-
公开(公告)号：US6063904A
公开(公告)日：2000-05-16
申请号：US127829
申请日：1998-08-03
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222 , C12N2770/36122
摘要： A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.
摘要翻译： 一种细胞外产生丙型肝炎病毒的蛋白质的方法包括以下步骤：培养转化体，该转化体用含有编码丙型肝炎病毒蛋白的DNA片段的表达载体转化，并回收由转化体细胞外产生的丙型肝炎病毒的蛋白质 。 起源于通过该方法制备的E1区域的蛋白质可以用作制备用于预防HCV感染的疫苗的材料。 此外，含有蛋白质的诊断剂可用于HCV抗体的检测或其在血清等中的确认。 换句话说，本发明的蛋白质能够以高特异性和灵敏度诊断C型肝炎。
-
公开(公告)号：US5830691A
公开(公告)日：1998-11-03
申请号：US636883
申请日：1996-04-24
IPC分类号： C12N15/09 , A61K39/00 , C07K14/18 , C12N15/51 , C12P21/02 , C12R1/91 , C12P21/06 , C07H19/00 , C07K1/00 , C12N5/00
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222 , C12N2770/36122
摘要： A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.
摘要翻译： 一种细胞外产生丙型肝炎病毒的蛋白质的方法包括以下步骤：培养转化体，该转化体用含有编码丙型肝炎病毒蛋白的DNA片段的表达载体转化，并回收由转化体细胞外产生的丙型肝炎病毒的蛋白质 。 起源于通过该方法制备的E1区域的蛋白质可以用作制备用于预防HCV感染的疫苗的材料。 此外，含有蛋白质的诊断剂可用于HCV抗体的检测或其在血清等中的确认。 换句话说，本发明的蛋白质能够以高特异性和灵敏度诊断C型肝炎。
-
公开(公告)号：US5789544A
公开(公告)日：1998-08-04
申请号：US462195
申请日：1995-06-05
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222 , C12N2770/36122
摘要： A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.
-
公开(公告)号：US5750331A
公开(公告)日：1998-05-12
申请号：US325630
申请日：1994-10-19
申请人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
发明人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
IPC分类号： C07K14/18 , C12N15/09 , G01N33/576
CPC分类号： C07K14/005 , G01N33/5767 , C12N2770/24222 , Y10S436/82
摘要： A diagnostic reagent for hepatitis C, which detects an antibody induced by infection of hepatitis C virus, and comprises the second envelope protein or first non-structural protein which is encoded by the gene of hepatitis C virus and has a sugar chain. This invention also provide a method for detecting an anti-hepatitis C virus antibody. The use of the diagnostic reagent for hepatitis C according to the present invention makes highly sensitive diagnosis of hepatitis C possible.
摘要翻译： 一种丙型肝炎诊断试剂，其检测由丙型肝炎病毒感染引起的抗体，并且包含由丙型肝炎病毒基因编码并具有糖链的第二包膜蛋白或第一非结构蛋白。 本发明还提供了检测抗丙型肝炎病毒抗体的方法。 根据本发明的丙型肝炎诊断试剂的使用使丙型肝炎的高度敏感性诊断成为可能。
-
公开(公告)号：US5747241A
公开(公告)日：1998-05-05
申请号：US460806
申请日：1995-06-02
申请人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
发明人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
IPC分类号： C07K14/18 , C12N15/09 , G01N33/576 , C12P21/02
CPC分类号： C07K14/005 , G01N33/5767 , C12N2770/24222 , Y10S436/82
摘要： A diagnostic reagent for hepatitis C, which detects an antibody induced by infection of hepatitis C virus, comprising the second envelope protein or first non-structural protein which is encoded by the gene of hepatitis C virus and has a sugar chain. This invention also provides a method for detecting an anti-hepatitis C virus antibody. The use of the diagnostic reagent for hepatitis C according to the present invention makes highly sensitive diagnosis of hepatitis C possible.
摘要翻译： 一种用于检测由丙型肝炎病毒感染引起的抗体的丙型肝炎诊断试剂，其包含由丙型肝炎病毒基因编码并具有糖链的第二包膜蛋白或第一非结构蛋白。 本发明还提供了检测抗丙型肝炎病毒抗体的方法。 根据本发明的丙型肝炎诊断试剂的使用使丙型肝炎的高度敏感性诊断成为可能。
-
-
-
-
搜索结果
5 条记录 (耗时 0.026 秒)
