CONJUGATED POLYMERS FOR USE IN HOMOGENEOUS AND SOLID STATE ASSAYS
    6.
    发明申请
    CONJUGATED POLYMERS FOR USE IN HOMOGENEOUS AND SOLID STATE ASSAYS 有权
    共聚聚合物用于均质和固体状态测定

    公开(公告)号:US20130295684A1

    公开(公告)日:2013-11-07

    申请号:US13726520

    申请日:2012-12-24

    Abstract: Disclosed are multichromophores, and methods, articles and compositions employing them. Disclosed are methods, articles and compositions for the detection and analysis of biomolecules in a sample. Provided assays include those determining the presence of a target biomolecule in a sample or its relative amount, or the assays may be quantitative or semi-quantitative. The methods can be performed on a substrate or in an array format on a substrate. Disclosed are detection assays employing sensor biomolecules that do not comprise a fluorophore that can exchange energy with the cationic multichromophore. Disclosed are biological assays in which energy is transferred between one or more of the multichromophore, a label on the target biomolecule, a label on the sensor biomolecule, and/or a fluorescent dye specific for a polynucleotide, in all permutations.

    Abstract translation: 公开了多重色谱和其使用它们的方法,制品和组合物。 公开了用于检测和分析样品中生物分子的方法,制品和组合物。 提供的测定包括确定样品中靶生物分子的存在或其相对量的那些,或者测定可以是定量或半定量的。 该方法可以在衬底上以阵列形式在衬底上进行。 公开了使用不包含可与阳离子多重色素交换能量的荧光团的传感器生物分子的检测测定。 公开了在所有排列中能量在一个或多个多色素,目标生物分子上的标记,传感器生物分子上的标记和/或多核苷酸特异的荧光染料之间转移能量的生物测定。

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