公开(公告)号：US20070291265A1

公开(公告)日：2007-12-20

申请号：US11735206

申请日：2007-04-13

申请人： Steven Holman ,  Bin Guo ,  W. Wilson ,  Joseph Fanguy

发明人： Steven Holman ,  Bin Guo ,  W. Wilson ,  Joseph Fanguy

IPC分类号： G01J3/42 ,  G06F19/00

CPC分类号： G01J3/10 ,  G01J3/02 ,  G01J3/0218 ,  G01N21/05 ,  G01N21/532 ,  G01N2021/513 ,  G01N2201/0826

摘要： This invention relates to a fiber optic apparatus for simultaneously measuring the scattering and concentration signals of macromolecules in a flow cell 3. The apparatus is based on the delivery/focusing of both a laser and ultraviolet light source to the same physical position in a low volume flow cell 4, via a bifurcated optical fiber 3. This configuration allows the light scattering and concentration signal changes associated with a macromolecular solution passing through the flow channel to be measured simultaneously. This invention also relates to a method that uses the optical apparatus 10 to determine properties of a macromolecular solution such as the ideal crystallization and/or formulation conditions (via B22) for a given protein solution.

摘要翻译： 本发明涉及一种用于同时测量流动池3中大分子的散射和浓度信号的光纤设备。 该装置基于通过分叉光纤3将激光和紫外光源两者的传送/聚焦到低体积流动池4中相同的物理位置。 该配置允许与通过要同时测量的流动通道的大分子溶液相关联的光散射和浓度信号变化。 本发明还涉及一种使用光学装置10来确定对于给定蛋白质溶液的大分子溶液的性质，例如理想结晶和/或配制条件（通过B 22）的方法。