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9 条记录 (耗时 0.031 秒)

    GABA transporter protein and DNA thereof
    5.
    发明授权
    GABA transporter protein and DNA thereof 失效
    GABA转运蛋白及其DNA

    公开(公告)号：US06906177B1

    公开(公告)日：2005-06-14

    申请号：US10009693

    申请日：2000-06-08

    申请人： Hiroyuki Kimura ,  Junichi Sakamoto ,  Hidekazu Sawada

    发明人： Hiroyuki Kimura ,  Junichi Sakamoto ,  Hidekazu Sawada

    IPC分类号： A61K38/00 ,  A61P25/00 ,  A61P25/28 ,  C07K14/47 ,  C12N15/12 ,  C07K14/00 ,  C12P21/00

    CPC分类号： C07K14/47 ,  A61K38/00

    摘要： The present invention provides a novel protein and its use. The protein or its salt, the partial protein, its amide or ester, or salts, and DNA encoding the same can be employed for obtaining antibodies and antisera, constructing the expression system of the protein of the present invention, for constructing the assay system for the GABA transporter activity and screen medicament candidate compounds using the expression system, for making drug design based on the steric structure of GABA transporters, as agents for producing probes or PCR primers in gene diagnosis, for preparing transgenic animals or as pharmaceuticals for the prevention/treatment of genetic disorders.

    摘要翻译： 本发明提供了一种新型蛋白质及其用途。 可以使用蛋白质或其盐，部分蛋白质，其酰胺或酯或盐，以及编码该蛋白质或其盐的DNA，以获得抗体和抗血清，构建本发明的蛋白质的表达系统，以构建用于 使用表达系统的GABA转运蛋白活性和筛选药物候选化合物，用于基于GABA转运蛋白的空间结构进行药物设计，作为用于在基因诊断中产生探针或PCR引物的试剂，用于制备转基因动物或作为预防/ 治疗遗传疾病。

    Sodium bicarbonate cotransporter protein
    6.
    发明授权
    Sodium bicarbonate cotransporter protein 失效
    碳酸氢钠协同转运蛋白

    公开(公告)号：US06723522B1

    公开(公告)日：2004-04-20

    申请号：US09868572

    申请日：2001-06-20

    申请人： Hiroyuki Kimura ,  Tomohiro Kawamoto ,  Hidekazu Sawada

    发明人： Hiroyuki Kimura ,  Tomohiro Kawamoto ,  Hidekazu Sawada

    IPC分类号： C07N1400

    CPC分类号： C07K14/47 ,  A61K38/00

    摘要： The present invention relates to a protein having a Na+—HCO3− cotransporter activity and a DNA encoding it. The present protein and the present DNA can be used as or for [1] obtaining an antibody and antiserum, [2] constructing an expression system for the present protein, [3] development of a system for measuring the activity of a Na+—HCO3− cotransporter and screening of a drug candidate compound using the same expression system, [4] performing drug design based on the steric structure of a Na+—HCO3− cotransporter protein, [5] a reagent for making a probe or a PCR primer in gene diagnosis, [6] making a transgenic animal, or [7] a composition for gene prevention and/or treatment.

    摘要翻译： 本发明涉及具有Na + HCO 3 - 共转运蛋白活性的蛋白质和编码其的DNA。本发明的蛋白质和本发明的DNA可用作[1]获得抗体和抗血清[2 ]构建本发明蛋白质的表达系统，[3]开发用于测量Na + -HCO 3 - 共转运蛋白活性的系统，并使用相同的表达系统筛选候选药物[4] 基于Na + - HCO 3 - 共转运蛋白的空间结构的药物设计，[5]用于在基因诊断中制备探针或PCR引物的试剂，[6]制备转基因动物，或[7] 用于基因预防和/或治疗的组合物。

    CHO cells that express human LH-RH receptor
    8.
    发明授权
    CHO cells that express human LH-RH receptor 失效
    表达人LH-RH受体的CHO细胞

    公开(公告)号：US5677184A

    公开(公告)日：1997-10-14

    申请号：US423691

    申请日：1995-04-18

    申请人： Haruo Onda ,  Shoichi Ohkubo ,  Shuji Hinuma ,  Hidekazu Sawada

    发明人： Haruo Onda ,  Shoichi Ohkubo ,  Shuji Hinuma ,  Hidekazu Sawada

    IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K7/23 ,  C07K14/72 ,  C12N15/12 ,  G01N33/53 ,  C12N15/85

    CPC分类号： C07K7/23 ,  C07K14/723 ,  A61K38/00 ,  A61K39/00

    摘要： Disclosed are CHO cells which are capable of continued production of human LH-RH receptor proteins, or cell membrane fractions thereof; recombinant human LH-RH receptor proteins or partial peptides thereof; methods for screening compounds which have affinity for an LH-RH receptor by contacting the compound with the CHO cells or the cell membrane fractions thereof, or the recombinant human LH-RH receptor proteins or the partial peptides thereof; kits for screening them; the compounds which have affinity for the LH-RH receptor obtained by methods for the screening or kits for the screening; and pharmaceutical compositions containing the compound, thereby being able to early provide prophylactic or therapeutic compositions, for example, for prostate cancer, uterine cancer, breast cancer, a pituitary tumor, endometriosis, hysteromyoma or precocious puberty. They are also useful as a pregnancy controlling composition such as contraceptive or a menstrual cycle controlling composition.

    摘要翻译： 公开了能够连续产生人LH-RH受体蛋白质或其细胞膜级分的CHO细胞; 重组人LH-RH受体蛋白或其部分肽; 通过使化合物与CHO细胞或其细胞膜级分或重组人LH-RH受体蛋白或其部分肽接触来筛选对LH-RH受体具有亲和力的化合物的方法; 用于筛选它们的试剂盒 通过筛选方法获得的对LH-RH受体具有亲和力的化合物或用于筛选的试剂盒; 以及含有该化合物的药物组合物，从而能够早期提供例如前列腺癌，子宫癌，乳腺癌，垂体瘤，子宫内膜异位症，子宫肌瘤或早熟青春期的预防或治疗组合物。 它们也可用作妊娠控制组合物，例如避孕或月经周期控制组合物。

    Fibroblast growth factor gene transformed hybridoma and enhanced production of antibody
    9.
    发明授权
    Fibroblast growth factor gene transformed hybridoma and enhanced production of antibody 失效
    纤维蛋白原生长因子基因改造杂交瘤和增强抗体生产

    公开(公告)号：US5213980A

    公开(公告)日：1993-05-25

    申请号：US706946

    申请日：1991-05-29

    申请人： Hidekazu Sawada ,  Keiji Iwamoto ,  Kazuaki Kitano

    发明人： Hidekazu Sawada ,  Keiji Iwamoto ,  Kazuaki Kitano

    IPC分类号： C12N15/09 ,  C07K14/50 ,  C12N5/22 ,  C12P21/00 ,  C12P21/08 ,  C12R1/91

    CPC分类号： C07K14/503 ,  C07K14/501

    摘要： Disclosed are (1) a hybridoma carrying a vector for expressing a fibroblast growth factor (FGF) protein gene; (2) a method for producing the hybridoma of (1) which comprises transforming a hybridoma with the vector for expressing the FGF protein gene; and (3) a method for producing a biologically active substance which comprises cultivating in a culture medium the hybridoma obtained by the method of (2) using a hybridoma producing a biologically active substance other than the FGF protein, producing the FGF protein and producing and accumulating the biologically active substance in a culture, and recovering the biologically active substance, whereby the biologically active substance can be efficiently produced and recovered using the serum-free medium, which is advantageous for industrial production and very useful for an improvement in the breeding of the hybridoma.