公开(公告)号：US20230066806A1

公开(公告)日：2023-03-02

申请号：US17439328

申请日：2020-03-13

Applicant: The Regents of the University of California

Inventor： Theodore Lee Roth ,  Po-Yi Jonathan Li ,  Alexander Marson ,  Jasper Nies ,  Cody Mowery ,  Eric Shifrut ,  Franziska Blaeschke ,  Ryan Apathy

IPC: C12N15/10 ,  C12N15/90 ,  A61K35/17 ,  A61P35/00 ,  A61P17/00

Abstract: Provided herein are methods and compositions for identifying a targeted genomic insertion in a cell. Also provided are heterologous polypeptides that are co-expressed under the control of enodogenous loci and methods of using same.

公开(公告)号：US20230310502A1

公开(公告)日：2023-10-05

申请号：US18160825

申请日：2023-01-27

Applicant: The Regents of the University of California

Inventor： Theodore Lee Roth ,  Eric Shifrut ,  Alexander Marson ,  Cristina Puig Saus ,  Antoni Ribas

IPC: A61K35/17 ,  C07K14/725 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85 ,  C12N15/90

CPC classification number: A61K35/17 ,  C07K14/7051 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85 ,  C12N15/907 ,  C12N2310/20

Abstract: Provided herein are methods and compositions for editing the genome of a human T cell. In some embodiments, a heterologous T cell receptor (TCR)-β chain and a heterologous TCR-α chain are inserted into exon 1 of a TCR subunit constant gene in the genome of the T cell.

公开(公告)号：US11331346B2

公开(公告)日：2022-05-17

申请号：US17390673

申请日：2021-07-30

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Theodore Lee Roth ,  Eric Shifrut ,  Alexander Marson ,  Cristina Puig Saus ,  Antoni Ribas

IPC: A61K35/17 ,  C07K14/725 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85 ,  C12N15/90

Abstract: Provided herein are methods and compositions for editing the genome of a human T cell. In some embodiments, a heterologous T cell receptor (TCR)-β chain and a heterologous TCR-α chain are inserted into exon 1 of a TCR subunit constant gene in the genome of the T cell.

公开(公告)号：US20210340496A1

公开(公告)日：2021-11-04

申请号：US17284396

申请日：2019-10-10

Applicant: The Regents of the University of California

Inventor： Alexander Marson ,  Jessica T. Cortez ,  Jeffrey A. Bluestone ,  Eric Shifrut ,  Frederic Van Gool

IPC: C12N5/0783 ,  C12N15/90 ,  A61K35/17 ,  C12N15/11 ,  C12N9/22

Abstract: Provided herein are compositions and methods for modifying regulatory T cells. The inventors have identified nuclear factors that influence expression of Foxp3, a key transcriptional regulator of Treg cells. Treg cells can be modified by inhibiting and/or overexpressing one or more of these nuclear factors to produce stabilized Treg cells or destabilized Treg cells.

公开(公告)号：US20250041338A1

公开(公告)日：2025-02-06

申请号：US18710157

申请日：2022-11-17

Applicant: The Regents of the University of California ,  The J. David Gladstone Institutes, a testamentary trust established under the will of J. David

Inventor： Alexander Marson ,  Eric Shifrut ,  Julia Carnevale ,  Alan Ashworth

IPC: A61K35/17 ,  A61K39/00 ,  A61P35/00 ,  C12N5/0783 ,  C12N15/90

Abstract: Provided herein are genetically modified T cells that exhibit increased proliferation compared to wild-type T cells when stimulated, methods of generating such T cells, and methods of using the T cells for the treatment of a disease such as cancer.

公开(公告)号：US11590171B2

公开(公告)日：2023-02-28

申请号：US17725478

申请日：2022-04-20

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Theodore Lee Roth ,  Eric Shifrut ,  Alexander Marson ,  Cristina Puig Saus ,  Antoni Ribas

IPC: A61K35/17 ,  C07K14/725 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85 ,  C12N15/90

Abstract: Provided herein are methods and compositions for editing the genome of a human T cell. In some embodiments, a heterologous T cell receptor (TCR)-β chain and a heterologous TCR-α chain are inserted into exon 1 of a TCR subunit constant gene in the genome of the T cell.

公开(公告)号：US11033584B2

公开(公告)日：2021-06-15

申请号：US16568116

申请日：2019-09-11

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Theodore Lee Roth ,  Eric Shifrut ,  Alexander Marson ,  Cristina Puig Saus ,  Antoni Ribas

IPC: A61K35/17 ,  C07K14/725 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85 ,  C12N15/90

Abstract: Provided herein are methods and compositions for editing the genome of a human T cell. In some embodiments, a heterologous T cell receptor (TCR)-β chain and a heterologous TCR-α chain are inserted into exon 1 of a TCR subunit constant gene in the genome of the T cell.