公开(公告)号：US20080293148A1

公开(公告)日：2008-11-27

申请号：US11662914

申请日：2005-09-15

申请人： Tae Song Kim ,  Dae Sung Yoon ,  Kyo Seon Hwang ,  Jeong Hoon Lee

发明人： Tae Song Kim ,  Dae Sung Yoon ,  Kyo Seon Hwang ,  Jeong Hoon Lee

IPC分类号： G01N33/00 ,  B01J19/00 ,  G01N21/00

CPC分类号： G01N29/022 ,  B82Y30/00 ,  B82Y35/00 ,  G01N29/036 ,  G01N29/326 ,  G01N2291/0215 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0258 ,  G01N2291/0426 ,  G01N2291/0427 ,  Y10T436/143333

摘要： Disclosed are system and method for measuring a bio-element capable of accurately detecting whether a bio-element such as protein, gene and the like is present in an atmosphere or vapor phase having controlled temperature and humidity thereof and measuring a content of the bio-element. According to an embodiment of the invention, the method comprises steps of preparing a cantilever sensor having a plurality of cantilevers; measuring a basis resonant frequency for the plurality of cantilevers; reacting the cantilevers with a sample including a bio-element; measuring resonant frequencies of the cantilevers after the reaction, in a closed system that is isolated from an exterior environment and temperature and humidity thereof are controlled to a specific state; and calculating variations of the resonant frequencies of the cantilevers before and after the reaction to carry out a quantitative analysis of the bio-element included in the sample.

摘要翻译： 本发明公开了一种用于测量生物元素的系统和方法，其能够准确检测在具有受控温度和湿度的气氛或气相中是否存在诸如蛋白质，基因等的生物元件，并测量生物元素的含量， 元件。 根据本发明的实施例，该方法包括制备具有多个悬臂的悬臂传感器的步骤; 测量所述多个悬臂的基本共振频率; 使悬臂与包括生物元件的样品反应; 在与外部环境隔离的封闭系统中测量反应后的悬臂的谐振频率，并将其温度和湿度控制在特定状态; 并计算反应前后悬臂的共振频率的变化，以对样品中包含的生物元素进行定量分析。

公开(公告)号：US20110212511A1

公开(公告)日：2011-09-01

申请号：US13128149

申请日：2008-11-11

申请人： Kyo Seon Hwang ,  Tae Song Kim ,  Sang Kyung Kim ,  Sang Myung Lee ,  Yoon Sik Lee

发明人： Kyo Seon Hwang ,  Tae Song Kim ,  Sang Kyung Kim ,  Sang Myung Lee ,  Yoon Sik Lee

IPC分类号： G01N33/566

CPC分类号： G01N33/54373

摘要： Provided is a protein detection system using a micro-cantilever and based on immune responses, wherein the micro-cantilever shows significantly improved sensitivity to allow detection of a trace amount of biomolecule. To the micro-cantilever, sandwich immunoassay is applied, and the sandwich immunoassay uses a polyclonal antibody or silica nanoparticles having a monoclonal antibody bound thereto, so that variations in the output signals of the cantilever are amplified and the detection sensitivity is significantly improved. The system enables detection of disease specific antigen at several femtomolar levels, and makes it possible to detect a trace amount of protein related to diseases, particularly to cancers, with ease.

摘要翻译： 提供了使用微悬臂梁并基于免疫应答的蛋白质检测系统，其中微悬臂梁显示显着改善的灵敏度以允许检测微量的生物分子。 对微悬臂梁进行夹心免疫测定，夹心免疫测定使用结合有单克隆抗体的多克隆抗体或二氧化硅纳米粒子，扩大悬臂输出信号的变化，检测灵敏度明显提高。 该系统能够以几个摩尔体积水平检测疾病特异性抗原，并且可以容易地检测与疾病，特别是癌症相关的痕量的蛋白质。