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1.发明授权Method for detecting methylation of promoter using restriction enzyme and DNA chip 有权使用限制酶和DNA芯片检测启动子甲基化的方法公开(公告)号：US07611841B2
公开(公告)日：2009-11-03
申请号：US10983809
申请日：2004-11-08
申请人： Sungwhan An , ChiWang Yoon , TaeJeong Oh , DaeKyoung Yoon , SunWoo Lee , MyungSoon Kim , SukKyung Woo
发明人： Sungwhan An , ChiWang Yoon , TaeJeong Oh , DaeKyoung Yoon , SunWoo Lee , MyungSoon Kim , SukKyung Woo
CPC分类号： C12Q1/6858 , C12Q2521/331 , C12Q2565/501
摘要： A method for detecting the methylation of promoters using HpaII, a methylation-sensitive restriction enzyme. In such method, DNAs, derived from clinical samples or subjects to be diagnosed, are cut with HpaII, the cut DNAs are amplified by PCR with primers capable of amplifying CpG islands, and the presence or absence of the PCR amplification products is determined using a DNA chip for methylation detection. Unlike prior approaches, the inventive method allows the methylation of gene promoters to be detected in a simple and economical manner, and thus is useful for the diagnosis of diseases such as cancer that are characterized by methylation of gene promoters.
摘要翻译： 使用甲基化敏感性限制酶HpaII检测启动子的甲基化的方法。 在这种方法中，用HpaII切割衍生自待诊断的临床样品或受试者的DNA，通过用扩增CpG岛的引物通过PCR扩增切割的DNA，并且使用以下步骤确定PCR扩增产物的存在或不存在 用于甲基化检测的DNA芯片。 与现有方法不同，本发明的方法允许以简单和经济的方式检测基因启动子的甲基化，因此可用于诊断特征为基因启动子甲基化的疾病。
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2.发明申请Methylated promoters of colon cancer-specific expression-decreased genes and use thereof 审中-公开结肠癌特异性表达的甲基化启动子减少的基因及其用途公开(公告)号：US20060068402A1
公开(公告)日：2006-03-30
申请号：US10984481
申请日：2004-11-09
申请人： Sungwhan An , ChiWang Yoon , TaeJeong Oh , DaeKyoung Yoon , SunWoo Lee , MyungSoon Kim , SukKyung Woo
发明人： Sungwhan An , ChiWang Yoon , TaeJeong Oh , DaeKyoung Yoon , SunWoo Lee , MyungSoon Kim , SukKyung Woo
CPC分类号： C12Q1/6886 , C12Q2600/154
摘要： Methylated promoters of colon cancer-specific genes and use thereof. Various disclosed aspects of the invention include methylated promoters of the colon cancer specific expression-decreased genes, microarrays for cancer diagnosis on which the methylated promoters are immobilized, and cancer diagnosis kits containing the methylated promoters. The methylated promoters of colon cancer-specific expression-decreased genes have utility for early detection of cancer and as targets for screening new drugs useful in the early treatment of cancer.
摘要翻译： 结肠癌特异性基因的甲基化启动子及其用途。 本发明的各种公开的方面包括结肠癌特异性表达减少的基因的甲基化启动子，用于其中固定有甲基化启动子的癌症诊断的微阵列，以及含有甲基化启动子的癌症诊断试剂盒。 结肠癌特异性表达降低的基因的甲基化启动子具有早期检测癌症的用途，并且作为用于筛选可用于早期治疗癌症的新药物的靶标。
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公开(公告)号：US20060063165A1
公开(公告)日：2006-03-23
申请号：US10984640
申请日：2004-11-09
申请人： Sungwhan An , ChiWang Yoon , TaeJeong Oh , DaeKyoung Yoon , SunWoo Lee , MyungSoon Kim , SukKyung Woo
发明人： Sungwhan An , ChiWang Yoon , TaeJeong Oh , DaeKyoung Yoon , SunWoo Lee , MyungSoon Kim , SukKyung Woo
CPC分类号： C12Q1/6853 , C12Q2525/155 , C12Q2521/107 , C12Q2565/501
摘要： A method for the linear amplification of RNA using a high-heel primer, in which annealing and extension are performed at the same temperature using a high-heel primer. A DNA primer rather than an RNA primer is used, to provide a highly stable linear amplification process that can easily amplify even a small amount of sample, allowing a sufficient amount of target for microarray tests to be obtained from a nanogram level of total RNA by one linear amplification process using a high-heel primer. The inventive method shows a high correlation between RNAs of the same origin regardless of RNA amounts and purification methods, and efficiently achieves linear amplification within a short time at low cost as compared to prior methodology.
摘要翻译： 使用高跟踪引物线性扩增RNA的方法，其中使用高跟引物在相同温度下进行退火和延伸。 使用DNA引物而不是RNA引物，以提供高度稳定的线性扩增方法，其可以容易地扩增甚至少量样品，从而可以从纳克级总RNA获得足够量的用于微阵列测试的靶标 一个使用高跟踪引物的线性扩增过程。 无论RNA量和纯化方法如何，本发明的方法显示出相同来源的RNA之间的高度相关性，并且与现有技术相比在低成本的时间内有效地实现了线性扩增。
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公开(公告)号：US20060063164A1
公开(公告)日：2006-03-23
申请号：US10983809
申请日：2004-11-08
申请人： Sungwhan An , ChiWang Yoon , TaeJeong Oh , DaeKyoung Yoon , SunWoo Lee , MyungSoon Kim , SukKyung Woo
发明人： Sungwhan An , ChiWang Yoon , TaeJeong Oh , DaeKyoung Yoon , SunWoo Lee , MyungSoon Kim , SukKyung Woo
CPC分类号： C12Q1/6858 , C12Q2521/331 , C12Q2565/501
摘要： A method for detecting the methylation of promoters using HpaII, a methylation-sensitive restriction enzyme. In such method, DNAs, derived from clinical samples or subjects to be diagnosed, are cut with HpaII, the cut DNAs are amplified by PCR with primers capable of amplifying CpG islands, and the presence or absence of the PCR amplification products is determined using a DNA chip for methylation detection. Unlike prior approaches, the inventive method allows the methylation of gene promoters to be detected in a simple and economical manner, and thus is useful for the diagnosis of diseases such as cancer that are characterized by methylation of gene promoters.
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