公开(公告)号：US10975415B2

公开(公告)日：2021-04-13

申请号：US15507796

申请日：2015-09-09

申请人： TAKARA BIO INC. ,  KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION ,  EDUCATIONAL CORPORATION KANSAI BUNRI SOUGOUGAKUEN

发明人： Takashi Uemori ,  Yoshizumi Ishino ,  Takehiro Sagara ,  Sonoko Ishino ,  Takeshi Yamagami ,  Tsuyoshi Shirai

IPC分类号： C12Q1/68 ,  C12N9/22 ,  C12N15/09

摘要： A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.

公开(公告)号：US11046939B2

公开(公告)日：2021-06-29

申请号：US15779247

申请日：2016-11-24

申请人： KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION ,  TAKARA BIO INC.

发明人： Yoshizumi Ishino ,  Sonoko Ishino ,  Takeshi Yamagami ,  Takashi Uemori ,  Nariaki Takatsu

IPC分类号： C12N9/12 ,  C12N1/20 ,  C12N15/52 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12P19/34

摘要： The present invention relates to a Thermus aquaticus (Taq) polymerase having a strand displacement activity in which an amino acid residue in a template DNA binding site of the DNA polymerase is substituted with an amino acid to increase a total charge in the site, a nucleic acid encoding the polymerase, a vector containing the nucleic acid, a transformant containing the vector containing the nucleic acid or the nucleic acid, a method for producing the polymerase, a method for amplifying nucleic acids utilizing the polymerase, and a kit containing the polymerase. According to the present invention, a DNA polymerase having a high thermostability, capable of efficiently replicating a long-strand of a template DNA, and having a strong strand displacement activity is provided.

公开(公告)号：US10533162B2

公开(公告)日：2020-01-14

申请号：US15855882

申请日：2017-12-27

申请人： KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION

发明人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa

IPC分类号： C12N9/12 ,  C12N1/00 ,  C12N1/20 ,  C12N15/63 ,  C12P19/34

摘要： Provided are various novel DNA polymerases.Provided are: a DNA polymerase comprising: an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 12, which has a substitution of arginine at position 651 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid; and a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 14, which has a substitution of proline at position 653 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid.

公开(公告)号：US09840700B2

公开(公告)日：2017-12-12

申请号：US14890009

申请日：2014-05-09

申请人： Kyushu University, National University Corporation

发明人： Yoshizumi Ishino ,  Sonoko Ishino ,  Miyako Shiraishi

IPC分类号： C12N9/22 ,  C12N15/00 ,  C12N15/10

CPC分类号： C12N9/22 ,  C12N15/102 ,  C12Y301/00

摘要： There is provided an enzyme which has an activity of cleaving a phosphodiester bond of deoxyribonucleotide having a damaged base and deoxyribonucleotide adjacent to the 5′ side of the deoxyribonucleotide in DNA strands which contain the damaged base as a reagent or the like for manipulating a gene, and further provided a method of removing a damaged base from DNA strands using the enzyme.