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    Reversed mammalian protein-protein interaction trap
    4.
    发明授权
    Reversed mammalian protein-protein interaction trap 有权
    反转的哺乳动物蛋白质 - 蛋白质相互作用陷阱

    公开(公告)号：US08048986B2

    公开(公告)日：2011-11-01

    申请号：US12653513

    申请日：2009-12-14

    申请人： Sven Eyckerman ,  Jan Tavernier ,  Joel S. Vandekerckhove

    发明人： Sven Eyckerman ,  Jan Tavernier ,  Joel S. Vandekerckhove

    IPC分类号： C07K14/715 ,  C07K19/00 ,  G01N33/567 ,  C07H21/04

    CPC分类号： C07K14/705 ,  C07K14/4703 ,  C07K14/4718 ,  C07K2319/00 ,  C12N15/1055

    摘要： The invention relates to a recombinant receptor, comprising a ligand-binding domain and a signaling domain that comprises a heterologous bait polypeptide, which receptor is inactivated by binding of a prey polypeptide to the heterologous bait peptide, either in presence or absence of a ligand binding to the ligand-binding domain. The receptor is activated by addition of a compound that disrupts the bait-prey interaction. The invention also relates to a method of screening compounds that disrupt compound-compound binding using the recombinant receptor.

    摘要翻译： 本发明涉及重组受体，其包含配体结合结构域和包含异源诱饵多肽的信号结构域，所述诱饵多肽在存在或不存在配体结合的情况下通过将猎物多肽与异源诱饵肽结合而失活 到配体结合结构域。 通过加入扰乱诱饵 - 捕食相互作用的化合物来激活受体。 本发明还涉及一种筛选使用重组受体破坏化合物 - 化合物结合的化合物的方法。

    Agents and procedures for the transfer of proteins and/or nucleic acids onto a supported receptor surface
    7.
    发明授权
    Agents and procedures for the transfer of proteins and/or nucleic acids onto a supported receptor surface 失效
    将蛋白质和/或核酸转移到受支持的受体表面上的试剂和程序

    公开(公告)号：US4839231A

    公开(公告)日：1989-06-13

    申请号：US92242

    申请日：1987-09-02

    申请人： Joel S. Vandekerckhove

    发明人： Joel S. Vandekerckhove

    IPC分类号： G01N27/447 ,  G01N33/545 ,  G01N33/552 ,  G01N33/558

    CPC分类号： G01N33/552 ,  G01N27/44717 ,  G01N33/545 ,  G01N33/558 ,  Y10T428/249956 ,  Y10T428/249962 ,  Y10T428/261 ,  Y10T428/31645 ,  Y10T428/31909

    摘要： The invention relates to a procedure for the transfer and immobilization of proteins onto a free surface formed on a chemically inert support, preferably porous glass, from a medium likely to contain them which is brought into contact with this surface. The said surface is composed of a film of a vinylpyridine polymer in which the nitrogen atom of the pyridyl moiety is substituted by an alkyl group, in particular by a methyl group; the proteins and/or the nucleic acids are brought into contact with the said surface in solution in the presence of a detergent bearing a negative charge, in particular SDS. The invention also relates more particularly to the supports coated with the said films, in particular as a monomolecular layer.

    摘要翻译： 本发明涉及将蛋白质从形成于化学惰性的载体（优选多孔玻璃）上的游离表面上转移和固定的方法，该方法可能包含与该表面接触的介质。 所述表面由乙烯基吡啶聚合物的膜组成，其中吡啶基部分的氮原子被烷基，特别是被甲基取代; 在携带负电荷的洗涤剂，特别是SDS的存在下，蛋白质和/或核酸与溶液中的所述表面接触。 本发明还特别涉及涂覆有所述膜的载体，特别是单分子层。