公开(公告)号：US5411749A

公开(公告)日：1995-05-02

申请号：US996831

申请日：1992-12-23

申请人： Susan K. Mayo ,  Reiko Namikawa ,  Hideto Kaneshima ,  Joseph M. McCune

发明人： Susan K. Mayo ,  Reiko Namikawa ,  Hideto Kaneshima ,  Joseph M. McCune

IPC分类号： A01K67/027 ,  A61K35/00 ,  A61K39/00 ,  C07K16/00 ,  C12N5/08 ,  C12N5/10 ,  C12N15/00 ,  C12N15/02 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  A61K49/00

CPC分类号： A01K67/0271 ,  C07K16/00

摘要： Human lymphoid tissue is introduced into an immunocompromised host to provide opportunities to investigate the effect of stimuli on the human immune system and obtain information and products from the stimulus. Particularly, methodology is provided for the production of human monoclonal antibodies by introducing lymphoid tissue into an immunocompromised host, stimulating the B-lymphocytes with an appropriate immunogen, harvesting the tissue and immortalizing and/or cloning the B-lymphocytes under conditions to obtain a stable supply of monoclonal antibodies.