利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

13 条记录 (耗时 0.036 秒)

    DNA polymerase mutant suited to nucleic acid amplification from RNA
    4.
    发明授权
    DNA polymerase mutant suited to nucleic acid amplification from RNA 有权

    公开(公告)号：US12018294B2

    公开(公告)日：2024-06-25

    申请号：US17257986

    申请日：2019-07-03

    申请人： TAKARA BIO INC.

    发明人： Takashi Uemori ,  Hiroyuki Matsumoto ,  Kensuke Saito ,  Miwa Akitomo

    IPC分类号： C12N9/12

    CPC分类号： C12N9/1252 ,  C12Y207/07007

    摘要： Provided are: a DNA polymerase mutant having reverse transcriptase activity, the DNA polymerase mutant including a sequence consisting of twelve specific amino acids A1-A12, wherein the DNA polymerase mutant having reverse transcriptase activity is characterized in that the A3 and/or A10 amino acid is substituted by a basic amino acid residue different from that prior to the introduction of mutation; a kit and a composition including the DNA polymerase; a method for producing the DNA polymerase; and a method for modifying an existing DNA polymerase having reverse transcriptase activity.

    Heat-resistant reverse transcriptase mutant
    5.
    发明授权
    Heat-resistant reverse transcriptase mutant 有权

    公开(公告)号：US11220677B2

    公开(公告)日：2022-01-11

    申请号：US16466352

    申请日：2017-12-13

    申请人： TAKARA BIO INC.

    发明人： Kazuhiko Ishikawa ,  Takashi Uemori ,  Nariaki Takatsu

    IPC分类号： C12N9/12 ,  C12N15/52 ,  C12Q1/686 ,  C12N15/09

    摘要： Provided are: a reverse transcriptase mutant including an amino acid mutation at a position corresponding to position 55 of the amino acid sequence of wild-type reverse transcriptase derived from the Moloney murine leukemia virus, wherein the reverse transcriptase mutant is characterized in that the amino acid mutation is a substitution from threonine to another amino acid, and the other amino acid is selected from the group consisting of amino acids having a nonpolar aliphatic side chain and amino acids having a polar acidic functional group side chain; a nucleic acid that encodes the mutant; a method for producing the mutant and the nucleic acid that encodes the mutant; a method for synthesizing cDNA in which the mutant is used; and a composition and kit including the mutant.

    METHOD FOR IMPROVING NUCLEIC ACID SYNTHESIS REACTION
    8.
    发明申请
    METHOD FOR IMPROVING NUCLEIC ACID SYNTHESIS REACTION 有权
    改善核酸合成反应的方法

    公开(公告)号：US20140363849A1

    公开(公告)日：2014-12-11

    申请号：US14372558

    申请日：2013-01-24

    申请人： TAKARA BIO INC.

    发明人： Kiyoyuki Matsumura ,  Takashi Uemori ,  Hiroyuki Mukai

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6848 ,  C12N9/1252 ,  C12N9/1276 ,  C12P19/34 ,  C12Q1/6844 ,  C12Y207/07007 ,  C12Q2527/125

    摘要： Provided are the following: a method, for improving reactivity of a nucleic acid synthesis reaction, comprising a step for adding an ω-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ω-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ω-amino acid.

    摘要翻译： 提供如下：一种用于提高核酸合成反应的反应性的方法，包括向反应溶液中加入ω-氨基酸的步骤; 用于核酸合成反应的组合物，其包含DNA聚合酶，反应缓冲液，至少一种引物，至少一种脱氧核糖核苷酸三磷酸和ω-氨基酸; 和用于核酸合成反应的反应缓冲液，其包含ω-氨基酸。

    Oligonucleotide preservation method
    9.
    发明授权
    Oligonucleotide preservation method 有权

    公开(公告)号：US11572580B2

    公开(公告)日：2023-02-07

    申请号：US16616532

    申请日：2018-06-06

    申请人： TAKARA BIO INC.

    发明人： Miwa Akitomo ,  Takashi Uemori

    IPC分类号： C12Q1/6806 ,  C07K14/245

    摘要： The present invention pertains to: an oligonucleotide preservation method; and a kit comprising an oligonucleotide. The present invention provides a method for stably preserving an oligonucleotide-containing solution by adding a nucleic acid-binding protein to said oligonucleotide-containing solution in advance.