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公开(公告)号：US20100173408A1
公开(公告)日：2010-07-08
申请号：US12653484
申请日：2009-12-14
IPC分类号： C07K14/705 , C12N5/071 , C12N1/14 , C12N5/04
CPC分类号： C12N15/1055 , C07K14/71 , C07K14/715 , C07K2319/00 , C07K2319/02 , C07K2319/20 , C07K2319/70 , C07K2319/90 , C12N2799/027 , C12N2799/06
摘要： Described is a recombinant receptor comprising an extracellular ligand-binding domain and a cytoplasmic domain that comprises a heterologous bait polypeptide. The recombinant receptor is activated by binding of a ligand to the ligand-binding domain and by binding of a prey polypeptide to the heterologous bait peptide. The invention also discloses a method for detecting compound-compound binding using the recombinant receptor.
摘要翻译： 描述了包含细胞外配体结合结构域和包含异源诱饵多肽的细胞质结构域的重组受体。 通过将配体结合到配体结合结构域并通过将猎物多肽与异源诱饵肽的结合来激活重组受体。 本发明还公开了使用重组受体检测化合物 - 化合物结合的方法。
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公开(公告)号：US08003757B2
公开(公告)日：2011-08-23
申请号：US12653484
申请日：2009-12-14
IPC分类号： C07K19/00 , C12N5/10 , C12N1/15 , G01N33/566
CPC分类号： C12N15/1055 , C07K14/71 , C07K14/715 , C07K2319/00 , C07K2319/02 , C07K2319/20 , C07K2319/70 , C07K2319/90 , C12N2799/027 , C12N2799/06
摘要： Described is a recombinant receptor comprising an extracellular ligand-binding domain and a cytoplasmic domain that comprises a heterologous bait polypeptide. The recombinant receptor is activated by binding of a ligand to the ligand-binding domain and by binding of a prey polypeptide to the heterologous bait peptide. The invention also discloses a method for detecting compound-compound binding using the recombinant receptor.
摘要翻译： 描述了包含细胞外配体结合结构域和包含异源诱饵多肽的细胞质结构域的重组受体。 通过将配体结合到配体结合结构域并通过将猎物多肽与异源诱饵肽的结合来激活重组受体。 本发明还公开了使用重组受体检测化合物 - 化合物结合的方法。
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公开(公告)号：US07855270B2
公开(公告)日：2010-12-21
申请号：US10303157
申请日：2002-11-22
IPC分类号： C07K19/00 , C12N5/10 , C12N1/15 , G01N33/566
CPC分类号： C12N15/1055 , C07K14/71 , C07K14/715 , C07K2319/00 , C07K2319/02 , C07K2319/20 , C07K2319/70 , C07K2319/90 , C12N2799/027 , C12N2799/06
摘要： The present invention discloses a recombinant receptor comprising an extracellular ligand-binding domain and a cytoplasmic domain that comprises a heterologous bait polypeptide. The recombinant receptor is activated by binding of a ligand to the ligand binding domain and by binding of a prey polypeptide to the heterologous bait peptide. The present invention also discloses a method for detecting compound-compound binding using the recombinant receptor.
摘要翻译： 本发明公开了包含细胞外配体结合结构域和包含异源诱饵多肽的细胞质结构域的重组受体。 重组受体通过将配体结合到配体结合结构域并通过将猎物多肽与异源诱饵肽结合来活化。 本发明还公开了使用重组受体检测化合物 - 化合物结合的方法。
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公开(公告)号：US07291458B2
公开(公告)日：2007-11-06
申请号：US10235264
申请日：2002-09-04
IPC分类号： C12Q1/68
CPC分类号： C07K14/5759 , A61K38/2264 , A61K2300/00
摘要： Methods of activating a signaling cascade comprising, introducing leptin and/or a cytokine to a receptor complex comprising gp 130, optionally in combination with a compound acting on adenylate cyclase or acting on one or more downstream targets of adenylate cyclase, thereby inducing genes in neuro-endocrine cells or cells of neuro-endocrine origin. Two distinct gene-sets are induced, immediate early response genes (STAT-3, SOCS-3, Metallothionein-II, the serine/threonine kinase Fnk and the rat homologue of MRF-1), and late induced target genes (Pancreatitis Associated Protein I, Squalene Epoxidase, Uridinediphosphate Glucuronyl Transferase and Annexin VIII). Strong co-stimulation with the adenylate cyclase activator forskolin was shown with respect to late induced target genes. Transcripts encoding Leptin Induced Protein I (LIP-I) and Leptin Induced Protein II (LIP-II) were identified; however, no forskolin co-stimulatory effect was observed. It is also demonstrated that leptin modulates in vivo expression of MT-II, Fnk and Pancreatitis Associated Protein I genes.
摘要翻译： 激活信号级联的方法包括将瘦素和/或细胞因子引入到包含gp 130的受体复合物中，任选地与作用于腺苷酸环化酶的化合物组合或作用于腺苷酸环化酶的一个或多个下游靶标，从而诱导神经中的基因 内分泌细胞或神经内分泌源的细胞。 诱导两个不同的基因组，立即早期应答基因（STAT-3，SOCS-3，金属硫蛋白II，丝氨酸/苏氨酸激酶Fnk和MRF-1的大鼠同源物）和晚期诱导的靶基因（胰腺炎相关蛋白 I，角鲨烯环氧酶，二叉二磷酸葡糖醛酸转移酶和膜联蛋白VIII）。 与晚期诱导的靶基因相比，显示出与腺苷酸环化酶激活剂毛喉素的强共刺激。 鉴定了编码瘦素诱导蛋白I（LIP-I）和瘦蛋白诱导蛋白II（LIP-II）的转录物; 然而，没有观察到毛喉素共刺激作用。 还表明瘦素调节MT-II，Fnk和胰腺炎相关蛋白I基因的体内表达。
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