公开(公告)号：US20170266440A1

公开(公告)日：2017-09-21

申请号：US15470542

申请日：2017-03-27

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Wentai Liu ,  James C.Y. Dunn ,  Benjamin M. Wu ,  Yi-Kai Lo ,  Chih-Wei Chang ,  Justin P. Wagner

IPC分类号： A61N1/36 ,  A61N1/08

CPC分类号： A61N1/36007 ,  A61B5/4233 ,  A61B5/4238 ,  A61B5/4255 ,  A61F5/0026 ,  A61N1/0509 ,  A61N1/0517 ,  A61N1/08

摘要： A gastrointestinal stimulation apparatus and methods with an electronic controller and a flexible and stretchable electrode array with a central branch and orthogonal bilateral branches that wrap around a section of the gastrointestinal tract and can accommodate repetitive contraction and relaxation movements of the tract. Array branches have a flexible spring structure, stimulation electrodes, recording electrodes, sensors controlled by a controller and adhesion nodes that fix the branches to the tissue. The electrode array can sense the normal peristalsis from upstream tissue and produce a stimulus signal to stimulate the incapable intestine section to generate stimulation-induced contractions. Electrodes on the incapable intestine section can be used for stimulation or recording, the recorded signal from the incapable intestine section can be sent back to the electronics to form a closed loop control system. An impedance measurement using current stimulation can be used to capture low frequency contraction signals.

公开(公告)号：US20160068805A1

公开(公告)日：2016-03-10

申请号：US14853564

申请日：2015-09-14

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  THE UNITED STATES GOVERNMENT REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS

发明人： Martin G. Martin ,  James C.Y. Dunn ,  Matthias Stelzner

IPC分类号： C12N5/071 ,  A61K35/38

CPC分类号： C12N5/068 ,  A61K35/38 ,  A61K48/00 ,  C12N5/0679 ,  C12N2501/11 ,  C12N2501/40 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2502/23

摘要： A cell culture media composition for support growth of human SI stem cells and epithelium without a feeder layer is presented. The media may also include growth factors including ENR and Y-27632 that support the survival of stem cell spheroid structures. The cell culture media compositions permit rapid growth of human small intestinal (SI) epithelium and stem cells, which leads to specific spheroid cell and enteroid morphology when grown in 3-D culture system.

摘要翻译： 提出了用于支持人SI干细胞和没有饲养层的上皮的生长的细胞培养基组合物。 培养基还可能包括生长因子，包括支持干细胞球体结构存活的ENR和Y-27632。 细胞培养基组合物允许人类小肠（SI）上皮细胞和干细胞的快速生长，当在3-D培养系统中生长时，其导致特定的球形细胞和肠形成形态。