公开(公告)号：US11981934B2

公开(公告)日：2024-05-14

申请号：US16958974

申请日：2018-12-27

Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED

Inventor： Christian Fiedler ,  Meinhard Hasslacher ,  Jadranka Koehn

IPC: C07K14/435 ,  A61K38/00 ,  B01D15/16 ,  B01D15/36 ,  B01D15/38 ,  B01D15/42 ,  C07K14/705 ,  C07K16/28 ,  C12N7/00 ,  G01N33/569

CPC classification number: C12N7/00 ,  B01D15/166 ,  B01D15/363 ,  B01D15/3809 ,  B01D15/426 ,  G01N33/56983 ,  C12N2750/14151 ,  G01N2333/075

Abstract: Provided herein are methods of producing an adeno-associated virus (AAV) product and methods of purifying adeno-associated virus. AAV is loaded onto an affinity resin, wash steps are undertaken, and AAV is eluted from the affinity resin. Various buffers are disclosed for use in the wash steps and elution.