公开(公告)号：US20170067030A1

公开(公告)日：2017-03-09

申请号：US15258584

申请日：2016-09-07

申请人： The Research Foundation for The State University of New York

发明人： Eckard Wimmer ,  Steve Skiena ,  Steffen Mueller ,  Bruce Futcher ,  Dimitris Papamichail ,  John Robert Coleman ,  Jeronimo Cello

IPC分类号： C12N7/00 ,  A61K39/13 ,  A61K39/145 ,  C07K14/005

CPC分类号： C12N7/00 ,  A61K39/13 ,  A61K39/145 ,  A61K2039/5254 ,  C07K14/005 ,  C12N2720/12361 ,  C12N2740/15061 ,  C12N2760/16061 ,  C12N2760/16121 ,  C12N2760/16134 ,  C12N2760/16162 ,  C12N2770/20061 ,  C12N2770/24161 ,  C12N2770/32061 ,  C12N2770/32621 ,  C12N2770/32634 ,  C12N2770/32661 ,  C12N2770/32662 ,  C12N2770/32671 ,  C12N2770/32721 ,  C12N2770/32761 ,  C12N2770/32762 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/466

摘要： This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.

摘要翻译： 本发明提供了一种减毒病毒，其包含经修饰的病毒基因组，其含有在基因组中多个位置工程改造的核苷酸取代，其中所述取代引入同义脱酮密码子进入基因组。 本发明的减毒病毒可用于疫苗组合物中以在受试者中诱导保护性免疫应答。 本发明还提供了合成即时减毒病毒的方法。 此外，本发明进一步提供了一种防止受试者患有病毒相关疾病的方法，包括向受试者施用预防有效剂量的包含即时减毒病毒的疫苗组合物。

公开(公告)号：US20140356962A1

公开(公告)日：2014-12-04

申请号：US14365854

申请日：2012-12-14

申请人： THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK

发明人： Eckard Wimmer ,  Jeronimo Cello ,  Ying Liu

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  C12N2770/32321 ,  C12N2770/32334 ,  C12N2770/32361 ,  C12N2770/32662

摘要： A novel and stable attenuated poliovirus is produced by engineering an indigenous replication element (cre), into the 5′ non-translated genomic region (with inactivation of the native ere element located in the coding region of 2C (mono-crePV), and replacing the nucleic acid sequence of all or part of the capsid coding region (PI) with a substitute PI coding region having reduced codon pair bias. The stably attenuated poliovirus is effective for vaccines and immunization.

摘要翻译： 通过将本地复制元件（cre）工程化到5'非翻译基因组区域（通过位于2C（单克隆抗体）编码区域中的天然元件的失活）来产生新的和稳定的减毒脊髓灰质炎病毒， 全部或部分衣壳编码区（PI）的核酸序列与具有降低的密码子对偏差的替代PI编码区。稳定减毒的脊髓灰质炎病毒对于疫苗和免疫是有效的。

公开(公告)号：US10023845B2

公开(公告)日：2018-07-17

申请号：US15258584

申请日：2016-09-07

申请人： The Research Foundation for The State University of New York

发明人： Eckard Wimmer ,  Steve Skiena ,  Steffen Mueller ,  Bruce Futcher ,  Dimitris Papamichail ,  John Robert Coleman ,  Jeronimo Cello

IPC分类号： C12N7/00 ,  A61K39/13 ,  A61K39/145 ,  C07K14/005 ,  A61K39/00

摘要： This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.