公开(公告)号：US20140057286A1

公开(公告)日：2014-02-27

申请号：US14051729

申请日：2013-10-11

申请人： Sysmex Corporation ,  National Center for Global Health and Medicine ,  Nagoya City University ,  National Institute of Advanced Industrial Science and Technology

发明人： Hisashi Narimatsu ,  Yuzuru Ikehara ,  Atsushi Kuno ,  Maki Sogabe ,  Yasuhito Tanaka ,  Masashi Mizokami ,  Kiyoaki Ito ,  Shunsuke Matsubara ,  Chikayuki Tsuruno ,  Youichi Takahama ,  Takashi Kagawa ,  Shinya Nagai

IPC分类号： G01N33/68

CPC分类号： G01N33/68 ,  C07K14/4726 ,  C07K14/473 ,  G01N33/5308 ,  G01N33/57438 ,  G01N33/6893 ,  G01N2333/4724 ,  G01N2333/4728 ,  G01N2400/00 ,  G01N2400/02 ,  G01N2800/085 ,  Y10T436/143333

摘要： An object of the present invention is to provide a method for measuring a glycan-marker glycoprotein, by which liver disease can be detected with higher accuracy than is possible with conventional methods. Also, an object of the present invention is to provide a method for examining liver disease, by which liver disease can be detected with higher accuracy than is possible with conventional methods. Also, an object of the present invention is to provide a reagent for quantitative determination of a glycoprotein, which is used for the above measurement methods. Furthermore, an object of the present invention is to provide a glycan-marker glycoprotein as an index for clinical conditions of liver disease, which is capable of identifying the clinical conditions of liver disease depending on the progress of liver disease. The method for measuring a glycoprotein is characterized in that: the glycoprotein is at least one glycoprotein selected from alpha-1-acid glycoprotein (AGP) and Mac-2-binding protein (M2BP) contained in a sample collected from a subject; when the glycoprotein is AGP, AGP binding to a first lectin selected from AOL and MAL is measured; and when the glycoprotein is M2BP, M2BP binding to a second lectin selected from WFA, BPL, AAL, RCA120, and TJAII is measured.

摘要翻译： 本发明的目的是提供一种测定聚糖标记糖蛋白的方法，通过该方法可以以比常规方法更高的精度检测肝脏疾病。 另外，本发明的目的在于提供一种检查肝脏疾病的方法，通过该方法能够以比常规方法更高的精度检测肝脏疾病。 另外，本发明的目的在于提供一种用于上述测定方法的糖蛋白定量试剂。 此外，本发明的目的是提供聚糖标记糖蛋白作为肝病临床病症的指标，其能够根据肝病的进展来鉴定肝脏疾病的临床状况。 测定糖蛋白的方法的特征在于：糖蛋白是从受试者收集的样品中含有的至少一种糖蛋白，其选自α-1-酸糖蛋白（AGP）和Mac-2结合蛋白（M2BP） 当糖蛋白为AGP时，测量与选自AOL和MAL的第一凝集素结合的AGP; 并且当糖蛋白为M2BP时，测量与选自WFA，BPL，AAL，RCA120和TJAII的第二凝集素结合的M2BP。

公开(公告)号：US20180106814A1

公开(公告)日：2018-04-19

申请号：US15809160

申请日：2017-11-10

申请人： SYSMEX CORPORATION ,  NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY ,  NAGOYA CITY UNIVERSITY ,  NATIONAL CENTER FOR GLOBAL HEALTH AND MEDICINE

发明人： Hisashi Narimatsu ,  Yuzuru Ikehara ,  Atsushi Kuno ,  Maki Sogabe ,  Yasuhito Tanaka ,  Masashi Mizokami ,  Kiyoaki Ito ,  Shunsuke Matsubara ,  Chikayuki Tsuruno ,  Youichi Takahama ,  Takashi Kagawa ,  Shinya Nagai

IPC分类号： G01N33/68 ,  G01N33/574 ,  C07K14/47

CPC分类号： G01N33/68 ,  C07K14/4726 ,  C07K14/473 ,  G01N33/5308 ,  G01N33/57438 ,  G01N33/6893 ,  G01N2333/4724 ,  G01N2333/4728 ,  G01N2400/00 ,  G01N2400/02 ,  G01N2800/085 ,  Y10T436/143333

摘要： An object of the present invention is to provide a method for measuring a glycan-marker glycoprotein, by which liver disease can be detected with higher accuracy than is possible with conventional methods. Also, an object of the present invention is to provide a method for examining liver disease, by which liver disease can be detected with higher accuracy than is possible with conventional methods. Also, an object of the present invention is to provide a reagent for quantitative determination of a glycoprotein, which is used for the above measurement methods. Furthermore, an object of the present invention is to provide a glycan-marker glycoprotein as an index for clinical conditions of liver disease, which is capable of identifying the clinical conditions of liver disease depending on the progress of liver disease. The method for measuring a glycoprotein is characterized in that: the glycoprotein is at least one glycoprotein selected from alpha-1-acid glycoprotein (AGP) and Mac-2-binding protein (M2BP) contained in a sample collected from a subject; when the glycoprotein is AGP, AGP binding to a first lectin selected from AOL and MAL is measured; and when the glycoprotein is M2BP, M2BP binding to a second lectin selected from WFA, BPL, AAL, RCA120, and TJAII is measured.

公开(公告)号：US20160304976A1

公开(公告)日：2016-10-20

申请号：US14914791

申请日：2014-08-28

申请人： THE UNIVERSITY OF TOKYO ,  NATIONAL CENTER FOR GLOBAL HEALTH AND MEDICINE

发明人： Katsushi Tokunaga ,  Hiromi Sawai ,  Masashi Mizokami ,  Nao Nishida

IPC分类号： C12Q1/70

CPC分类号： C12Q1/706 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/172

摘要： An object of the present invention is to provide a method for detecting predisposition for chronicity of hepatitis B and/or the pathological progress, including an allele associated with chronicity of hepatitis B and/or the pathological progress. The above-described object is achieved by providing a method that includes a process of identifying alleles which are susceptible and resistant to chronicity of hepatitis B and/or the pathological progress in which a difference between a chronic hepatitis B patient group and a healthy control group is accurately reflected and comparing the alleles with base sequences or amino acid sequences corresponding to the alleles in a specimen; a process of analyzing whether bases of sites corresponding to the alleles in the base sequences of the specimen match bases of the alleles; and a process of specifying whether the hepatitis B of the specimen becomes chronic and/or the pathology is progressed, a method of performing inspection using the method, a reagent used for the method, and a test kit including the reagent.

摘要翻译： 本发明的目的是提供一种用于检测乙型肝炎长期易感性和/或病理进展的方法，包括与乙型肝炎的慢性相关的等位基因和/或病理进展。 上述目的通过提供一种方法来实现，该方法包括鉴定易感和耐乙型肝炎慢性的等位基因的过程和/或慢性乙型肝炎患者组与健康对照组之间的差异的病理进展 准确地反映并比较样品中与等位基因相对应的碱基序列或氨基酸序列等位基因; 分析与样本的碱基序列中的等位基因相对应的位点的碱基是否与等位基因的碱基相匹配的过程; 以及确定样本的乙型肝炎是否变慢和/或病变进行的方法，使用该方法进行检查的方法，用于该方法的试剂以及包含该试剂的检测试剂盒的处理。

公开(公告)号：US11571473B2

公开(公告)日：2023-02-07

申请号：US16972367

申请日：2019-06-06

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY ,  UNIVERSITY OF TOYAMA

发明人： Hisashi Narimatsu ,  Kiyohiko Angata ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Sato ,  Yasunori Chiba ,  Akira Togayachi ,  Hiroki Shimizu ,  Maki Sogabe ,  Takanori Wagatsuma ,  Masashi Mizokami ,  Masaaki Korenaga ,  Kazuto Tajiri ,  Tatsuhiko Ozawa

IPC分类号： A61K39/29 ,  C07K14/005 ,  A61P31/20 ,  C07K16/08 ,  G01N33/576 ,  A61K39/00

摘要： An examination system that recognizes a glycosylated antigen in Dane particles of hepatitis B virus (HBV) and a neutralizing antibody that recognizes the glycosylated antigen and that exhibits an infection-inhibiting activity. It was elucidated that Dane particles are associated with specific glycan structures, and this enabled the construction of a new detection system for infectious, i.e., nucleic acid-containing, hepatitis B virus particles and the provision of a neutralizing antibody that recognizes a glycosylated antigen and that exhibits an infection-inhibiting activity.