公开(公告)号：US20220228133A1

公开(公告)日：2022-07-21

申请号：US17613172

申请日：2020-05-22

Applicant: TOOLGEN INCORPORATED

Inventor： Sung Min BAE, ,  Young Hoon KIM ,  Jeong Joon LEE

IPC: C12N9/22 ,  C12N9/78 ,  C12N9/24 ,  C07K14/47 ,  C12N15/11 ,  C12N15/90

Abstract: The present application relates to: a single base substitution protein; a composition comprising same; and a use thereof.

公开(公告)号：US20220204955A1

公开(公告)日：2022-06-30

申请号：US17606502

申请日：2019-04-26

Applicant: TOOLGEN INCORPORATED

Inventor： Joon Sun LEE ,  Min Hee JUNG ,  Yu Ri CHOI ,  Jeong Joon LEE

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/88 ,  C12N15/85

Abstract: The present invention relates to an artificially engineered CRISPR/Cas9 system. More particularly, the present invention relates to an artificially engineered CRISPR enzyme having enhanced target specificity and a use of an artificially engineered CRISPR/Cas9 system including the same enzyme in genome and/or epigenome manipulation or modification, genome targeting, genome editing, and in vitro diagnosis, etc.

公开(公告)号：US20190177710A1

公开(公告)日：2019-06-13

申请号：US16310114

申请日：2017-06-14

Applicant: TOOLGEN INCORPORATED

Inventor： Jeong Joon LEE

IPC: C12N9/22 ,  C12Q1/44 ,  C12N15/11

Abstract: The present invention relates to a method for selecting a target specific nuclease having high specificity and high activity and, more particularly, to a method for screening and selecting a target specific nuclease system by using a multiple target system capable of simultaneously identifying off-target activity and on-target activity. Through the present invention, it is possible to select a target specific nuclease which has a decreased off-target effect and an increased on-target effect.