公开(公告)号：US20210115377A1

公开(公告)日：2021-04-22

申请号：US17050734

申请日：2019-05-07

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12M1/12 ,  D01F8/02

Abstract: The present invention relates to an extracellular-matrix-containing composition comprising: a fragmented extracellular matrix component; and an aqueous medium.

公开(公告)号：US20190049432A1

公开(公告)日：2019-02-14

申请号：US16164989

申请日：2018-10-19

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Shiro KITANO ,  Kei TSUKAMOTO ,  Shinji IRIE

IPC: G01N33/50

Abstract: A method of assessing an anti-cancer drug including culturing a cell structure including cancer cells and stromal cells in a presence of at least one anti-cancer drug, and assessing an anti-cancer effect of the at least one anti-cancer drug based on a number of viable cancer cells in the cell structure after the culturing.

公开(公告)号：US20200172874A1

公开(公告)日：2020-06-04

申请号：US16782579

申请日：2020-02-05

Applicant: TOPPAN PRINTING CO., LTD. ,  Japanese Foundation For Cancer Research

Inventor： Yuki TAKAHASHI ,  Shiro KITANO ,  Ryohei KATAYAMA ,  Satoshi NAGAYAMA

IPC: C12N5/09

Abstract: A primary culture method in which cells contained in a tissue collected from a living body are primary cultured in vitro, in which the cells in the tissue collected from the living body are seeded and cultured on a top surface of a cell structure containing cells constituting a stroma and composed of a single layer or two or more cell layers laminated in the thickness direction.

公开(公告)号：US20180230452A1

公开(公告)日：2018-08-16

申请号：US15948124

申请日：2018-04-09

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Hiroki NAGASE ,  Kei TSUKAMOTO ,  Shiro KITANO

IPC: C12N15/10 ,  C08G73/06 ,  C08G69/10 ,  G01N1/40

CPC classification number: C12N15/1006 ,  C07H1/06 ,  C07H21/00 ,  C08G69/10 ,  C08G73/0611 ,  C08G73/0616 ,  C12N15/09 ,  G01N1/405 ,  C12Q2525/191 ,  C12Q2537/159

Abstract: A method of separating a target double-stranded nucleic acid molecule from a sample including the target double-stranded nucleic acid molecule and a non-target double-stranded nucleic acid molecule, including (1) mixing the sample, a pyrrole-imidazole-containing polyamide (first PI polyamide) modified with a first linker molecule and capable of specifically binding to a sequence of the target double-stranded nucleic acid molecule, and a carrier a modified with a first ligand capable of specifically binding and/or adsorbing to the first linker molecule such that a mixed solution is produced, (2) forming a complex A by binding the carrier a to the first PI polyamide with which the target double-stranded nucleic acid molecule is bound in the mixed solution, and (3) separating the complex A from the mixed solution.

公开(公告)号：US20160002741A1

公开(公告)日：2016-01-07

申请号：US14857187

申请日：2015-09-17

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Shiro KITANO ,  Takeshi Yamada

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156 ,  G01N33/57484 ,  G01N2333/914 ,  G01N2800/52

Abstract: A method for predicting sensitivity to an EGFR inhibitor includes: (a) determining whether there is a KRAS gene-derived nucleic acid or a protein thereof in a blood sample which has been collected from a subject, and whether the KRAS gene-derived nucleic acid or the protein thereof in the blood sample is wild type or mutant; and (b) determining that there is a high possibility that a tumor of the subject is sensitive to an EGFR inhibitor when a wild type KRAS gene-derived nucleic acid or a protein thereof is detected and no mutant KRAS gene-derived nucleic acid or a protein thereof is detected in the blood sample in the process (a).

Abstract translation: 用于预测对EGFR抑制剂的敏感性的方法包括：（a）在从受试者收集的血液样品中确定是否存在KRAS基因衍生的核酸或其蛋白质，以及KRAS基因衍生的核酸 或其血液样品中的蛋白质是野生型或突变体; 和（b）当检测到野生型KRAS基因衍生的核酸或其蛋白质时，确定受试者的肿瘤对EGFR抑制剂敏感的可能性很高，并且没有突变体KRAS基因衍生的核酸或 在方法（a）中在血液样品中检测其蛋白质。

公开(公告)号：US20210063374A1

公开(公告)日：2021-03-04

申请号：US16644059

申请日：2018-09-03

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Shiro KITANO ,  Shinji IRIE

IPC: G01N33/15 ,  C12N5/077 ,  C07K14/78

Abstract: The present invention relates to an abnormal cardiac rhythm myocardial model composed of a three-dimensional tissue containing cells including the cardiomyocytes and collagen, wherein at least a portion of the cells adheres to the collagen.

公开(公告)号：US20190330705A1

公开(公告)日：2019-10-31

申请号：US16506489

申请日：2019-07-09

Applicant: TOPPAN PRINTING CO., LTD. ,  NIPPON MEDICAL SCHOOL FOUNDATION

Inventor： Shiro KITANO ,  Takeshi Yamada ,  Goro Takahashi ,  Takuma Iwai ,  Koki Takeda

IPC: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6851 ,  G16B40/10

Abstract: A method of detecting somatic mutations of tumor marker genes includes recovering a peripheral blood mononuclear cell layer from a blood sample collected from a subject and examining whether or not a nucleic acid derived from tumor marker genes having somatic mutations is detected from DNA included in the peripheral blood mononuclear cell layer.

公开(公告)号：US20230407266A1

公开(公告)日：2023-12-21

申请号：US18239938

申请日：2023-08-30

Applicant: TOPPAN PRINTING CO., LTD. ,  Japanese Foundation For Cancer Research

Inventor： Yuki TAKAHASHI ,  Shiro KITANO ,  Ryohei KATAYAMA ,  Satoshi NAGAYAMA

IPC: C12N5/09

CPC classification number: C12N5/0693 ,  C12N2502/11 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2509/10 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2533/90

Abstract: A primary culture method in which cells contained in a tissue collected from a living body are primary cultured in vitro, in which the cells in the tissue collected from the living body are seeded and cultured on a top surface of a cell structure containing cells constituting a stroma and composed of a single layer or two or more cell layers laminated in the thickness direction.

公开(公告)号：US20220133952A1

公开(公告)日：2022-05-05

申请号：US17502496

申请日：2021-10-15

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Shinji IRIE ,  Shiro KITANO

IPC: A61L27/24 ,  A61L27/44 ,  A61L27/54 ,  A61L31/04 ,  C12N5/00 ,  A61L27/38

Abstract: A method may comprises bringing cells suspended in an aqueous medium into contact with a plurality of fragmented collagen pieces and, after the cells brought into contact with the plurality of fragmented collagen pieces and the plurality of fragmented collagen pieces are concentrated, culturing the cells brought into contact with the fragmented collagen pieces, with the plurality of fragmented collagen pieces, to form a three-dimensional tissue.

公开(公告)号：US20210079375A1

公开(公告)日：2021-03-18

申请号：US16951456

申请日：2020-11-18

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Hiroki NAGASE ,  Kei TSUKAMOTO ,  Shiro KITANO

IPC: C12N15/10 ,  C07H1/06 ,  C07H21/00 ,  C08G69/10 ,  C08G73/06 ,  G01N1/40 ,  C12N15/09

Abstract: A method of separating a target double-stranded nucleic acid molecule from a sample including the target double-stranded nucleic acid molecule and a non-target double-stranded nucleic acid molecule, including (1) mixing the sample, a pyrrole-imidazole-containing polyamide (first PI polyamide) modified with a first linker molecule and capable of specifically binding to a sequence of the target double-stranded nucleic acid molecule, and a carrier a modified with a first ligand capable of specifically binding and/or adsorbing to the first linker molecule such that a mixed solution is produced, (2) forming a complex A by binding the carrier a to the first PI polyamide with which the target double-stranded nucleic acid molecule is bound in the mixed solution, and (3) separating the complex A from the mixed solution.