公开(公告)号：US5955336A

公开(公告)日：1999-09-21

申请号：US906029

申请日：1992-06-26

申请人： Tatsuro Shigyo ,  Kohji Sugihara ,  Yuji Takamoto ,  Masachika Takashio ,  Minoru Kamimura ,  Kazumi Yamamoto ,  Yoshio Kojima ,  Toshiro Kikuchi ,  Shigenori Emi

发明人： Tatsuro Shigyo ,  Kohji Sugihara ,  Yuji Takamoto ,  Masachika Takashio ,  Minoru Kamimura ,  Kazumi Yamamoto ,  Yoshio Kojima ,  Toshiro Kikuchi ,  Shigenori Emi

IPC分类号： C12N9/06 ,  C12N15/53

CPC分类号： C12N9/0046

摘要： This invention relates to DNA containing a gene encoding uricase, a plasmid having said DNA, a transformant containing said plasmid, and a process for producing uricase by using said transformant.

摘要翻译： 本发明涉及含有编码尿酸酶的基因的DNA，具有所述DNA的质粒，含有所述质粒的转化体，以及使用所述转化体产生尿酸酶的方法。

公开(公告)号：US5728562A

公开(公告)日：1998-03-17

申请号：US469649

申请日：1995-06-06

申请人： Tatsuro Shigyo ,  Kohji Sugihara ,  Yuji Takamoto ,  Masachika Takashio ,  Minoru Kamimura ,  Kazumi Yamamoto ,  Yoshio Kojima ,  Toshiro Kikuchi ,  Shigenori Emi

发明人： Tatsuro Shigyo ,  Kohji Sugihara ,  Yuji Takamoto ,  Masachika Takashio ,  Minoru Kamimura ,  Kazumi Yamamoto ,  Yoshio Kojima ,  Toshiro Kikuchi ,  Shigenori Emi

IPC分类号： C12N9/06 ,  C12N15/53

CPC分类号： C12N9/0046

摘要： This invention relates to an isolated uricase which is stable in an aqueous solution at a temperature up to 60.degree. C. and at a pH of 8.0 for 10 minutes.

摘要翻译： 本发明涉及一种分离的尿酸酶，其在高达60℃的温度和8.0的pH下在水溶液中稳定10分钟。

公开(公告)号：US5627065A

公开(公告)日：1997-05-06

申请号：US908620

申请日：1992-06-29

申请人： Kazumi Yamamoto ,  Masanori Oka ,  Toshiro Kikuchi ,  Shigenori Emi

发明人： Kazumi Yamamoto ,  Masanori Oka ,  Toshiro Kikuchi ,  Shigenori Emi

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/86 ,  C12N15/55 ,  C12R1/19 ,  C12N9/80 ,  C12N15/70 ,  C12N15/74

CPC分类号： C12N9/86

摘要： A DNA fragment that contains a gene encoding creatinine amidohydrolase is provided. This invention also provides a recombinant vector containing the said DNA fragment, a transformant containing the said vector, and a method for producing creatinine amidohydrolase by the use of the said transformant.

摘要翻译： 提供含有编码肌酸酐酰胺水解酶的基因的DNA片段。 本发明还提供含有所述DNA片段的重组载体，含有所述载体的转化体，以及通过使用所述转化体产生肌酸酐酰胺水解酶的方法。

公开(公告)号：US4965194A

公开(公告)日：1990-10-23

申请号：US87086

申请日：1987-08-19

申请人： Kazumi Yamamoto ,  Toshiro Kikuchi ,  Shigenori Emi

发明人： Kazumi Yamamoto ,  Toshiro Kikuchi ,  Shigenori Emi

IPC分类号： C12N9/02 ,  C12Q1/26

CPC分类号： C12N9/0008 ,  C12Q1/26 ,  C12Y102/03003 ,  Y10S435/81

摘要： A pyruvate oxidase with excellent thermal stability, which is stable at pH 7.0 for 10 minutes at a temperature of up to 45.degree. C., an analytical method using said pyruvate oxidase, and an analytical reagent used for said method.

摘要翻译： 具有优异的热稳定性的丙酮酸氧化酶，其在高达45℃的温度下在pH 7.0下稳定10分钟，使用所述丙酮酸氧化酶的分析方法和用于所述方法的分析试剂。

公开(公告)号：US5795766A

公开(公告)日：1998-08-18

申请号：US611361

申请日：1996-03-06

申请人： Yuzuru Suzuki ,  Yukio Takii ,  Kazumi Yamamoto ,  Yoshiaki Nishiya ,  Atsushi Sogabe ,  Yukihiro Sogabe ,  Shigenori Emi

发明人： Yuzuru Suzuki ,  Yukio Takii ,  Kazumi Yamamoto ,  Yoshiaki Nishiya ,  Atsushi Sogabe ,  Yukihiro Sogabe ,  Shigenori Emi

IPC分类号： C12N9/26 ,  C12N15/56 ,  C12N9/28 ,  C12N1/00 ,  C12N5/10 ,  C12N15/63

CPC分类号： C12N9/2414 ,  C12N9/2408

摘要： A protein having an .alpha.-glucosidase activity, wherein its N-terminal amino acid sequence is the sequence depicted in the Sequence Listing of the present invention at Sequence No. 1; a DNA having the genetic information of the protein having an .alpha.-glucosidase activity; a recombinant vector containing said DNA; a transformant transformed with said vector; and production of .alpha.-glucosidase comprising culture of said transformant in a medium so as to grow .alpha.-glucosidase, and harvesting said .alpha.-glucosidase. According to the present invention, .alpha.-glucosidase without contamination of amylase can be easily produced in high yields and in large amounts by genetic engineering.

摘要翻译： 具有α-葡糖苷酶活性的蛋白质，其N-末端氨基酸序列是序列号1的本发明的序列表中所示的序列; 具有具有α-葡糖苷酶活性的蛋白质的遗传信息的DNA; 含有所述DNA的重组载体; 用所述载体转化的转化体; 和α-葡萄糖苷酶的生产，包括在培养基中培养所述转化体，以便生长α-葡萄糖苷酶，并收获所述α-葡糖苷酶。 根据本发明，通过遗传工程可以容易地以高产率和大量生产不污染淀粉酶的α-葡糖苷酶。

公开(公告)号：US5550046A

公开(公告)日：1996-08-27

申请号：US39777

申请日：1993-03-22

申请人： Yuzuru Suzuki ,  Yukio Takii ,  Kazumi Yamamoto ,  Yoshiaki Nishiya ,  Atsushi Sogabe ,  Yukihiro Sogabe ,  Shigenori Emi

发明人： Yuzuru Suzuki ,  Yukio Takii ,  Kazumi Yamamoto ,  Yoshiaki Nishiya ,  Atsushi Sogabe ,  Yukihiro Sogabe ,  Shigenori Emi

IPC分类号： C12N9/26 ,  C12N15/56 ,  C12N9/28 ,  C12N1/00 ,  C12N5/10 ,  C12N15/63

CPC分类号： C12N9/2414 ,  C12N9/2408

摘要： A protein having an .alpha.-glucosidase activity, wherein its N-terminal amino acid sequence is the sequence depicted in the Sequence Listing of the present invention at Sequence No: 1; a DNA having the genetic information of the protein having an .alpha.-glucosidase activity; a recombinant vector containing said DNA; a transformant transformed with said vector; and production of .alpha.-glucosidase comprising culture of said transformant in a medium so as to grow .alpha.-glucosidase, and harvesting said .alpha.-glucosidase. According to the present invention, .alpha.-glucosidase without contamination of amylase can be easily produced in high yields and in large amounts by genetic engineering.

摘要翻译： 具有α-葡糖苷酶活性的蛋白质，其N-末端氨基酸序列是序列号1中本发明的序列表中所示的序列; 具有具有α-葡糖苷酶活性的蛋白质的遗传信息的DNA; 含有所述DNA的重组载体; 用所述载体转化的转化体; 和α-葡萄糖苷酶的生产，包括在培养基中培养所述转化体，以便生长α-葡萄糖苷酶，并收获所述α-葡糖苷酶。 根据本发明，通过遗传工程可以容易地以高产率和大量生产不污染淀粉酶的α-葡糖苷酶。

公开(公告)号：US5744342A

公开(公告)日：1998-04-28

申请号：US838418

申请日：1997-04-07

申请人： Atsushi Sogabe ,  Seiji Takeshima ,  Kazumi Yamamoto ,  Shinichi Teshima ,  Shigenori Emi ,  Yoshihisa Kawamura

发明人： Atsushi Sogabe ,  Seiji Takeshima ,  Kazumi Yamamoto ,  Shinichi Teshima ,  Shigenori Emi ,  Yoshihisa Kawamura

IPC分类号： C12N9/04 ,  C12N15/53

CPC分类号： C12N9/0006

摘要： A novel protein having a heat-resistant malate dehydrogenase activity, a DNA fragment having a gene encoding said protein, a recombinant vector having said DNA fragment, a transformant transformed with said vector, a method for producing the protein having heat-resistant malate dehydrogenase activity by the use of said transformant, a reagent for GOT determination, comprising the above-mentioned novel protein having a heat-resistant malate dehydrogenase activity and a method for determining GOT activity, which comprises the use of said reagent. According to the present invention, a protein having a heat-resistant malate dehydrogenase activity and having higher purity and superior heat stability can be obtained. In addition, a reagent for GOT determination which is superior in long-term storage can be prepared by the use of the protein having a heat-resistant malate dehydrogenase activity.

摘要翻译： 具有耐热苹果酸脱氢酶活性的新型蛋白质，具有编码所述蛋白质的基因的DNA片段，具有所述DNA片段的重组载体，用该载体转化的转化体，具有耐热性苹果酸脱氢酶活性的蛋白质的制造方法 通过使用所述转化体，用于GOT测定的试剂，其包含上述具有耐热苹果酸脱氢酶活性的新型蛋白质和确定GOT活性的方法，其包括使用所述试剂。 根据本发明，可以得到具有耐热苹果酸脱氢酶活性，纯度高，热稳定性优异的蛋白质。 此外，可以通过使用具有耐热苹果酸脱氢酶活性的蛋白质来制备长期保存优良的GOT测定用试剂。

公开(公告)号：US5686294A

公开(公告)日：1997-11-11

申请号：US270013

申请日：1994-07-01

申请人： Atsushi Sogabe ,  Seiji Takeshima ,  Kazumi Yamamoto ,  Shinichi Teshima ,  Shigenori Emi ,  Yoshihisa Kawamura

发明人： Atsushi Sogabe ,  Seiji Takeshima ,  Kazumi Yamamoto ,  Shinichi Teshima ,  Shigenori Emi ,  Yoshihisa Kawamura

IPC分类号： C12N9/04 ,  C12N15/53

CPC分类号： C12N9/0006

摘要： A novel protein having a heat-resistant malate dehydrogenase activity, a DNA fragment having a gene encoding said protein, a recombinant vector having said DNA fragment, a transformant transformed with said vector, a method for producing the protein having heat-resistant malate dehydrogenase activity by the use of said transformant, a reagent for GOT determination, comprising the above-mentioned novel protein having a heat-resistant malate dehydrogenase activity and a method for determining GOT activity, which comprises the use of said reagent. According to the present invention, a protein having a heat-resistant malate dehydrogenase activity and having higher purity and superior heat stability can be obtained. In addition, a reagent for GOT determination which is superior in long-term storage can be prepared by the use of the protein having a heat-resistant malate dehydrogenase activity.

摘要翻译： 具有耐热苹果酸脱氢酶活性的新型蛋白质，具有编码所述蛋白质的基因的DNA片段，具有所述DNA片段的重组载体，用该载体转化的转化体，具有耐热性苹果酸脱氢酶活性的蛋白质的制造方法 通过使用所述转化体，用于GOT测定的试剂，其包含上述具有耐热苹果酸脱氢酶活性的新型蛋白质和确定GOT活性的方法，其包括使用所述试剂。 根据本发明，可以得到具有耐热苹果酸脱氢酶活性，纯度高，热稳定性优异的蛋白质。 此外，可以通过使用具有耐热苹果酸脱氢酶活性的蛋白质来制备长期保存优良的GOT测定用试剂。

公开(公告)号：US08723054B2

公开(公告)日：2014-05-13

申请号：US12594986

申请日：2008-04-10

申请人： Kazumi Yamamoto ,  Tetsuya Kimura

发明人： Kazumi Yamamoto ,  Tetsuya Kimura

IPC分类号： H05K9/00

CPC分类号： B29C51/02 ,  H01F1/344 ,  H01F1/375 ,  H01F41/16 ,  H01Q17/004 ,  H05K9/0083

摘要： An electromagnetic noise suppression sheet obtained by using combination of a conductive carbon and spinel ferrite particles having a cumulative 50% volume particle diameter of 1 to 10 μm, can exhibit an excellent transmission attenuation power ratio and a reduced return loss in a near electromagnetic field, and is suitable for high-density mounting. The electromagnetic noise suppression sheet of the present invention can be produced by the process of the present invention which includes the steps of applying a coating material in which the conductive carbon and the spinel ferrite particles having a cumulative 50% volume particle diameter of 1 to 10 μm are dispersed, to form a coating film having a thickness of 10 to 100 μm after dried, and subjecting the resulting coating film to thermoforming under pressure.

摘要翻译： 通过使用具有累积的50％体积粒径为1〜10μm的导电性碳和尖晶石型铁氧体颗粒的组合而获得的电磁噪声抑制片可以表现出良好的透射衰减功率比和近电磁场中的降低的回波损耗， 适用于高密度安装。 本发明的电磁噪声抑制片可以通过本发明的方法制造，该方法包括以下步骤：施加具有累积的50％体积粒径的导电性碳和尖晶石型铁素体粒子为1〜10的涂层材料 分散，在干燥后形成厚度为10〜100μm的涂膜，并对所得涂膜进行加压成型。

公开(公告)号：US20130245271A1

公开(公告)日：2013-09-19

申请号：US13885783

申请日：2011-11-16

申请人： Shizuo Shimano ,  Akinori Morikawa ,  Kenichi Yamamoto ,  Hiroki Hotta ,  Kazumi Yamamoto ,  Nozomu Nakanishi ,  Nobuto Minowa

发明人： Shizuo Shimano ,  Akinori Morikawa ,  Kenichi Yamamoto ,  Hiroki Hotta ,  Kazumi Yamamoto ,  Nozomu Nakanishi ,  Nobuto Minowa

IPC分类号： C07D215/22

CPC分类号： C07D215/20 ,  C07D215/14 ,  C07D215/22

摘要： An objective of the present invention is to provide a process for producing 4-carbonyl oxyquinoline derivatives useful as agricultural and horticultural pesticides and fungicides. The objective can be attained by a process for producing 4-carbonyl oxyquinoline derivatives represented by general formula (1), the process including reacting a quinolone derivative with a halogenated compound or an acid anhydride in the presence of a phase transfer catalyst and a base.

摘要翻译： 本发明的目的是提供一种用作农业和园艺农药和杀真菌剂的4-羰基氧喹啉衍生物的方法。 该目的可以通过制备由通式（1）表示的4-羰基氧喹啉衍生物的方法来实现，该方法包括在相转移催化剂和碱的存在下使喹诺酮衍生物与卤代化合物或酸酐反应。