公开(公告)号：US20160002741A1

公开(公告)日：2016-01-07

申请号：US14857187

申请日：2015-09-17

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Shiro KITANO ,  Takeshi Yamada

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156 ,  G01N33/57484 ,  G01N2333/914 ,  G01N2800/52

Abstract: A method for predicting sensitivity to an EGFR inhibitor includes: (a) determining whether there is a KRAS gene-derived nucleic acid or a protein thereof in a blood sample which has been collected from a subject, and whether the KRAS gene-derived nucleic acid or the protein thereof in the blood sample is wild type or mutant; and (b) determining that there is a high possibility that a tumor of the subject is sensitive to an EGFR inhibitor when a wild type KRAS gene-derived nucleic acid or a protein thereof is detected and no mutant KRAS gene-derived nucleic acid or a protein thereof is detected in the blood sample in the process (a).

Abstract translation: 用于预测对EGFR抑制剂的敏感性的方法包括：（a）在从受试者收集的血液样品中确定是否存在KRAS基因衍生的核酸或其蛋白质，以及KRAS基因衍生的核酸 或其血液样品中的蛋白质是野生型或突变体; 和（b）当检测到野生型KRAS基因衍生的核酸或其蛋白质时，确定受试者的肿瘤对EGFR抑制剂敏感的可能性很高，并且没有突变体KRAS基因衍生的核酸或 在方法（a）中在血液样品中检测其蛋白质。

公开(公告)号：US11746382B2

公开(公告)日：2023-09-05

申请号：US16506489

申请日：2019-07-09

Applicant: TOPPAN PRINTING CO., LTD. ,  NIPPON MEDICAL SCHOOL FOUNDATION

Inventor： Shiro Kitano ,  Takeshi Yamada ,  Goro Takahashi ,  Takuma Iwai ,  Koki Takeda

IPC: C07H21/04 ,  C12Q1/68 ,  C12Q1/6886 ,  G16B40/10 ,  C12Q1/6827 ,  C12Q1/6851 ,  C12Q1/686

CPC classification number: C12Q1/6886 ,  C12Q1/686 ,  C12Q1/6827 ,  C12Q1/6851 ,  G16B40/10

Abstract: A method of detecting somatic mutations of tumor marker genes includes recovering a peripheral blood mononuclear cell layer from a blood sample collected from a subject and examining whether or not a nucleic acid derived from tumor marker genes having somatic mutations is detected from DNA included in the peripheral blood mononuclear cell layer.

公开(公告)号：US20180135138A1

公开(公告)日：2018-05-17

申请号：US15870190

申请日：2018-01-12

Applicant: TOPPAN PRINTING CO., LTD. ,  NIPPON MEDICAL SCHOOL FOUNDATION

Inventor： Shiro Kitano ,  Takeshi Yamada ,  Takuma Iwai

IPC: C12Q1/6886

CPC classification number: C12Q1/6886 ,  A61K31/282 ,  A61K31/513 ,  A61K31/519 ,  A61K31/7068 ,  A61K39/3955 ,  C07K16/22 ,  C07K16/2863 ,  C07K2317/24 ,  C07K2317/76 ,  C12Q2600/106 ,  C12Q2600/156 ,  G01N2800/52 ,  A61K2300/00

Abstract: A method for evaluating a health condition, includes: measuring a test amount of cell free DNA per unit amount of a body fluid sample collected from a subject after tumor resection; and comparing the measured test amount of cell free DNA with a reference value so as to evaluate a health condition of the subject. The reference value is one of a predetermined threshold value; an amount of cell free DNA per unit amount of a body fluid sample collected from the subject before tumor resection; an amount of cell free DNA per unit amount of a body fluid sample collected from the subject between 1 week and 3 months after tumor resection; and an amount of cell free DNA per unit amount of a body fluid sample collected from the subject by the time of collecting the body fluid sample for measuring the test amount of cell free DNA.

公开(公告)号：US20190330705A1

公开(公告)日：2019-10-31

申请号：US16506489

申请日：2019-07-09

Applicant: TOPPAN PRINTING CO., LTD. ,  NIPPON MEDICAL SCHOOL FOUNDATION

Inventor： Shiro KITANO ,  Takeshi Yamada ,  Goro Takahashi ,  Takuma Iwai ,  Koki Takeda

IPC: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6851 ,  G16B40/10

Abstract: A method of detecting somatic mutations of tumor marker genes includes recovering a peripheral blood mononuclear cell layer from a blood sample collected from a subject and examining whether or not a nucleic acid derived from tumor marker genes having somatic mutations is detected from DNA included in the peripheral blood mononuclear cell layer.