公开(公告)号：US20090162346A1

公开(公告)日：2009-06-25

申请号：US12231877

申请日：2008-09-04

申请人： Steven J. Schrodi ,  Ann B. Begovich

发明人： Steven J. Schrodi ,  Ann B. Begovich

IPC分类号： A61K39/395 ,  C12Q1/68 ,  A61P19/02

CPC分类号： C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/172

摘要： The present invention provides compositions and methods based on genetic polymorphisms that are associated with autoimmune disease, particularly rheumatoid arthritis. For example, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by these nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and variant proteins, and methods of using the nucleic acid molecules and proteins as well as methods of using reagents for their detection.

摘要翻译： 本发明提供了基于与自身免疫疾病特别是类风湿性关节炎有关的遗传多态性的组合物和方法。 例如，本发明涉及含有多态性的核酸分子，由这些核酸分子编码的变体蛋白，用于检测多态型核酸分子和变体蛋白的试剂，以及使用核酸分子和蛋白质的方法以及方法 使用试剂进行检测。

公开(公告)号：US5541065A

公开(公告)日：1996-07-30

申请号：US195615

申请日：1994-02-14

申请人： Henry A. Erlich ,  Glenn T. Horn ,  Teodorica Bugawan ,  Ann B. Begovich

发明人： Henry A. Erlich ,  Glenn T. Horn ,  Teodorica Bugawan ,  Ann B. Begovich

IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K49/00 ,  C07K14/74 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566 ,  C07H21/04

CPC分类号： C12Q1/6881 ,  C07K14/70539 ,  C12Q1/6811 ,  C12Q2600/156

摘要： A process for determining the genotype of an individual with respect to the alleles at the HLA DP locus involves obtaining a sample of nucleic acid from the individual, and hybridizing the nucleic acids with a panel of probes specific for variant segments of DPalpha and DPbeta genes. Because the variation between DPbeta alleles is highly dispersed throughout the second exon of the DPbeta gene, the discovery of many different DPbeta alleles makes the process far more discriminating and informative than cellular, RFLP, or serological methods. The process can also be carried out on amplified nucleic acid produced by the polymerase chain reaction using primers specific for the second exon of the DPalpha and DPbeta genes. HLA DP DNA typing methods are useful in the prevention of graft rejection and host versus graft disease, in determining susceptibility to autoimmune diseases, in providing evidence concerning the derivation from an individual of forensic samples, and in paternity testing.

摘要翻译： 用于确定个体相对于HLA DP位点处的等位基因的基因型的方法包括从个体获得核酸样品，并将核酸与DPalpha和DPbeta基因的变体片段特异性的一组探针杂交。 因为DPbeta等位基因之间的差异在DPbeta基因的第二个外显子中高度分散，许多不同的DPbeta等位基因的发现使得该过程比细胞，RFLP或血清学方法更具歧视性和信息性。 该方法还可以通过聚合酶链式反应产生的扩增核酸进行，其使用对DPalpha和DPbeta基因的第二外显子特异的引物。 HLA DP DNA分型方法可用于预防移植排斥反应和宿主与移植物疾病，确定对自身免疫性疾病的易感性，提供有关法医样本个体的推导和亲子鉴定的证据。

公开(公告)号：US5310893A

公开(公告)日：1994-05-10

申请号：US347506

申请日：1989-05-04

申请人： Henry A. Erlich ,  Glenn T. Horn ,  Teodorica Bugawan ,  Ann B. Begovich

发明人： Henry A. Erlich ,  Glenn T. Horn ,  Teodorica Bugawan ,  Ann B. Begovich

IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K49/00 ,  C07K14/74 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566 ,  C07H21/04 ,  C12N15/00

CPC分类号： C12Q1/6881 ,  C07K14/70539 ,  C12Q1/6811 ,  C12Q2600/156

摘要： A process for determining the genotype of an individual with respect to the alleles at the HLA DP locus involves obtaining a sample of nucleic acid from the individual, and hybridizing the nucleic acids with a panel of probes specific for variant segments of DPalpha and DPbeta genes. Because the variation between DPbeta alleles is highly dispersed throughout the second exon of the DPbeta gene, the discovery of many different DPbeta alleles makes the process far more discriminating and informative than cellular, RFLP, or serological methods. The process can also be carried out on amplified nucleic acid produced by the polymerase chain reaction using primers specific for the second exon of the DPalpha and DPbeta genes. HLA DP DNA typing methods are useful in the prevention of graft rejection and host versus graft disease, in determining susceptibility to autoimmune diseases, in providing evidence concerning the derivation from an individual of forensic samples, and in paternity testing.

摘要翻译： 用于确定个体相对于HLA DP位点处的等位基因的基因型的方法包括从个体获得核酸样品，并将核酸与DPalpha和DPbeta基因的变体片段特异性的一组探针杂交。 因为DPbeta等位基因之间的差异在DPbeta基因的第二个外显子中高度分散，许多不同的DPbeta等位基因的发现使得该过程比细胞，RFLP或血清学方法更具歧视性和信息性。 该方法还可以通过聚合酶链式反应产生的扩增核酸进行，其使用对DPalpha和DPbeta基因的第二外显子特异的引物。 HLA DP DNA分型方法可用于预防移植排斥反应和宿主与移植物疾病，确定对自身免疫性疾病的易感性，提供有关法医样本个体的推导和亲子鉴定的证据。