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    PRODUCTION OF N- AND O-SIALYLATED TNFRII-FC FUSION PROTEIN IN YEAST
    1.
    发明申请
    PRODUCTION OF N- AND O-SIALYLATED TNFRII-FC FUSION PROTEIN IN YEAST 审中-公开
    在YEAST中生产N-和O-齐聚的TNFRII-FC融合蛋白

    公开(公告)号：US20130330340A1

    公开(公告)日：2013-12-12

    申请号：US13985130

    申请日：2012-02-20

    申请人： Stephen R. Hamilton ,  W. James Cook ,  Sujatha Gomathinayagam

    发明人： Stephen R. Hamilton ,  W. James Cook ,  Sujatha Gomathinayagam

    IPC分类号： C07K14/705 ,  C07K16/46

    CPC分类号： C07K14/70578 ,  C07K14/7151 ,  C07K16/46 ,  C07K2319/30 ,  C12N9/1051 ,  C12N9/2402 ,  C12Y204/01101 ,  C12Y302/01113

    摘要： Production of recombinant Tumor Necrosis Factor Receptor fused to the Fc region of an antibody (TNFRII-Fc fragment fusion protein) in a glycoengineered yeast strain that is capable of producing sialylated N-glycans and O-glycans is described. Compositions of TNFRII-Fc fragment fusion protein comprising dystroglycan type O-glycans and sialylated N- and O-glycans with only terminal N-acetylneuraminic acid (NANA) residues in an α2,6-linkage are provided. In particular aspects, methods are provided for modulating the in vivo pharmacokinetics of the TNFRII-Fc fragment fusion protein by altering the O-glycan structure on the molecule.

    摘要翻译： 描述了能够产生唾液酸化N-聚糖和O-聚糖的糖工程化酵母菌株中与抗体Fc区（TNFRII-Fc片段融合蛋白）融合的重组肿瘤坏死因子受体的产生。 提供包含赖氨酸聚糖型O-聚糖和唾液酸化N-和O-聚糖的TNFRII-Fc片段融合蛋白的组合物，其仅具有α2,6键中的末端N-乙酰神经氨酸（NANA）残基。 在具体方面，提供了通过改变分子上的O-聚糖结构来调节TNFRII-Fc片段融合蛋白的体内药代动力学的方法。

    VECTORS AND YEAST STRAINS FOR PROTEIN PRODUCTION
    4.
    发明申请
    VECTORS AND YEAST STRAINS FOR PROTEIN PRODUCTION 审中-公开
    用于蛋白质生产的载体和YEAST菌株

    公开(公告)号：US20110312032A1

    公开(公告)日：2011-12-22

    申请号：US13207848

    申请日：2011-08-11

    申请人： Byung-Kwon Choi ,  Piotr Bobrowicz ,  W. James Cook ,  Elena E. Brevnova

    发明人： Byung-Kwon Choi ,  Piotr Bobrowicz ,  W. James Cook ,  Elena E. Brevnova

    IPC分类号： C12P21/00 ,  C12N9/00 ,  C12N1/19

    CPC分类号： C12N9/90 ,  C07K14/4725 ,  C12N9/1051 ,  C12N15/815 ,  C12N15/85 ,  C12N2800/102 ,  C12P21/005

    摘要： Lower eukaryote host cells in which the function of at least one endogenous gene encoding a chaperone protein, such as a Protein Disulphide Isomerase (PDI), has been reduced or eliminated and at least one mammalian homolog of the chaperone protein is expressed are described. In particular aspects, the host cells further include a deletion or disruption of one or more O-protein mannosyltransferase genes, and/or overexpression of an endogenous or exogenous Ca2+ATPase. These host cells are useful for producing recombinant glycoproteins in large amounts and for producing recombinant glycoproteins that have reduced O-glycosylation.

    摘要翻译： 描述了其中至少一个编码分子伴侣蛋白（例如蛋白质二硫键异构酶（PDI））的内源基因的功能已经被还原或消除并且伴侣蛋白的至少一个哺乳动物同系物被表达的低等真核生物宿主细胞。 在特定方面，宿主细胞还包括一个或多个O-蛋白质甘露糖基转移酶基因的缺失或破坏，和/或内源或外源Ca2 + ATP酶的过度表达。 这些宿主细胞可用于大量生产重组糖蛋白和用于产生具有降低的O-糖基化的重组糖蛋白。

    PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION
    5.
    发明申请
    PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION 有权
    生产具有减少的O-糖苷化的糖蛋白

    公开(公告)号：US20090170159A1

    公开(公告)日：2009-07-02

    申请号：US12084591

    申请日：2006-11-10

    申请人： Piotr Bobrowicz ,  W. James Cook ,  Warren Kett

    发明人： Piotr Bobrowicz ,  W. James Cook ,  Warren Kett

    IPC分类号： C12P21/00

    CPC分类号： C07K16/32 ,  C07K2317/41 ,  C12P21/005

    摘要： A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of an inhibitor of Pmt-mediated O-linked glycosylation and/or in the presence of one or more α-1,2-mannosidases.

    摘要翻译： 描述了一种用于生产具有减少量的O-连接糖基化的蛋白质组合物的方法。 该方法包括在存在Pmt介导的O-连接糖基化抑制剂和/或在一种或多种α-1,2-甘露糖苷酶存在下培养的细胞中产生蛋白质。

    PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION
    7.
    发明申请
    PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION 有权
    生产具有减少的O-糖苷化的糖蛋白

    公开(公告)号：US20120322101A1

    公开(公告)日：2012-12-20

    申请号：US13530768

    申请日：2012-06-22

    申请人： Piotr Bobrowicz ,  W. James Cook ,  Warren Kett

    发明人： Piotr Bobrowicz ,  W. James Cook ,  Warren Kett

    IPC分类号： C12P21/00

    CPC分类号： C07K16/32 ,  C07K2317/41 ,  C12P21/005

    摘要： A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of an inhibitor of Pmt-mediated O-linked glycosylation and/or in the presence of one or more α-1,2-mannosidases.

    摘要翻译： 描述了一种用于生产具有减少量的O-连接糖基化的蛋白质组合物的方法。 该方法包括在存在Pmt介导的O-连接糖基化的抑制剂和/或在一种或多种α-1,2-甘露糖苷酶的存在下培养的细胞中产生蛋白质。

    VECTORS AND YEAST STRAINS FOR PROTEIN PRODUCTION
    10.
    发明申请
    VECTORS AND YEAST STRAINS FOR PROTEIN PRODUCTION 审中-公开
    用于蛋白质生产的载体和YEAST菌株

    公开(公告)号：US20100311122A1

    公开(公告)日：2010-12-09

    申请号：US12863468

    申请日：2009-02-09

    申请人： Byung-Kwon Choi ,  Piotr Bobrowicz ,  W. James Cook

    发明人： Byung-Kwon Choi ,  Piotr Bobrowicz ,  W. James Cook

    IPC分类号： C12P21/00 ,  C12N1/19 ,  C12N9/00

    CPC分类号： C12N9/90 ,  C07K14/4725 ,  C12N9/1051 ,  C12N15/815 ,  C12N15/85 ,  C12N2800/102 ,  C12P21/005

    摘要： Lower eukaryote host cells in which the function of at least one endogenous gene encoding a chaperone protein, such as a Protein Disulphide Isomerase (PDI), has been reduced or eliminated and at least one mammalian homolog of the chaperone protein is expressed are described. In particular aspects, the host cells further include a deletion or disruption of one or more O-protein mannosyltransferase genes, and/or overexpression of an endogenous or exogenous Ca2+ ATPase. These host cells are useful for producing recombinant glycoproteins in large amounts and for producing recombinant glycoproteins that have reduced O-glycosylation.

    摘要翻译： 描述了其中至少一个编码分子伴侣蛋白（例如蛋白质二硫键异构酶（PDI））的内源基因的功能已经被还原或消除并且伴侣蛋白的至少一个哺乳动物同系物被表达的低等真核生物宿主细胞。 在特定方面，宿主细胞还包括一个或多个O-蛋白质甘露糖基转移酶基因的缺失或破坏，和/或内源或外源Ca2 + ATP酶的过度表达。 这些宿主细胞可用于大量生产重组糖蛋白和用于产生具有降低的O-糖基化的重组糖蛋白。