公开(公告)号：US09164086B2

公开(公告)日：2015-10-20

申请号：US13702252

申请日：2011-10-18

申请人： Stefan W. Hell ,  Shamil Nizamov ,  Gerald Donnert ,  Kirill Kolmakov ,  Heiko Schill ,  Lars Kastrup ,  Christian A. Wurm ,  Vladimir N. Belov ,  Johanna Wildanger ,  Katrin Willig

发明人： Stefan W. Hell ,  Shamil Nizamov ,  Gerald Donnert ,  Kirill Kolmakov ,  Heiko Schill ,  Lars Kastrup ,  Christian A. Wurm ,  Vladimir N. Belov ,  Johanna Wildanger ,  Katrin Willig

IPC分类号： C09B57/02 ,  G01N33/52 ,  C09B11/24 ,  C09B57/10 ,  G01N33/58 ,  G01N33/533

CPC分类号： G01N33/52 ,  C09B11/24 ,  C09B57/02 ,  C09B57/10 ,  G01N33/533 ,  G01N33/582 ,  G01N2458/00

摘要： The invention relates to novel fluorescent dyes with phosphorylated hydroxymethyl groups, a method for preparing the same as well as to their use in imaging techniques. The fluorescent dyes are coumarin, rhodamine or BODIPY dyes having of one of the following general formulae I-III: wherein W=OP(O)Y1Y2 or P(O)Y1Y2, where Y1 and Y2 independently denote any of the following residues: OH, O(−), ORa and ORb, NHRa and NHRb, NRaRb and NRcRd, ORa and NHRb, ORa and NRbRc, NHRa and NRbRc; and any salt thereof.

摘要翻译： 本发明涉及具有磷酸化羟甲基的新型荧光染料，其制备方法以及其在成像技术中的应用。 荧光染料是具有以下通式I-III之一的香豆素，罗丹明或BODIPY染料：其中W = OP（O）Y1Y2或P（O）Y1Y2，其中Y1和Y2独立地表示以下任何残基：OH ，O（ - ），ORa和ORb，NHRa和NHRb，NRaRb和NRcRd，ORa和NHRb，ORa和NRbRc，NHRa和NRbRc; 及其任何盐。

公开(公告)号：US20140220588A1

公开(公告)日：2014-08-07

申请号：US13702252

申请日：2011-10-18

申请人： Stefan W. Hell ,  Shamil Nizamov ,  Gerald Donnert ,  Kirill Kolmakov ,  Heiko Schill ,  Lars Kastrup ,  Christian A. Wurm ,  Vladimir N. Belov ,  Johanna Bueckers ,  Katrin Willig

发明人： Stefan W. Hell ,  Shamil Nizamov ,  Gerald Donnert ,  Kirill Kolmakov ,  Heiko Schill ,  Lars Kastrup ,  Christian A. Wurm ,  Vladimir N. Belov ,  Johanna Bueckers ,  Katrin Willig

IPC分类号： G01N33/52

CPC分类号： G01N33/52 ,  C09B11/24 ,  C09B57/02 ,  C09B57/10 ,  G01N33/533 ,  G01N33/582 ,  G01N2458/00

摘要： The invention relates to novel fluorescent dyes with phosphorylated hydroxymethyl groups, a method for preparing the same as well as to their use in imaging techniques. The fluorescent dyes are coumarin, rhodamine or BODIPY dyes having of one of the following general formulae I-III: wherein W=OP(O) Y1Y2 or P(O) Y1Y2, where Y1 and Y2 independently denote any of the following residues: OH, O(−), ORa and ORb, NHRa and NHRb, NRaRb and NRcRd, ORa and NHRb, ORa and NRbRc, NHRa and NRbRc; and any salt thereof.

摘要翻译： 本发明涉及具有磷酸化羟甲基的新型荧光染料，其制备方法以及其在成像技术中的应用。 荧光染料是具有以下通式I-III之一的香豆素，罗丹明或BODIPY染料：其中W = OP（O）Y1Y2或P（O）Y1Y2，其中Y1和Y2独立地表示以下任何残基：OH ，O（ - ），ORa和ORb，NHRa和NHRb，NRaRb和NRcRd，ORa和NHRb，ORa和NRbRc，NHRa和NRbRc; 及其任何盐。

公开(公告)号：US07863585B2

公开(公告)日：2011-01-04

申请号：US12706388

申请日：2010-02-16

申请人： Stefan W. Hell ,  Katrin Willig

发明人： Stefan W. Hell ,  Katrin Willig

IPC分类号： G01N21/64

CPC分类号： G01N21/6458 ,  G01N21/636 ,  G02B21/0076

摘要： A method of high spatial resolution imaging a structure in a sample comprises: marking the structure with molecules of a fluorescent dye; selecting a first wavelength for excitation light which excites the molecules of the fluorescent dye via a multi photon process for spontaneous emission of fluorescent light; focusing pulses of the excitation light into the sample to excite those molecules of the fluorescent dye present in a focal area of the focused excitation light; selecting a second wavelength shorter than the first wavelength for de-excitation light which de-excites excited molecules of the fluorescent dye prior to their spontaneous emission; during a plurality of the pulses of the excitation light, continuously directing the de-excitation light onto the sample to de-excite excited molecules of the fluorescent dye, which are located outside an measurement area which is a fraction of the focal area; and recording the fluorescent light spontaneously emitted by the molecules of the fluorescent dye in the sample.

摘要翻译： 对样品中的结构进行高空间分辨率成像的方法包括：用荧光染料的分子标记结构; 选择激发光的第一波长，其通过用于自发发射荧光的多光子过程激发荧光染料的分子; 将激发光的脉冲聚焦到样品中以激发存在于聚焦激发光的焦点区域中的荧光染料的那些分子; 选择比第一波长短的第二波长用于去激发荧光染料在其自发发射之前激发分子的去激发光; 在激发光的多个脉冲期间，将去激发光连续地引导到样品上以去激发位于焦点区域的一部分的测量区域外的荧光染料的激发分子; 并记录由荧光染料分子自发发射的荧光。