Modified actinomycin-based nucleic acid stains and methods of their use
    7.
    发明授权
    Modified actinomycin-based nucleic acid stains and methods of their use 有权
    改良的基于放线菌素的核酸污渍及其使用方法

    公开(公告)号:US08653261B2

    公开(公告)日:2014-02-18

    申请号:US13126150

    申请日:2009-10-26

    IPC分类号: C07D265/38

    CPC分类号: C07D265/38

    摘要: Actinomysin-based near IR emitting compounds and methods of their use as nucleic acid stains are provided. The actinomysin-based near IR emitting compounds have the structure: wherein R is H or NH2; R1, R2, R3, and R4 are independently a moiety comprising 1-30 atoms selected from H, O, C, and N, wherein the atoms are in a linear, branched, or cyclic configuration; R3 and/or R4 comprise a quaternary nitrogen atom; and R5 is H, F, or Cl.

    摘要翻译: 提供了基于放射性肌动蛋白的近红外发射化合物及其用作核酸污渍的方法。 基于放电肌注蛋白的近红外发射化合物具有以下结构:其中R是H或NH 2; R1,R2,R3和R4独立地是包含1-30个选自H,O,C和N的原子的部分,其中原子是直链,支链或环状构型; R3和/或R4包含季氮原子; R5为H,F或Cl。

    Acoustic Cytometry Methods and Protocols
    9.
    发明申请
    Acoustic Cytometry Methods and Protocols 审中-公开
    声学细胞计数法和方案

    公开(公告)号:US20140147860A1

    公开(公告)日:2014-05-29

    申请号:US14129777

    申请日:2012-06-27

    IPC分类号: G01N33/569

    摘要: Various embodiments disclosed herein comprise acoustic cytometry based methods, kits, computer software methods and systems to analyze a variety of bioparticles. In one embodiment, a method for analyzing bioparticles comprises: acoustically focusing one or more bioparticles through an interrogation zone; optically exciting the one or more bioparticles in the interrogation zone with an excitation source; detecting an optical signal from the bioparticles; and analyzing the optical signal to characterize at least one quality or quantity parameter of the bioparticles. Properties of biomolecules that may be analyzed include but are not limited to cell proliferation analysis, live/dead cell discrimination, cell cycle analysis, basic phenotyping, immunophenotyping, rare-event detection, apoptosis, phagocytosis, pinocytosis, detection of phosphoproteins, detection of one or more cellular markers, detection of one or more intracellular marker, detection of cancer cells, detection of pathological markers on a cell, microbial cell analysis and/or picophytoplankton analysis.

    摘要翻译: 本文公开的各种实施方案包括基于声学细胞计算的方法,试剂盒,计算机软件方法和用于分析各种生物颗粒的系统。 在一个实施方案中,用于分析生物颗粒的方法包括:通过询问区将一种或多种生物颗粒声学聚焦; 用激发源光学激发询问区中的一个或多个生物颗粒; 从生物颗粒检测光信号; 以及分析所述光学信号以表征所述生物颗粒的至少一个质量或数量参数。 可以分析的生物分子的性质包括但不限于细胞增殖分析,活/死细胞鉴别,细胞周期分析,基本表型,免疫分型,罕见事件检测,细胞凋亡,吞噬作用,胞饮作用,磷蛋白检测,检测一种 或更多的细胞标志物,一种或多种细胞内标志物的检测,癌细胞的检测,细胞上的病理标记的检测,微生物细胞分析和/或浮游生物分析。