Colour management for biological samples
    1.
    发明授权
    Colour management for biological samples 有权
    生物样品的色彩管理

    公开(公告)号:US08649581B2

    公开(公告)日:2014-02-11

    申请号:US13124693

    申请日:2009-10-16

    IPC分类号: G06K9/00

    CPC分类号: G06T11/001 H04N1/62

    摘要: A method of determining a color transformation for images of biological material includes preparing a first set of biological test objects using a first preparation method, and preparing a second set of biological test objects using a second preparation method. Each test object in the second set of test objects corresponding to a counterpart test object in the first set of test objects, the test object and its counterpart being of the same biological type of material. For each test object in the first and second set of test objects, the color of the test object is determined thereby generating a first and second set of colors The method further includes generating a conversion table indicating a mapping between the colors in the first set of colors and the corresponding colors in the second set of colors. The first and second preparation methods include first and second staining methods, respectively.

    摘要翻译: 确定生物材料的图像的颜色变换的方法包括使用第一制备方法制备第一组生物测试对象,并使用第二制备方法制备第二组生物测试对象。 第二组测试对象中的每个测试对象对应于第一组测试对象中的对应测试对象,测试对象及其对应物具有相同的生物类型的材料。 对于第一和第二组测试对象中的每个测试对象,确定测试对象的颜色,从而生成第一和第二组颜色。该方法还包括生成表示第一组中的颜色之间的颜色之间的映射的转换表 颜色和相应的颜色在第二组颜色。 第一和第二种制备方法分别包括第一和第二染色方法。

    Beam shaping without introducing divergence within a light beam
    2.
    发明授权
    Beam shaping without introducing divergence within a light beam 有权
    光束成形,而不会在光束内引入发散

    公开(公告)号:US08421000B2

    公开(公告)日:2013-04-16

    申请号:US12743024

    申请日:2008-11-20

    IPC分类号: G02F1/01 G02B5/30

    CPC分类号: G01N21/6458 G02B5/1833

    摘要: An optical beam shaper comprises a polarization-dependent phase adjustment member which applies a phase pattern of equal magnitude and opposite sign to two orthogonal polarization states. In a preferred embodiment the beamer shaper is a dif tractive element made of a birefringent material, such as a photo-polymerizable liquid crystal polymer frozen in a uniaxial alignment, said dif tractive element comprising a plurality of zones, each zone having a stepped thickness defining a plurality of steps. The beam shaper can be used to introduce astigmatism to a polarized light beam or to undo the astigmatism to a beam with an orthogonal polarization state. The beam shaper is advantageously used within a detection device, such as a fluorescence scanner.

    摘要翻译: 光束整形器包括偏振相关相位调整构件,其对两个正交偏振状态施加相等幅度和相反符号的相位图案。 在优选实施例中,光束整形器是由双折射材料制成的吸引元件,例如以单轴取向冷冻的光可聚合液晶聚合物,所述吸引元件包括多个区域,每个区域具有限定 多个步骤。 光束整形器可用于对偏振光束引入像散或者使具有正交偏振状态的光束的散光消除。 光束整形器有利地用在诸如荧光扫描仪的检测装置内。

    TWO-DIMENSIONAL ARRAY OF RADIATION SPOTS FOR AN OPTICAL SCANNING DEVICE
    4.
    发明申请
    TWO-DIMENSIONAL ARRAY OF RADIATION SPOTS FOR AN OPTICAL SCANNING DEVICE 审中-公开
    用于光学扫描装置的两维辐射阵列阵列

    公开(公告)号:US20110019064A1

    公开(公告)日:2011-01-27

    申请号:US12933166

    申请日:2009-03-16

    申请人: Sjoerd Stallinga

    发明人: Sjoerd Stallinga

    IPC分类号: H04N5/225 G02B21/06

    CPC分类号: G02B21/004 G02B26/10

    摘要: The invention relates to an optical scanning device (10) comprising: a spot generator (20) for generating a two-dimensional array (8) of radiation spots at lattice points Pmn=mT1+nT2 (m=1 to L1, n=1 to L2) where T 1 is a first lattice vector and T2 is a second lattice vector, and scanning means for scanning a sample (26) through the array of radiation spots in a scanning direction such that the radiation spots trace essentially equidistant lines (81, 82, 83) relative to the sample. According to the invention, the angle γ between the scanning direction and the first lattice vector T1 is at most as large as the angle between the scanning direction and the second lattice vector T2, and the ratio L1/L2 is less than 0.6. According to a preferred embodiment, L1 differs from Λ by less then 1.0 or L1 equals Λ with a tolerance of 10% or, Λ being defined by √2 D/R=(1+Λ2) Λ, D being the length of a lattice diagonal and R being the resolution. The invention further relates to an optical scanning method.

    摘要翻译: 本发明涉及一种光学扫描装置(10),包括:点阵发生器(20),用于在格点Pmn = mT1 + nT2(m = 1至L1,n = 1)处产生辐射点的二维阵列(8) 到L2),其中T 1是第一晶格矢量,T2是第二晶格矢量;以及扫描装置,用于通过扫描方向上的辐射点阵列扫描样品(26),使得辐射点基本上等距线(81 ,82,83)。 根据本发明,扫描方向与第一格子矢量T1之间的角度γ与扫描方向和第二格子矢量T2之间的角度最大,并且比率L1 / L2小于0.6。 根据优选实施例,L1与Λ不同于1.0或L1等于Λ,其公差为10%,或Λ由√2D / R =(1 +Λ2)Λ定义,D是格子的长度 对角线和R是分辨率。 本发明还涉及一种光学扫描方法。

    MULTI-MODAL SPOT GENERATOR AND MULTI-MODAL MULTI-SPOT SCANNING MICROSCOPE
    5.
    发明申请
    MULTI-MODAL SPOT GENERATOR AND MULTI-MODAL MULTI-SPOT SCANNING MICROSCOPE 审中-公开
    多模式发射器和多模式多点扫描显微镜

    公开(公告)号:US20100277580A1

    公开(公告)日:2010-11-04

    申请号:US12742978

    申请日:2008-11-19

    摘要: The invention relates to a spot generator (10) having: —an entrysurface (12) for receiving an incident light beam (20) and an exit surface (14) for (22, 24) transmitting the light beam, the entry surface defining an entryside (16) and the exit surface defining an exit side (18). According to the invention, the spot generator is designed to modulate the incident light beam to generate on the exit side a first plurality (22) and a second plurality (24) of separate light spots, each light spot belonging to the first plurality having a first angular spectrum and each light spot belonging to the second plurality having a second angular spectrum different than the first angular spectrum. Advantageously, the spot generator comprises a periodic binary phase structure. The invention further relates to a multi-spot scanning microscope and to a method of imaging a microscopic sample.

    摘要翻译: 本发明涉及一种点发生器(10),具有: - 入射表面(12),用于接收入射光束(20)和用于(22,24)透射光束的出射表面(14),入射表面限定一个 入口侧(16)和出口表面限定出口侧(18)。 根据本发明,点发生器被设计成调制入射光束,以在出射侧产生第一多个(22)和第二多个(24)个分开的光点,每个属于第一多个光点的光点具有一个 第一角谱和属于第二多个的每个光点具有不同于第一角谱的第二角谱。 有利地,点发生器包括周期性二进制相位结构。 本发明还涉及一种多点扫描显微镜和一种对微观样品进行成像的方法。

    Phase-Conjugate Read-Out in a Holographic Data Storage
    7.
    发明申请
    Phase-Conjugate Read-Out in a Holographic Data Storage 审中-公开
    全息数据存储中的相位共轭读取

    公开(公告)号:US20080253257A1

    公开(公告)日:2008-10-16

    申请号:US11570535

    申请日:2005-06-20

    申请人: Sjoerd Stallinga

    发明人: Sjoerd Stallinga

    IPC分类号: G11B7/00

    CPC分类号: G11B7/0065 G11C13/042

    摘要: The invention relates to a holographic data storage medium (4) in which there are sections (20) which are used for data storage and sections (21) which are not. A diffractive structure (30a, 30b) is provided in respect of one or more of the unused sections (21), possibly at each of the boundaries between the used (20) and unused sections (21) or within the volume of a respective unused section (21) of the data storage volume. During read-out, a reference wave (6) is diffracted by the diffractive structure (30b) such that it enters a used section (20) (from the right) in a direction substantially perpendicular to the optical axis (16) of the signal wave (26). During recording, a reference wave (2) is diffracted by the diffractive structure (30a) such that it enters the used section (20) (from the left) in a direction substantially perpendicular to the optical axes (1b) of the signal wave (1).

    摘要翻译: 本发明涉及一种全息数据存储介质(4),其中存在用于数据存储的部分(20)和不是的部分(21)。 关于一个或多个未使用部分(21),可能在使用的(20)和未使用部分(21)之间的每个边界处或在一个或多个未使用部分(21)的体积内,提供衍射结构(30a,30b) 各个未使用部分(21)的数据存储卷。 在读出期间,参考波(6)被衍射结构(30b)衍射,使得其在基本上垂直于所述光轴(16)的方向(从右侧)进入使用部分(20) 信号波(26)。 在记录期间,参考波(2)被衍射结构(30a)衍射,使得其在基本上垂直于信号的光轴(1b)的方向上进入使用部分(20)(从左侧) 波(1)。

    Optical Scanning Device
    10.
    发明申请
    Optical Scanning Device 审中-公开
    光学扫描装置

    公开(公告)号:US20080117786A1

    公开(公告)日:2008-05-22

    申请号:US11813099

    申请日:2006-01-06

    IPC分类号: G11B7/00

    CPC分类号: G11B7/1353 G11B7/1275

    摘要: An optical scanning device for scanning a first optical record carrier, a second, different, optical record carrier and a third, different, optical record carrier, each record carrier having an information layer. The scanning device includes a radiation source system arranged to produce first, second and third radiation beams for scanning the first, second and third record carriers with first, second and third wavelengths, respectively; and a diffraction structure (34) arranged to introduce a first, second and third wavefront modification into the first, second and third radiation beams, respectively. The diffraction structure includes a surface having a plurality of steps (38), each step having a step height (hj) which controls a form of the first, second and third wavefront modifications, characterized in that the diffraction structure is formed from a material having an Abbe number of less than 50, whereby dispersion is provided between the first, second and third wavelengths in order to further control the form of the first, second and third wavefront modifications.

    摘要翻译: 一种用于扫描第一光学记录载体,第二个不同的光学记录载体和第三个不同的光学记录载体的光学扫描装置,每个记录载体具有信息层。 扫描装置包括一个辐射源系统,被布置成产生用于分别用第一,第二和第三波长扫描第一,第二和第三记录载体的第一,第二和第三辐射束; 以及衍射结构(34),其被布置成分别将第一,第二和第三波前修改引入到第一,第二和第三辐射束中。 衍射结构包括具有多个步骤(38)的表面,每个步骤具有控制第一,第二和第三波前修改形式的台阶高度(h SUB),其特征在于, 衍射结构由阿贝数小于50的材料形成,从而在第一,第二和第三波长之间提供色散,以进一步控制第一,第二和第三波前修改的形式。