公开(公告)号：US09164086B2

公开(公告)日：2015-10-20

申请号：US13702252

申请日：2011-10-18

申请人： Stefan W. Hell ,  Shamil Nizamov ,  Gerald Donnert ,  Kirill Kolmakov ,  Heiko Schill ,  Lars Kastrup ,  Christian A. Wurm ,  Vladimir N. Belov ,  Johanna Wildanger ,  Katrin Willig

发明人： Stefan W. Hell ,  Shamil Nizamov ,  Gerald Donnert ,  Kirill Kolmakov ,  Heiko Schill ,  Lars Kastrup ,  Christian A. Wurm ,  Vladimir N. Belov ,  Johanna Wildanger ,  Katrin Willig

IPC分类号： C09B57/02 ,  G01N33/52 ,  C09B11/24 ,  C09B57/10 ,  G01N33/58 ,  G01N33/533

CPC分类号： G01N33/52 ,  C09B11/24 ,  C09B57/02 ,  C09B57/10 ,  G01N33/533 ,  G01N33/582 ,  G01N2458/00

摘要： The invention relates to novel fluorescent dyes with phosphorylated hydroxymethyl groups, a method for preparing the same as well as to their use in imaging techniques. The fluorescent dyes are coumarin, rhodamine or BODIPY dyes having of one of the following general formulae I-III: wherein W=OP(O)Y1Y2 or P(O)Y1Y2, where Y1 and Y2 independently denote any of the following residues: OH, O(−), ORa and ORb, NHRa and NHRb, NRaRb and NRcRd, ORa and NHRb, ORa and NRbRc, NHRa and NRbRc; and any salt thereof.

摘要翻译： 本发明涉及具有磷酸化羟甲基的新型荧光染料，其制备方法以及其在成像技术中的应用。 荧光染料是具有以下通式I-III之一的香豆素，罗丹明或BODIPY染料：其中W = OP（O）Y1Y2或P（O）Y1Y2，其中Y1和Y2独立地表示以下任何残基：OH ，O（ - ），ORa和ORb，NHRa和NHRb，NRaRb和NRcRd，ORa和NHRb，ORa和NRbRc，NHRa和NRbRc; 及其任何盐。

公开(公告)号：US20140220588A1

公开(公告)日：2014-08-07

申请号：US13702252

申请日：2011-10-18

申请人： Stefan W. Hell ,  Shamil Nizamov ,  Gerald Donnert ,  Kirill Kolmakov ,  Heiko Schill ,  Lars Kastrup ,  Christian A. Wurm ,  Vladimir N. Belov ,  Johanna Bueckers ,  Katrin Willig

发明人： Stefan W. Hell ,  Shamil Nizamov ,  Gerald Donnert ,  Kirill Kolmakov ,  Heiko Schill ,  Lars Kastrup ,  Christian A. Wurm ,  Vladimir N. Belov ,  Johanna Bueckers ,  Katrin Willig

IPC分类号： G01N33/52

CPC分类号： G01N33/52 ,  C09B11/24 ,  C09B57/02 ,  C09B57/10 ,  G01N33/533 ,  G01N33/582 ,  G01N2458/00

摘要： The invention relates to novel fluorescent dyes with phosphorylated hydroxymethyl groups, a method for preparing the same as well as to their use in imaging techniques. The fluorescent dyes are coumarin, rhodamine or BODIPY dyes having of one of the following general formulae I-III: wherein W=OP(O) Y1Y2 or P(O) Y1Y2, where Y1 and Y2 independently denote any of the following residues: OH, O(−), ORa and ORb, NHRa and NHRb, NRaRb and NRcRd, ORa and NHRb, ORa and NRbRc, NHRa and NRbRc; and any salt thereof.

摘要翻译： 本发明涉及具有磷酸化羟甲基的新型荧光染料，其制备方法以及其在成像技术中的应用。 荧光染料是具有以下通式I-III之一的香豆素，罗丹明或BODIPY染料：其中W = OP（O）Y1Y2或P（O）Y1Y2，其中Y1和Y2独立地表示以下任何残基：OH ，O（ - ），ORa和ORb，NHRa和NHRb，NRaRb和NRcRd，ORa和NHRb，ORa和NRbRc，NHRa和NRbRc; 及其任何盐。

公开(公告)号：US08755116B2

公开(公告)日：2014-06-17

申请号：US12628408

申请日：2009-12-01

申请人： Lars Kastrup ,  Volker Westphal

发明人： Lars Kastrup ,  Volker Westphal

IPC分类号： G02B5/30 ,  G02B27/00 ,  G02B27/28

CPC分类号： G02B21/0076 ,  G01N21/6458

摘要： In an optical assembly having a light source which provides two optically different light components with essentially planar wavefronts on an optical axis, wherein the light components differ at least in their wavelength; in the case of an objective lens which projects the two optically different light components into a projection space; and in the case of an optical component which is arranged on the optical axis and has an plane through which the wavefronts of the two light components pass and in which at least two different areas of the optical component with different dispersion behaviors n(λ) abut against one another in the lateral direction with respect to the optical axis; the optical component causes phase shifts of the wavefronts of the two light components, wherein the phase shift of the wavefronts of the one light component differs by at least one quarter of the wavelength of that light component between the two different areas, and wherein the phase shift of the wavefronts of the other light component does not differ between the two different areas, such that an intensity distribution of the one light component in the projection space by interference with itself differs from an intensity distribution of the other light component in the projection space.

摘要翻译： 在具有光源的光学组件中，所述光源在光轴上提供具有基本上平面的波前的两个光学不同的光分量，其中所述光分量至少在其波长上不同; 在将两个光学不同的光分量投影到投影空间中的物镜的情况下; 并且在配置在光轴上并具有两个光分量的波前通过的平面的光学部件的情况下，并且其中具有不同分散行为n（λ）的光学部件的至少两个不同区域邻接 在相对于光轴的横向上彼此相对; 光学部件引起两个光分量的波前的相移，其中一个光分量的波前的相移相差两个不同区域之间的该光分量的波长的至少四分之一，并且其中相位 另外的光分量的波前的偏移在两个不同的区域之间没有差别，使得投影空间中的一个光分量的强度分布与其自身的干涉不同于投射空间中的另一个光分量的强度分布 。