公开(公告)号：USPP16566P3

公开(公告)日：2006-05-23

申请号：US10401691

申请日：2003-03-31

申请人： Suman Preet Singh Khanuja ,  Ajit Kumar Shasany ,  Usha Yadav ,  Sunita Dhawan ,  Mahendra Pandurang Darokar ,  Janak Raj Bahl ,  Soni Gupta ,  Sweta Pandey ,  Anil Kumar Singh ,  Ravi Prakash Bansal ,  Raj Kishori Lal ,  Om Parkash Dhawan ,  Ali Arif Naqvi ,  Alok Kaira ,  Alok Krishna ,  Virendra Kumar Singh Tomar ,  Anil Kumar Singh

发明人： Suman Preet Singh Khanuja ,  Ajit Kumar Shasany ,  Usha Yadav ,  Sunita Dhawan ,  Mahendra Pandurang Darokar ,  Janak Raj Bahl ,  Soni Gupta ,  Sweta Pandey ,  Anil Kumar Singh ,  Ravi Prakash Bansal ,  Raj Kishori Lal ,  Om Parkash Dhawan ,  Ali Arif Naqvi ,  Alok Kaira ,  Alok Krishna ,  Virendra Kumar Singh Tomar ,  Anil Kumar Singh

IPC分类号： A01H5/00

CPC分类号： C12Q1/6895 ,  A01H1/04 ,  A01H5/02 ,  A01H5/12 ,  C12Q2600/13 ,  C12Q2600/156

摘要： The present invention was related to the development of a novel, distinct high yielding plant with rapid regeneration ability obtained through screening of the somaclones in a methodical way for fast regeneration in the tissue culture stage itself which was achieved by inventing the plant ‘Kushal’. The plant yield higher herbage with corresponding high essential oil when evaluated with available superior varieties of mint in late planting condition during April when the fields are vacated after the harvest of Rabi crop like wheat, chickpea, coriander etc. Further the suckers required for commercial vegetative planting can be produced even in low land condition as the plant is reasonably tolerant to water logging compared to the best check ‘Kosi’.

摘要翻译： 本发明涉及一种通过以组织培养阶段本身的快速再生的有条理的方式筛选松果体而获得的具有快速再生能力的新型不同高产植物的发展，该发明是通过发明植物“Kushal”而实现的。 在4月当田间在Rabi作物如麦子，鹰嘴豆，香菜等收获后腾出田间时，用较高品种的薄荷素在晚期种植条件下，用相当高的精油进行植物产量较高的草药。进一步的营养需要的吮吸者 即使在低土地条件下种植也可以生产，因为植物相对于最佳支架“Kosi”而言相对地耐水测井。

公开(公告)号：US06893667B2

公开(公告)日：2005-05-17

申请号：US10104062

申请日：2002-03-22

申请人： Rajender Singh Sangwan ,  Neelam Singh Sangwan ,  Bali Ram Tyagi ,  Avdhesh Kumar Srivastava ,  Usha Yadav

发明人： Rajender Singh Sangwan ,  Neelam Singh Sangwan ,  Bali Ram Tyagi ,  Avdhesh Kumar Srivastava ,  Usha Yadav

IPC分类号： C12Q1/28 ,  C12Q1/44 ,  C12Q1/52 ,  A61K35/78 ,  A01N37/18

CPC分类号： C12Q1/52 ,  C12Q1/28 ,  C12Q1/44 ,  G01N2550/00

摘要： The present invention relates to a method for profiling of protein extract from hyper-acidic aerial parts of plant Scented Geranium (Pelargonium sp.), said method comprising steps of selecting effective extraction medium, with extraction medium consisting of 0.2 M sodium carbonate increasing percentage extract by about 600%, selecting effective medium to determine polypeptide pattern in said extract, with polypeptide pattern medium consisting of Sodium Carbonate ranging between 0.2 to 0.4 M, and 0.2 M Sodium Carbonate along with 50% dimethylsulfoxide, selecting effective media to determine enzyme multimolecular forms in said extract, with enzyme multimolecular forms media consisting of 0.2 M Tris-HCl buffer at pH 7.5, and optionally 50% dimethylsulfoxide; Sodium Carbonate at concentration ranging between 0.2 to 0.4 M and optionally 50% dimethylsulfoxide, and using biochemical and biophysical techniques comprising gel electrophoresis, spectrophotometry, centrifugation, protein estimation in said method to determine quantitative, qualitative, structural and functional facets of the proteins from said extract, and extraction compositions thereof.

摘要翻译： 本发明涉及一种用于从植物香味天竺葵（天竺葵属）的高酸性地上部分分析蛋白质提取物的方法，所述方法包括以下步骤：选择有效的提取介质，用0.2M碳酸钠提取百分比提取物 选择有效培养基以确定所述提取物中的多肽模式，多肽模式培养基由碳酸钠组成，范围在0.2至0.4M之间，0.2M碳酸钠与50％二甲基亚砜组成，选择有效培养基以确定酶多分子形式 在所述提取物中，用酶多分子形式的培养基由pH7.5的0.2M Tris-HCl缓冲液和任选的50％二甲基亚砜组成; 碳酸钠的浓度范围为0.2至0.4M，任选的50％的二甲基亚砜，并且使用生物化学和生物物理技术，包括凝胶电泳，分光光度法，离心，所述方法中的蛋白质估计，以确定所述蛋白质的定量，定性，结构和功能方面 提取物及其提取组合物。