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    Tm mapping method
    2.
    发明授权
    Tm mapping method 有权

    公开(公告)号：US11732311B2

    公开(公告)日：2023-08-22

    申请号：US16906115

    申请日：2020-06-19

    申请人： National University Corporation University of Toyama

    发明人： Hideki Niimi ,  Shinya Otsuki ,  Isao Kitajima

    IPC分类号： C12Q1/689 ,  G16B30/00

    CPC分类号： C12Q1/689 ,  G16B30/00

    摘要： The improved Tm mapping method using imperfect-match linear long quenching probes can accurately distinguish among and identify microorganisms at least at the genus level and often at the species level even in a real-time PCR instrument having measurement errors of Tm values between PCR tubes within ±0.5° C. Therefore, the Tm mapping method can be performed in almost all real-time PCR instruments and can identify unspecified infection-causing pathogenic microorganisms in about 4 hours after sample collection.

    BACTERIUM NUMBER MEASURING METHOD AND BACTERIUM NUMBER MEASURING SYSTEM
    4.
    发明申请
    BACTERIUM NUMBER MEASURING METHOD AND BACTERIUM NUMBER MEASURING SYSTEM 有权

    公开(公告)号：US20210317503A1

    公开(公告)日：2021-10-14

    申请号：US17224957

    申请日：2021-04-07

    申请人： HITACHI, LTD. ,  University of Toyama

    发明人： Shunsuke KAWABE ,  Yuichi UCHIHO ,  Hideyuki NODA ,  Hideki NIIMI ,  Atsushi MATSUI

    IPC分类号： C12Q1/18 ,  C12M1/32 ,  C12M1/36 ,  C12M1/34

    摘要： Provided is a bacterium number measuring system comprising a database storing known bacterial growth patterns in advance and an analyzing part. The analyzing part has first cultures containing a bacterial liquid that contains a measurement target bacteria and second cultures being different from the first cultures and containing the bacterial liquid and an antimicrobial drug. The analyzing part performs bacterium number measurement which measures the number of bacteria in the first cultures on a culturing part where culturing has started in the first and second cultures. The analyzing part compares the bacterium number measurement result with the growth curves stored in the database and thereby determines a timing to perform MIC measurement which measures the number of bacteria in the second cultures to determine a minimum inhibitory concentration of the measurement target bacterium. The analyzing part performs the MIC measurement at the thus-determined timing.

    Method for producing antigen specific monoclonal antibody
    8.
    发明授权
    Method for producing antigen specific monoclonal antibody 有权

    公开(公告)号：US10416165B2

    公开(公告)日：2019-09-17

    申请号：US15751792

    申请日：2016-08-10

    申请人： National University Corporation University of Toyama

    发明人： Nobuyuki Kurosawa ,  Masaharu Isobe

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C07K16/00 ,  A61K39/00 ,  C07K14/00 ,  G01N33/577 ,  C07K16/18 ,  C12N1/02 ,  C12N15/09 ,  G01N33/53 ,  C07K16/40 ,  C12N5/12 ,  C12N9/50 ,  C12N11/14

    摘要： A method for separating cells capable of producing target antigen-specific monoclonal antibodies (TASMAs) wherein a cell group including antibody-producing cells is immobilized using a reversible crosslinking agent having cell membrane-permeating properties. The immobilized cell group is subjected to cell membrane dissolution using a surface active agent; and the cell group is reacted with a labeling target antigen. In the stained cell group a that has reacted with the labeling target antigen is separated. A method to produce TASMAs by separating mRNA from the cell separated using the method; preparing cDNA and preparing antigen-specific monoclonal antibodies or fragments thereof from the prepared cDNA. Also provided are a method whereby at least one cell capable of producing TASMAs is separated and a method whereby said antibodies can be produced by using the separated cell. Threonine 18 phosphorylated p53 (pT18-p53) and threonine 68 phosphorylated CHK2 (pT68-CHK2) specific monoclonal antibodies are also disclosed.