公开(公告)号：US20200248164A1

公开(公告)日：2020-08-06

申请号：US16777128

申请日：2020-01-30

申请人： TOYOTA JIDOSHA KABUSHIKI KAISHA

发明人： Masayoshi MURAMATSU ,  Shusei OBATA

IPC分类号： C12N9/88 ,  C12N1/20 ,  C12P5/02

摘要： The present disclosure is intended to identify a substitution mutation that improves enzyme activity of a decarbonylase. An amino acid corresponding to an amino acid 90, 107, 163, or 171 in SEQ ID NO: 2 is to be substituted with an amino acid exhibiting a higher degree of hydrophobicity.

公开(公告)号：US20180201910A1

公开(公告)日：2018-07-19

申请号：US15867922

申请日：2018-01-11

申请人： TOYOTA JIDOSHA KABUSHIKI KAISHA

发明人： Masayoshi MURAMATSU ,  Shusei OBATA

IPC分类号： C12N9/02 ,  C12P5/02 ,  C12N9/88 ,  C07K14/405 ,  C12N15/70

CPC分类号： C12N9/0008 ,  C07K14/405 ,  C12N9/88 ,  C12N15/70 ,  C12P5/02 ,  C12Y102/0108

摘要： The capacity for alkane synthesis is to be significantly improved with the use of an acyl-ACP reductase gene and a decarbonylase gene derived from blue-green algae. A recombinant microorganism comprising, as foreign genes, an acyl-ACP reductase gene and a decarbonylase gene derived from blue-green algae comprises 4 types of ferredoxin genes and 3 types of ferredoxin reductase genes derived from blue-green algae in predetermined combinations.