detected by the electrical sensing unit, which produces a triggering signal to activate a high-speed camera for on-demand imaging of the cell motion and deformation. Cellular mechanical properties including cell size and cell deformability are extracted from the analysis of these captured single cell images.