Device and method for amplifying and detecting target nucleic acids
    1.
    发明授权
    Device and method for amplifying and detecting target nucleic acids 失效
    用于扩增和检测靶核酸的装置和方法

    公开(公告)号:US5645801A

    公开(公告)日:1997-07-08

    申请号:US516728

    申请日:1995-08-18

    CPC分类号: B01L7/52 Y10S435/81

    摘要: Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then sealably mated with a detection chamber to form a sealed reaction/detection unit that is virtually irreversibly closed. One or more heating elements of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Kits include the reaction/detection units and reagents for amplification.

    摘要翻译: 提供了用于扩增和检测核酸的方法,装置,装置和试剂盒。 该装置是与反应/检测单元结合操作的热循环装置。 将样品装载到装置的反应室中,然后与检测室密封地配合,以形成几乎不可逆地封闭的密封的反应/检测单元。 热循环装置的一个或多个加热元件对反应/检测装置施加所需的温度以放大样品中的靶核酸。 然后将反应混合物转移到检测室,并将扩增的靶核酸固定在检测室中的载体上。 与该装置相关的检测系统检测并分析固定的扩增的核酸靶。 试剂盒包括用于扩增的反应/检测单元和试剂。

    Chassis connector and circuit board clip
    2.
    发明授权
    Chassis connector and circuit board clip 失效
    机箱连接器和电路板夹

    公开(公告)号:US3996500A

    公开(公告)日:1976-12-07

    申请号:US586870

    申请日:1975-06-16

    申请人: Ronald A. Coules

    发明人: Ronald A. Coules

    IPC分类号: H05K7/12 F16B2/20 F16B5/06

    CPC分类号: H05K7/12 Y10T24/3439

    摘要: A chassis connector and circuit board clip for electronic equipment, said clip having a pair of upright spaced apart legs, each having anchor means for mounting in a slotted chassis, a wall bridging the legs for supporting a circuit board spaced apart from and in alignment with a connector, and a locking tooth carried by one of the legs for holding the edge of an aligned circuit board relative to the chassis and connector. The clip is molded in one piece from plastic dielectric insulating material and one modification thereof also includes means for securing the connector to the chassis.

    摘要翻译: 一种用于电子设备的底盘连接器和电路板夹,所述夹具具有一对直立间隔开的支腿,每个支腿具有用于安装在开槽底盘中的锚定装置,桥接腿部的壁,用于支撑与第二支撑板间隔开并与之对准的电路板 一个连接器,以及一个腿部承载的锁定齿,用于保持对准的电路板相对于底盘和连接器的边缘。 夹子由塑料电介质绝缘材料模制成一体,其一个变型还包括用于将连接器固定到底架上的装置。

    Wheel having built-in hub
    4.
    发明授权
    Wheel having built-in hub 失效
    车轮内置中枢

    公开(公告)号:US4358162A

    公开(公告)日:1982-11-09

    申请号:US287994

    申请日:1981-07-29

    IPC分类号: B60B5/02 B60B37/10 B60B1/06

    CPC分类号: B60B37/10 B60B5/02

    摘要: A wheel is disclosed that has a built-in hub for receiving an axle. The wheel includes an annular outer rim, and a pair of circular webs that are received in the rim.Each of the webs includes an inner annular ring. The annular ring on one of the webs defines a through-passageway, and the annular ring on the other web defines an aperture having an open end that communicates with the passageway and an opposite closed end. The closed end of the other web is defined by a wall of the other web. The wall of the other web, together with the annular ring on the other web, define a built-in hub for limiting insertion of the axle onto the wheel and for covering the axle. The need for a separate hub component that can fall off and become lost is thereby eliminated.

    摘要翻译: 公开了一种具有用于接收轴的内置轮毂的车轮。 轮包括环形外轮缘和一对接收在轮辋内的圆形腹板。 每个腹板包括内环形环。 一个腹板上的环形环限定了通道,而另一个腹板上的环形环限定了具有与通道相通的开口端和相对的封闭端的孔。 另一个网的封闭端由另一个网的墙限定。 另一个腹板的壁与另一个腹板上的环形圈一起限定了一个内置轮毂,用于限制车轴插入车轮并覆盖车轴。 因此,消除了可能脱落并变得损失的单独的毂部件的需要。

    Quarter turn circuit board guide
    5.
    发明授权
    Quarter turn circuit board guide 失效
    四分之一回路电路板指南

    公开(公告)号:US4192478A

    公开(公告)日:1980-03-11

    申请号:US891021

    申请日:1978-03-28

    申请人: Ronald A. Coules

    发明人: Ronald A. Coules

    IPC分类号: F16M13/00

    摘要: A support for securing a circuit board on an apertured panel, wherein the support has a slot for slideably receiving and a tab for releaseably holding a circuit board therein, and a quarter turn fastener engageable in the panel aperture, said fastener and aperture having cooperating means for receiving said support and locking means for mounting the fastener upright on the panel upon manipulating the fastener a quarter turn in the aperture.

    摘要翻译: 一种用于将电路板固定在有孔面板上的支撑件,其中所述支撑件具有用于可滑动接收的槽和用于可释放地将电路板保持在其中的突片和可接合在所述面板孔中的四分之一转弯紧固件,所述紧固件和孔具有配合装置 用于接收所述支撑和锁定装置,用于在将所述紧固件操纵所述孔中的四分之一圈时将所述紧固件直立地安装在所述面板上。

    Quarter turn locking fastener device
    7.
    发明授权
    Quarter turn locking fastener device 失效
    四分之一圈锁紧紧固装置

    公开(公告)号:US4007516A

    公开(公告)日:1977-02-15

    申请号:US578474

    申请日:1975-05-19

    申请人: Ronald A. Coules

    发明人: Ronald A. Coules

    IPC分类号: F16B21/04 A44B17/00 F16B13/04

    CPC分类号: F16B21/04

    摘要: A quarter turn locking fastener device consisting of a socket part adapted to snap engage into an apertured chassis and to support an apertured panel spaced apart from the chassis, and a complemental stud part adapted for selected locking and unlocking engagement through the panel aperture and into the socket part upon a quarter turn of the stud part relative to the socket part to removably secure the panel spaced apart from and on the chassis and to lock the socket part to the chassis when the stud part is in locked condition.

    摘要翻译: 一种四分之一转动锁紧紧固装置,其包括适于卡扣接合到有孔底盘并且支撑与底盘间隔开的有孔面板的插座部分,以及互补螺柱部件,其适合于通过面板孔选择的锁定和解锁接合,并进入 插座部分相对于插座部分四分之一圈的螺柱部分,以可拆卸地固定与底架间隔开的面板,并且当螺柱部分处于锁定状态时将插座部分锁定到底架。

    Locking stand off
    8.
    发明授权
    Locking stand off 失效
    锁定支架

    公开(公告)号:US4297769A

    公开(公告)日:1981-11-03

    申请号:US107498

    申请日:1979-12-26

    申请人: Ronald A. Coules

    发明人: Ronald A. Coules

    IPC分类号: F16B5/06 F16B21/08 F16B19/00

    摘要: A one-piece fastener has an upper end that is detachably secured to a first panel and a lower end that is secured to a second panel.The lower end includes a flange that is receivable in an aperture in the second panel and bears against the wall of the aperture to minimize rocking movement of the fastener relative to the second panel. A lower portion of the flange is tapered to facilitate alignment of the flange within the aperture in the second panel.

    摘要翻译: 一体式紧固件具有可拆卸地固定到第一面板的上端和固定到第二面板的下端。 下端包括可接收在第二面板中的孔中的凸缘,并且抵靠孔的壁以最小化紧固件相对于第二面板的摆动运动。 凸缘的下部是锥形的,以便于凸缘在第二面板的孔内对准。

    Circuit board ejector
    9.
    发明授权
    Circuit board ejector 失效
    电路板喷射器

    公开(公告)号:US3952232A

    公开(公告)日:1976-04-20

    申请号:US475421

    申请日:1974-06-03

    申请人: Ronald A. Coules

    发明人: Ronald A. Coules

    IPC分类号: H05K7/14 H02B1/02

    CPC分类号: H05K7/1409 Y10T16/4713

    摘要: A circuit board ejector for snap-on attachment to an apertured circuit board or other electronic component slidably mounted in a rack or chassis, operable to initiate sliding of the circuit board for removal from or insertion into the chassis, and to a chassis -- circuit board combination including such ejectors.

    摘要翻译: 一种用于卡扣连接到有孔电路板或可滑动地安装在机架或机架中的其他电子部件的电路板顶出器,其可操作以启动电路板的滑动以便从底架中移除或插入到底盘中,以及机架 - 电路板 组合包括这种喷射器。