公开(公告)号：US08629255B2

公开(公告)日：2014-01-14

申请号：US13112641

申请日：2011-05-20

申请人： Steven Brown ,  Adam Guss ,  Shihui Yang ,  Tatiana Karpinets ,  Lee Lynd ,  Xiongjun Shao

发明人： Steven Brown ,  Adam Guss ,  Shihui Yang ,  Tatiana Karpinets ,  Lee Lynd ,  Xiongjun Shao

IPC分类号： C07H21/00 ,  C12P7/02 ,  C12N1/00 ,  C12N15/75 ,  C12N1/15

CPC分类号： C12N9/0006 ,  C12P7/10 ,  Y02E50/16

摘要： The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.

摘要翻译： 本发明提供了分离的核酸分子，其编码突变乙醛-CoA /醇脱氢酶或突变型醇脱氢酶并赋予对乙醇增强的耐受性。 本发明还提供相关表达载体，具有增强的对乙醇的耐受性的遗传工程微生物，以及基于生物质材料的发酵制备和使用这些基因修饰的微生物生产生物燃料的方法。

公开(公告)号：US20110287499A1

公开(公告)日：2011-11-24

申请号：US13112641

申请日：2011-05-20

申请人： Steven Brown ,  Adam Guss ,  Shihui Yang ,  Tatiana Karpinets ,  Lee Lynd ,  Xiongjun Shao

发明人： Steven Brown ,  Adam Guss ,  Shihui Yang ,  Tatiana Karpinets ,  Lee Lynd ,  Xiongjun Shao

IPC分类号： C12P7/02 ,  C12N15/75 ,  C12N1/00 ,  C12N1/15 ,  C12N15/80 ,  C12N1/21 ,  C12N15/81 ,  C07H21/00 ,  C12N15/74

CPC分类号： C12N9/0006 ,  C12P7/10 ,  Y02E50/16

摘要： The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.

摘要翻译： 本发明提供了分离的核酸分子，其编码突变乙醛-CoA /醇脱氢酶或突变型醇脱氢酶并赋予对乙醇的增强的耐受性。 本发明还提供相关表达载体，具有增强的对乙醇的耐受性的遗传工程微生物，以及基于生物质材料的发酵制备和使用这些基因修饰的微生物生产生物燃料的方法。

公开(公告)号：US20060014260A1

公开(公告)日：2006-01-19

申请号：US11124941

申请日：2005-05-09

申请人： Zhiliang Fan ,  Xiongjun Shao ,  Lee Lynd

发明人： Zhiliang Fan ,  Xiongjun Shao ,  Lee Lynd

IPC分类号： C12P7/06

CPC分类号： C12P7/10 ,  C12M21/12 ,  C12M27/02 ,  C12M45/02 ,  Y02E50/16 ,  Y02E50/17

摘要： A semi-continuous simultaneous saccharification and fermentation (SSF) process for the bioconversion of cellulose into ethanol and other organic chemicals is disclosed. The process provides for substantially higher substrate conversion at a given enzyme loading (or alternatively a lower enzyme loading used to achieve the same conversion) in a reactor operated according to a semi-continuous feeding protocol.

摘要翻译： 公开了一种将纤维素生物转化成乙醇和其他有机化学品的半连续同时糖化和发酵（SSF）方法。 在根据半连续进料方案操作的反应器中，该方法提供在给定酶负载（或替代地，用于实现相同转化的较低酶负载）下的基本上更高的底物转化。

公开(公告)号：US09994835B2

公开(公告)日：2018-06-12

申请号：US12992001

申请日：2009-05-11

申请人： John McBride ,  Lee Lynd ,  Kristen Deleault

发明人： John McBride ,  Lee Lynd ,  Kristen Deleault

IPC分类号： C12N9/42 ,  C12P7/10

CPC分类号： C12N9/2437 ,  C07K2319/035 ,  C12N9/2445 ,  C12P7/10 ,  C12Y302/01004 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16

摘要： The present invention is directed to the construction of prototrophic, cellulo lytic strains of Saccharomyces cerevisiae with tethered and secreted cellulases and selection-based improvement of growth on cellulose by these strains. In some embodiments, host cells of the invention are able to produce ethanol using crystalline cellulose as a sole carbon source.

公开(公告)号：US20110256601A1

公开(公告)日：2011-10-20

申请号：US12808764

申请日：2008-12-17

申请人： Arthur Josephus Shaw, IV ,  Lee Lynd ,  David A. Hogsett

发明人： Arthur Josephus Shaw, IV ,  Lee Lynd ,  David A. Hogsett

IPC分类号： C12P7/10 ,  C12N1/19 ,  C12N9/02 ,  C12P7/06 ,  C12N1/20 ,  C07H21/04 ,  C12N1/21 ,  C12N15/63

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0067 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

摘要： Bacteria consume a variety of biomass-derived substrates and produce ethanol. Hydrogenase genes have been inactivated m Thermoanaerobacterium saccharolyticum to generate mutant strains with reduced hydrogenase activities. One such mutant strain with both the ldh and hydtrA genes inactivated shows a significant increase in ethanol production. Manipulation of hydrogenase activities provides a new approach for enhancing substrate utilization and ethanol production by biomass-fermenting microorganisms.

摘要翻译： 细菌消耗各种生物质来源的底物并产生乙醇。 氢解酶基因已被灭活，从而产生具有降低的氢化酶活性的突变菌株。 一个这样的具有ldh和hydtrA基因失活的这种突变菌株显示出乙醇生产的显着增加。 氢化酶活性的操作提供了生物质发酵微生物增强底物利用和乙醇生产的新方法。

公开(公告)号：US20110189744A1

公开(公告)日：2011-08-04

申请号：US12992001

申请日：2009-05-11

申请人： John Mcbride ,  Lee Lynd ,  Kristen Deleault

发明人： John Mcbride ,  Lee Lynd ,  Kristen Deleault

IPC分类号： C12P7/10 ,  C12N1/19 ,  C12N1/22

CPC分类号： C12N9/2437 ,  C07K2319/035 ,  C12N9/2445 ,  C12P7/10 ,  C12Y302/01004 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16

摘要： The present invention is directed to the construction of prototrophic, cellulo lytic strains of Saccharomyces cerevisiae with tethered and secreted cellulases and selection-based improvement of growth on cellulose by these strains. In some embodiments, host cells of the invention are able to produce ethanol using crystalline cellulose as a sole carbon source.

摘要翻译： 本发明涉及利用拴系和分泌的纤维素酶构建酿酒酵母的营养型纤维素裂解菌株，并且通过这些菌株基于选择性改善纤维素的生长。 在一些实施方案中，本发明的宿主细胞能够使用结晶纤维素作为唯一的碳源来生产乙醇。