公开(公告)号：US20240219380A1

公开(公告)日：2024-07-04

申请号：US18557446

申请日：2022-04-29

申请人： TRANSLATE BIO, INC.

发明人： Jonathan ABYSALH ,  Anusha DIAS ,  Jeffrey S. DUBINS ,  Jorel E. VARGAS

IPC分类号： G01N33/53

CPC分类号： G01N33/5308

摘要： The present invention provides, among other things, methods of measuring homopolymeric nucleotide lengths in a nucleic acid, including mRNA. In some aspects, provided herein is a method of measuring poly A tail length in mRNA comprising binding of mRNA with a minor-groove binding dye, followed by ribonuclease digestion and capillary electrophoresis.

公开(公告)号：US20230357751A1

公开(公告)日：2023-11-09

申请号：US18247607

申请日：2021-10-01

申请人： TRANSLATE BIO, INC.

发明人： Jonathan ABYSALH ,  Frank DEROSA ,  Cameron SMITH ,  Jorel E. VARGAS

IPC分类号： B04B11/08 ,  B01D33/46 ,  B01D61/14 ,  B01D61/18 ,  C12N15/10 ,  B04B3/00 ,  B04B7/18 ,  B04B15/06 ,  B01D39/08 ,  B01D63/16 ,  B01D33/11

CPC分类号： C12N15/1017 ,  B01D33/11 ,  B01D33/463 ,  B01D39/083 ,  B01D61/145 ,  B01D61/147 ,  B01D61/18 ,  B01D63/16 ,  B04B3/00 ,  B04B7/18 ,  B04B11/08 ,  B04B15/06 ,  B01D2239/1216 ,  B01D2315/02

摘要： The present invention relates, in part, to methods, systems and processes for large-scale purification of mRNA using a filtering centrifuge operating at lower gravitational forces. The invention also relates to compositions of purified mRNA and uses thereof.