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    DNA encoding recombinant human lymphotoxin
    1.
    发明授权
    DNA encoding recombinant human lymphotoxin 失效
    编码重组人淋巴毒素的DNA

    公开(公告)号：US5175268A

    公开(公告)日：1992-12-29

    申请号：US416657

    申请日：1989-10-03

    申请人： Susumu Iwasa ,  Tomoko Fujii ,  Ryuji Marumoto ,  Koichi Igarashi

    发明人： Susumu Iwasa ,  Tomoko Fujii ,  Ryuji Marumoto ,  Koichi Igarashi

    IPC分类号： C07K14/525 ,  C12N15/19

    CPC分类号： C07K14/525

    摘要： Lymphotoxin (LT) mutein (genetically-altered LT) is disclosed that has the following amino acid sequence, or a portion of an active portion of said protein, where 10 to 21 amino acids of LT being deleted from N-terminus and which has Pro or Phe at the N-terminus: ##STR1## Wherein R.sub.1 is Pro or Phe, R.sub.2 is a peptide chain represented by the following sequence:Ala-Gln-Thr-Ala-Arg-Gln-His-Pro-Lys-Met-His-Leu,or a portion thereof and n is 0 or 1.The LT mutein can be recovered in a higher yield and purified more efficiently under mild conditions which does not harm the LT's biological activity, than the whole LT.

    摘要翻译： 公开了具有以下氨基酸序列或所述蛋白质的活性部分的一部分的淋巴毒素（LT）突变蛋白（遗传改变的LT），其中LT的10至21个氨基酸从N末端缺失并且具有Pro 或N末端的Phe：Ala-His-Leu-Ile-Gly-Asp-Pro-Ser-Lys-Gln-Asn-Ser-Leu-Leu-Trp-Arg-Ala-Asn-Thr-Asp -Arg-Ala-Phe-Leu-Gln-Asp-Gly-Phe-Ser-Leu-Ser-Asn-Asn-Ser-Leu-Leu- Val-Pro-Thr-Ser-Gly-Ile-Tyr-Phe-Val - Tyr-Ser-Gln-Val-Val-Phe-Ser-Gly-Lys-Ala-Tyr-Ser-Pro-Lys-Ala-Thr-Ser-Ser-Pro-Leu-Tyr-Leu-Ala-His-Glu -Val-Gln-Leu-Phe-Ser-Ser-Gln-Tyr-Pro-Phe-His-Val-Pro-Leu-Leu-Ser-Ser-Gln-Lys-Met-Val-Tyr-Pro-Gly-Leu -Gln-Glu-Pro-Trp-Leu-His-Ser-Met-Tyr-His-Gly-Ala-Al-Phe-Gln-Leu-Thr-Gln-Gly-Asp-Gln-Leu-Ser-Thr-His -Thr-Asp-Gly-Ile-Pro-His-Leu-Val-Leu-Ser-Pro-Ser-Thr-Val-Phe-Phe-Gly-Ala-Phe-Ala-Leu-OH其中R1是Pro或Phe R2是由以下序列表示的肽链：Ala-Gln-Thr-Ala-Arg-Gln-His-Pro-Lys-Met-His-Leu或端口 并且n为0或1.可以比在整个LT中更高的产率回收LT突变蛋白，并且在不影响LT的生物活性的温和条件下更有效地纯化。

    Expression vector for animal cell line and use thereof
    2.
    发明授权
    Expression vector for animal cell line and use thereof 失效
    动物细胞系的表达载体及其用途

    公开(公告)号：US4935352A

    公开(公告)日：1990-06-19

    申请号：US919798

    申请日：1986-10-16

    申请人： Koichi Igarashi ,  Reiko Sasada ,  Tomoko Fujii ,  Ryuji Marumoto

    发明人： Koichi Igarashi ,  Reiko Sasada ,  Tomoko Fujii ,  Ryuji Marumoto

    IPC分类号： C07K14/485 ,  C07K14/55 ,  C07K16/00 ,  C12N15/85

    CPC分类号： C12N15/85 ,  C07K14/485 ,  C07K14/55 ,  C07K16/00 ,  C07K2319/02

    摘要： An animal cell line transformed with an expression vector for an animal cell, the expression vector which contains a DNA segment comprising:(a) a DNA sequence coding for a signal peptide of an animal cell-derived protein,(b) a second DNA sequence coding for a different protein from the signal protein joined downstream of said signal peptide encoding DNA sequence without causing any reading frame shift, and(c) a promoter DNA sequence capable of functioning in an animal cell, wherein the promoter sequence is positioned upstream of the signal peptide encoding DNA sequence, can produce glycosylated proteins advantageously as secretable proteins.

    摘要翻译： 用动物细胞表达载体转化的动物细胞系，含有DNA片段的表达载体包含：（a）编码动物细胞来源的蛋白质的信号肽的DNA序列，（b）第二DNA序列 编码来自所述信号肽编码DNA序列下游的信号蛋白的不同蛋白质，而不引起任何阅读框移动，和（c）能够在动物细胞中起作用的启动子DNA序列，其中启动子序列位于 信号肽编码DNA序列，可以有利地产生糖基化的蛋白质作为可分泌的蛋白质。

    Optical waveguide in the interior of silica glass and method of forming optical waveguide
    4.
    发明授权
    Optical waveguide in the interior of silica glass and method of forming optical waveguide 失效
    石英玻璃内部的光波导和形成光波导的方法

    公开(公告)号：US06945078B2

    公开(公告)日：2005-09-20

    申请号：US10644827

    申请日：2003-08-21

    申请人： Takeshi Fukuda ,  Shimon Ishikawa ,  Tomoko Fujii ,  Ken Sakuma ,  Hideyuki Hosoya

    发明人： Takeshi Fukuda ,  Shimon Ishikawa ,  Tomoko Fujii ,  Ken Sakuma ,  Hideyuki Hosoya

    IPC分类号： B23K26/03 ,  B23K26/06 ,  C03C23/00 ,  G02B6/13 ,  C02B37/023 ,  G02B6/10 ,  C02B37/022

    CPC分类号： G02B6/13 ,  B23K26/032 ,  B23K26/0624 ,  C03C23/0025

    摘要： When an optical waveguide is formed by focused femtosecond laser pulses in a pure silica glass to induce a refractive index increase region, a pulse width of femtosecond laser pulses are changed, a peak power of femtosecond laser pulses at the focal point is changed, or both the pulse width and the peak power are changed simultaneously. Under conditions where a pulse width of the femtosecond laser pulses is in a range of 210 to 420 fs and a peak power at the focal point is not greater than 8.7×1011 W/cm2, an optical waveguide having a mode field of 10 to 14 μm such that an aspect ratio is 1 (one) can be obtained. By doing this, it is possible to control a mode field diameter of an optical waveguide and an aspect ratio of the mode field diameter.

    摘要翻译： 当通过在纯二氧化硅玻璃中的聚焦飞秒激光脉冲形成光波导以引起折射率增加区域时，飞秒激光脉冲的脉冲宽度改变，焦点处的飞秒激光脉冲的峰值功率改变，或两者都改变 脉冲宽度和峰值功率同时变化。 在飞秒激光脉冲的脉冲宽度在210至420fs的范围内且焦点处的峰值功率不大于8.7x10 11 / cm 2的条件下， / SUP>，具有10〜14μm的模场的光波导，使得可以获得纵横比为1（1）。 通过这样做，可以控制光波导的模场直径和模场直径的纵横比。