公开(公告)号：US5443978A

公开(公告)日：1995-08-22

申请号：US82844

申请日：1993-06-25

申请人： Suzanne R. Ellenberger ,  Galen D. Peiser ,  Russell N. Bell ,  Charles E. Hussey, Jr. ,  Donna M. Shattuck-Eidens ,  Bradley D. Swedlund

发明人： Suzanne R. Ellenberger ,  Galen D. Peiser ,  Russell N. Bell ,  Charles E. Hussey, Jr. ,  Donna M. Shattuck-Eidens ,  Bradley D. Swedlund

IPC分类号： C12N15/09 ,  C12N1/19 ,  C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12P9/00 ,  C12R1/19 ,  C12R1/865

CPC分类号： C12N9/1085

摘要： This invention provides a purified chrysanthemyl diphosphate synthase (CDS), a method for the purification of CDS from Chrysanthemum cinerariaefolium, and an amino acid sequence of the isolated CDS. Also provided is a cDNA encoding the CDS, a nucleotide sequence of the CDS gene, and a derived amino acid sequence of the encoded CDS protein. The CDS gene is useful in the enzymatic production of the natural stereospecific configuration of chrysanthemyl derivatives which are useful for the synthesis of pyrethrins, pyrethroids, derivatives thereof, as well as other classes of metabolites.

摘要翻译： 本发明提供了一种纯化的菊花二磷酸合成酶（CDS），一种从菊花中纯化CDS的方法和分离的CDS的氨基酸序列。 还提供了编码CDS的cDNA，CDS基因的核苷酸序列和编码的CDS蛋白的衍生氨基酸序列。 CDS基因可用于天然立体特异性构型的菊花衍生物的酶促生产，其可用于合成除虫菊酯，拟除虫菊酯，其衍生物以及其它类别的代谢物。