公开(公告)号：US20240060094A1

公开(公告)日：2024-02-22

申请号：US18452169

申请日：2023-08-18

Applicant: SixRing Inc.

Inventor： Alexandra OSTASZEWSKI ,  Julie GREER ,  Alejandra ENRIQUEZ ,  Kyle G. WYNNYK ,  Andrew CORBETT ,  Markus WEISSENBERGER

IPC: C12P7/10

CPC classification number: C12P7/10

Abstract: A method of obtaining ethanol from a lignocellulosic biomass comprises delignification of a lignocellulosic biomass using a modified Caro's acid. Next a solid portion of the delignification reaction mixture is recovered wherein the solid portion comprises a substantially hemicellulose-free cellulosic component which includes at most, up to 15% w/w hemicellulose. Next, the recovered solid portion of the resulting reaction mixture is exposed to an enzyme mix comprising cellulase enzymes to create a saccharification system that breaks down the cellulose into a saccharified composition such as oligosaccharides. The saccharified composition is then fed to an organism, such as yeast, with the ability to ferment sugars into ethanol.

公开(公告)号：US20230279453A1

公开(公告)日：2023-09-07

申请号：US18117163

申请日：2023-03-03

Applicant: SixRing Inc.

Inventor： Alejandra ENRIQUEZ ,  Julie GREER ,  Alexandra OSTASZEWSKI ,  Markus WEISSENBERGER

IPC: C12P19/12 ,  C12P19/02 ,  C12P7/14 ,  C12N1/20 ,  C12P19/14

CPC classification number: C12P19/12 ,  C12P19/02 ,  C12P7/14 ,  C12N1/20 ,  C12P19/14

Abstract: A process to hydrolyze cellulose into cellobiose comprising the following steps:



providing a reaction vessel;
providing a Cellulomonas uda (ATCC 491) inoculum;
exposing said Cellulomonas uda (ATCC 491) bacterium to a source of cellulose having a kappa number of less than 10 in an aqueous medium of pH of about 8 at a temperature ranging from 30° C. to 35° C. for a period of time ranging from 14 to 42 days;
exposing the cellobiose to a bacterium or fungi or yeast, or combination which converts cellobiose to glucose or ethanol.

公开(公告)号：US20240294961A1

公开(公告)日：2024-09-05

申请号：US18460258

申请日：2023-09-01

Applicant: SixRing Inc.

Inventor： Alejandra ENRIQUEZ ,  Julie GREER ,  Markus WEISSENBERGER ,  Alexandria OSTASZEWSKI

IPC: C12P19/12 ,  C12N9/42

CPC classification number: C12P19/12 ,  C12N9/244 ,  C12N9/2445 ,  C12Y302/01006 ,  C12Y302/01021

Abstract: A process to hydrolyze cellulose into cellobiose comprises providing a reaction vessel and providing an inoculum of a bacteria or fungus capable of expressing one or more endo or exo-β-glucanase into reaction vessel. The bacterium or fungus is exposed to a source of cellulose having a kappa number of less than 10 and a hemicellulose content of less than 15% in an aqueous medium of pH between 5 and 9 at a temperature ranging from 20° C. to 40° C. for a period of time ranging from 1 to 30 days. The cellobiose is exposed to a bacterium or fungi or yeast, or combination which converts cellobiose to glucose or ethanol.

公开(公告)号：US20240294960A1

公开(公告)日：2024-09-05

申请号：US18460219

申请日：2023-09-01

Applicant: SixRing Inc.

Inventor： Alejandra ENRIQUEZ ,  Julie GREER ,  Markus WEISSENBERGER ,  Alexandra OSTASZEWSKI

IPC: C12P19/02

CPC classification number: C12P19/02 ,  C12Y302/01004 ,  C12Y302/01021

Abstract: A process to obtain glucose from a pretreated lignocellulosic biomass comprises providing a lignocellulosic biomass and contacting said lignocellulosic biomass to a modified Caro's acid composition for a period of time necessary to remove more than 98.5% of the lignin present in the lignocellulosic biomass and thus obtaining a solid stream and a liquid stream. The solid stream is exposed to an enzyme blend to produce a hydrolysate comprising sugars obtained from the hydrolysis of cellulose and hemicellulose. Optionally, the hydrolysate may be fermented to produce value-added products.

公开(公告)号：US20240052375A1

公开(公告)日：2024-02-15

申请号：US18447863

申请日：2023-08-10

Applicant: SixRing Inc.

Inventor： Corynne O'FARRELL ,  Julie GREER ,  Alexandra OSTASZEWSKI ,  Markus WEISSENBERGER ,  Alejandra ENRIQUEZ

IPC: C12P5/02 ,  C12M1/107

CPC classification number: C12P5/023 ,  C12M21/04

Abstract: A process to increase the amount of methane produced from a bio-digester comprises providing a digester to receive a feed and to capture a biogas composition resulting from bio-digestion. Organic material is added to the digester along with at least one inoculum capable of converting a portion of the organic material into methane under anaerobic conditions. A biomass is provided that is substantially free of lignin in an amount that ranges between 0.1 to 5% w/w of the total feed, the biomass further having another organic material. The biomass is added to the digester. Sufficient time is allowed for the digester to degrade the biomass and the organic material to yield a biogas composition that includes methane. Optionally, the biomass that is substantially free of lignin is added in an amount that ranges between 0.1 to 5% w/w of the total feed of the biomass having the other organic material.