SYSTEM FOR BIOMARKER DISCOVERY
    6.
    发明申请
    SYSTEM FOR BIOMARKER DISCOVERY 审中-公开
    生物标记发现系统

    公开(公告)号:US20060211009A1

    公开(公告)日:2006-09-21

    申请号:US11277033

    申请日:2006-03-20

    IPC分类号: C12Q1/68 C07H21/04

    摘要: The present application discloses a method for discovering a methylation marker gene for the conversion of a cell comprising: (i) comparing converted and unconverted cell gene expression content to identify a gene that is present in greater abundance in the unconverted cell; (ii) treating a converted cell with a demethylating agent and comparing its gene expression content with gene expression content of an untreated converted cell to identify a gene that is present in greater abundance in the cell treated with the demethylating agent; and (iii) identifying a gene that is common to the identified genes in steps (i) and (ii), wherein the common identified gene is the methylation marker gene.

    摘要翻译: 本申请公开了一种用于发现用于转化细胞的甲基化标记基因的方法,包括:(i)比较转化和未转化的细胞基因表达含量,以鉴定未转化细胞中更丰富的基因; (ii)用去甲基化剂处理转化的细胞,并将其基因表达含量与未处理的转化细胞的基因表达含量进行比较,以鉴定在用去甲基化试剂处理的细胞中更丰富的基因; 和(iii)鉴定步骤(i)和(ii)中所鉴定的基因共有的基因,其中常见的鉴定基因是甲基化标记基因。