公开(公告)号：US11970549B2

公开(公告)日：2024-04-30

申请号：US17996601

申请日：2021-12-10

申请人： TOYOBO CO., LTD. ,  NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMA

发明人： Takafumi Yoshikane ,  Nobuyuki Kurosawa ,  Masaharu Isobe

IPC分类号： C07K16/40

CPC分类号： C07K16/40 ,  C07K2317/565

摘要： The invention provides an antibody that specifically binds to a 5′ to 3′ exonuclease domain of a DNA polymerase, or a fragment thereof. The antibody inhibits the 5′ to 3′ exonuclease activity of a DNA polymerase, or a fragment thereof.

公开(公告)号：US10416165B2

公开(公告)日：2019-09-17

申请号：US15751792

申请日：2016-08-10

申请人： National University Corporation University of Toyama

发明人： Nobuyuki Kurosawa ,  Masaharu Isobe

IPC分类号： C07H21/02 ,  C07H21/04 ,  C07K16/00 ,  A61K39/00 ,  C07K14/00 ,  G01N33/577 ,  C07K16/18 ,  C12N1/02 ,  C12N15/09 ,  G01N33/53 ,  C07K16/40 ,  C12N5/12 ,  C12N9/50 ,  C12N11/14

摘要： A method for separating cells capable of producing target antigen-specific monoclonal antibodies (TASMAs) wherein a cell group including antibody-producing cells is immobilized using a reversible crosslinking agent having cell membrane-permeating properties. The immobilized cell group is subjected to cell membrane dissolution using a surface active agent; and the cell group is reacted with a labeling target antigen. In the stained cell group a that has reacted with the labeling target antigen is separated. A method to produce TASMAs by separating mRNA from the cell separated using the method; preparing cDNA and preparing antigen-specific monoclonal antibodies or fragments thereof from the prepared cDNA. Also provided are a method whereby at least one cell capable of producing TASMAs is separated and a method whereby said antibodies can be produced by using the separated cell. Threonine 18 phosphorylated p53 (pT18-p53) and threonine 68 phosphorylated CHK2 (pT68-CHK2) specific monoclonal antibodies are also disclosed.

公开(公告)号：US20180292407A1

公开(公告)日：2018-10-11

申请号：US15751792

申请日：2016-08-10

申请人： National University Corporation University of Toyama

发明人： Nobuyuki Kurosawa ,  Masaharu Isobe

IPC分类号： G01N33/577 ,  C07K16/40

CPC分类号： G01N33/577 ,  C07K16/18 ,  C07K16/40 ,  C07K2317/565 ,  C12N1/02 ,  C12N5/12 ,  C12N9/50 ,  C12N11/14 ,  C12N15/09 ,  G01N33/53

摘要： A method for separating cells capable of producing target antigen-specific monoclonal antibodies (TASMAs) wherein a cell group including antibody-producing cells is immobilized using a reversible crosslinking agent having cell membrane-permeating properties. The immobilized cell group is subjected to cell membrane dissolution using a surface active agent; and the cell group is reacted with a labeling target antigen. In the stained cell group a that has reacted with the labeling target antigen is separated. A method to produce TASMAs by separating mRNA from the cell separated using the method; preparing cDNA and preparing antigen-specific monoclonal antibodies or fragments thereof from the prepared cDNA. Also provided are a method whereby at least one cell capable of producing TASMAs is separated and a method whereby said antibodies can be produced by using the separated cell. Threonine 18 phosphorylated p53 (pT18-p53) and threonine 68 phosphorylated CHK2 (pT68-CHK2) specific monoclonal antibodies are also disclosed.