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    Assay systems and methods for detecting molecules that interact with membrane channels
    3.
    发明授权
    Assay systems and methods for detecting molecules that interact with membrane channels 有权
    用于检测与膜通道相互作用的分子的测定系统和方法

    公开(公告)号：US07927820B2

    公开(公告)日：2011-04-19

    申请号：US10894836

    申请日：2004-07-20

    申请人： Sui-Po Zhang ,  Ellen E. Codd

    发明人： Sui-Po Zhang ,  Ellen E. Codd

    IPC分类号： G01N33/53

    CPC分类号： G01N33/542 ,  G01N33/6872 ,  G01N2500/10

    摘要： The invention provides an assay system for detecting a molecule that interacts with a membrane channel, the assay system including cell membranes including one or more membrane channels; support bodies including scintillant and a coupling agent that associates with the cell membrane; a ligand that is selected to bind the membrane channel, the ligand including a scintillant-activating label. According to the invention, association of the support bodies with the cell membrane and binding of the ligand to the membrane channel results in emission from the scintillant of the support bodies, and, in the presence of a test molecule that interacts with the membrane channel, the emission from the scintillant of the support bodies changes. Methods of identifying a molecule that interacts with a cell membrane channel are also described.

    摘要翻译： 本发明提供了一种用于检测与膜通道相互作用的分子的测定系统，该测定系统包括包含一个或多个膜通道的细胞膜; 支持体，包括闪烁体和与细胞膜缔合的偶联剂; 被选择以结合膜通道的配体，该配体包括闪烁体激活标记。 根据本发明，支持体与细胞膜的结合和配体与膜通道的结合导致来自支持体的闪烁体的发射，并且在与膜通道相互作用的测试分子的存在下， 来自支撑体的闪烁体的发射变化。 还描述了鉴定与细胞膜通道相互作用的分子的方法。

    Ligand binding assays for vanilloid receptors
    5.
    发明授权
    Ligand binding assays for vanilloid receptors 有权
    香草素受体的配体结合测定

    公开(公告)号：US06780650B2

    公开(公告)日：2004-08-24

    申请号：US09975650

    申请日：2001-10-11

    申请人： Sui-Po Zhang ,  Ellen E. Codd

    发明人： Sui-Po Zhang ,  Ellen E. Codd

    IPC分类号： G01N30566

    CPC分类号： G01N33/566 ,  G01N33/5306 ,  G01N2333/705 ,  G01N2500/04

    摘要： The present invention describes an assay in which pH alters RTX affinity to VR1 receptors. The RTX affinity was increased with increasing pH from 4.1 to 8.6. Both the RTX affinity and apparent number of RTX binding sites were decreased with increasing pH from 8.6 to 10.6. The high pH may be causing the cell membranes to denature. These pH conditions (pH 8.0 to pH 9.0) provide a high signal to noise ratio, give a more robust assay and require use of less experimental materials.

    摘要翻译： 本发明描述了其中pH改变对VR1受体的RTX亲和力的测定。 随着pH值从4.1增加到8.6，RTX亲和力增加。 RTX亲和力和RTX结合位点的表观数都随着pH值从8.6降低到10.6而降低。 高pH可能导致细胞膜变性。 这些pH条件（pH8.0至pH9.0）提供高的信噪比，给出更强的测定，并且需要使用较少的实验材料。