利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

5 条记录 (耗时 0.019 秒)

    Modified GAF polypeptides
    2.
    发明授权
    Modified GAF polypeptides 失效
    改良的GAF多肽

    公开(公告)号：US5571895A

    公开(公告)日：1996-11-05

    申请号：US172328

    申请日：1993-12-21

    申请人： Tsutomu Kurokawa ,  Ken-ichi Kuroshima ,  Sumie Yoshitomi ,  Chisako Seko

    发明人： Tsutomu Kurokawa ,  Ken-ichi Kuroshima ,  Sumie Yoshitomi ,  Chisako Seko

    IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K14/50 ,  C07K16/22 ,  C12N15/12 ,  C12P21/08 ,  C07K14/475

    CPC分类号： C07K16/22 ,  C07K14/50 ,  A61K38/00 ,  A61K39/00 ,  C07K2317/73

    摘要： The present invention relates to a glia activating factor (GAF) in which 33 to 49 N-terminal amino acids are deleted from the N-terminus of the GAF polypeptide of SEQ ID NO: 3, as well as an anti-GAF antibody which is highly sensitive to assay the GAF and is capable of neutralizing GAF's activity, is produced.Using the antibody, GAF and its biological activity can be assayed easily with high sensitivity. Further, the neutralizing antibody can be expected to have preventive and curative effects on diseases caused by GAF's excessive action. Furthermore, the GAF polypeptide disclosed herein is more stable to acids and heat than the conventional GAFs, therefore, it can be used as a platelet-increasing agent, etc., more advantageously than the previously known GAFs.

    摘要翻译： 本发明涉及从SEQ ID NO：3的GAF多肽的N末端缺失33〜49个N-末端氨基酸的神经胶质激活因子（GAF），以及抗GAF抗体 产生了高度灵敏的测定GAF并能够中和GAF的活性。 使用抗体，可以高灵敏度地容易地测定GAF及其生物活性。 此外，中和抗体可以预期对由GAF过度作用引起的疾病具有预防和治疗作用。 此外，本文公开的GAF多肽比常规GAF对酸和热更稳定，因此，其可以比先前已知的GAF更有利地用作血小板递增剂等。

    Human cell line showing stable expression of cytochromes P450
    3.
    发明授权
    Human cell line showing stable expression of cytochromes P450 失效
    显示细胞色素P450的稳定表达的人细胞系

    公开(公告)号：US06756229B1

    公开(公告)日：2004-06-29

    申请号：US10009158

    申请日：2001-10-25

    申请人： Masayoshi Nanba ,  Satoru Asahi ,  Sumie Yoshitomi ,  Keiko Ikemoto

    发明人： Masayoshi Nanba ,  Satoru Asahi ,  Sumie Yoshitomi ,  Keiko Ikemoto

    IPC分类号： C12N508

    CPC分类号： C12Q1/26 ,  G01N2333/795 ,  G01N2333/80 ,  G01N2333/90245

    摘要： This invention relates to cell lines that are obtained using cultured cell lines derived from human liver as a host and that stably express a number of human cytochromes P450. The human liver-derived cultured cell lines of the present invention are useful in, for example, analyzing an enzyme participating in the metabolism of xenobiotics or endogenous substrates, because of their stable expression of human cytochromes P450 CYP1A1, 1A2, 2A6, 2B6, 2C8, 2C9, 2C9, 2D6, and 3A4.

    摘要翻译： 本发明涉及使用源自人肝脏的培养细胞系作为宿主获得并稳定表达许多人细胞色素P450的细胞系。 本发明的人肝源培养细胞系可用于例如分析参与异生物或内源底物的代谢的酶，因为它们稳定地表达人细胞色素P450 CYP1A1，1A2,2A6,2B6,2C8 ，2C9,2C9,2D6,3A4。