公开(公告)号：US08540864B2

公开(公告)日：2013-09-24

申请号：US12829077

申请日：2010-07-01

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/327

CPC分类号： G01N27/3274 ,  C12Q1/001 ,  C12Q1/006 ,  G01N33/66 ,  G01N33/728

摘要： The present invention provides a method of measuring a component in blood, by which an amount of the component can be corrected accurately by measuring a hematocrit (Hct) value of the blood with high accuracy and high reliability and also provides a sensor used in the method. The sensor for measuring a component in blood has a first analysis portion and a second analysis portion. The first analysis portion has a first electrode system (11,12) and a reagent layer (14), and the reagent layer (14) has an oxidoreductase that acts on the component and a mediator. In the first analysis portion, the component in the blood is measured by causing a redox reaction of the component with the oxidoreductase in the presence of the mediator and detecting a redox current caused when a voltage is applied by the first electrode (11,12). The second analysis portion has a working electrode and a counter electrode, and a mediator is provided on the counter electrode but not on the working electrode. In the second analysis portion, a Hct value of the blood is measured by supplying the blood to the electrode system, applying a voltage to cause a current to flow, and detecting a value of the current. Using this Hct value, the amount of the component is corrected.

摘要翻译： 本发明提供了一种测量血液中的成分的方法，通过以高精度和高可靠性测量血液的血细胞比容（Hct）值，能够精确地校正成分的量，并且还提供了该方法中使用的传感器 。 用于测量血液中成分的传感器具有第一分析部分和第二分析部分。 第一分析部分具有第一电极系统（11,12）和试剂层（14），并且试剂层（14）具有作用于组分的氧化还原酶和介体。 在第一分析部分中，通过在介体存在下引起组分与氧化还原酶的氧化还原反应并检测当通过第一电极（11,12）施加电压时引起的氧化还原电流来测量血液中的组分， 。 第二分析部分具有工作电极和对电极，并且在对电极上而不是在工作电极上设置介体。 在第二分析部分中，通过将血液供给到电极系统，施加电压以使电流流动并检测电流的值来测量血液的Hct值。 使用此Hct值，校正组件的数量。

公开(公告)号：US20070062822A1

公开(公告)日：2007-03-22

申请号：US10578988

申请日：2004-12-03

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01F1/64 ,  G01N33/487

CPC分类号： G01N27/3274

摘要： The present invention provides a method of electrochemically measuring a hematocrit (Hct) value using a sensor, capable of achieving excellent measurement accuracy and reliability and also provides a sensor used in the method. The method of electrochemically measuring a hematocrit (Hct) value of blood include: providing an electrode system having a working electrode (11) and a counter electrode (12), in which a redox substance is provided on the counter electrode (12) but not on the working electrode (11); supplying blood to the electrode system; applying a voltage to the electrode system in this state to cause an oxidation current or a reduction current to flow between the working electrode (11) and the counter electrode (12); detecting the oxidation current or the reduction current; and determining a Hct value based on a value of the detected current.

摘要翻译： 本发明提供一种使用传感器电化学测量血细胞比容（Hct）值的方法，其能够实现优异的测量精度和可靠性，并且还提供了该方法中使用的传感器。 电化学测量血液血细胞比容（Hct）值的方法包括：提供具有工作电极（11）和对电极（12）的电极系统，其中在对电极（12）上设置氧化还原物质 在工作电极（11）上; 向电极系统供应血液; 在该状态下向电极系统施加电压，使得氧化电流或还原电流在工作电极（11）和对电极（12）之间流动。 检测氧化电流或还原电流; 以及基于检测到的电流的值确定Hct值。

公开(公告)号：US08524055B2

公开(公告)日：2013-09-03

申请号：US13094356

申请日：2011-04-26

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/327 ,  G01N33/66

CPC分类号： G01N27/3272 ,  A61B5/14546 ,  A61B5/1486 ,  C12Q1/004 ,  C12Q1/006 ,  G01N27/3274 ,  G01N27/3277 ,  G01N27/413 ,  G01N33/49

摘要： The present invention provides a method of measuring a component in blood, by which the amounts of blood cells and an interfering substance can be measured with high accuracy and high reliability and the amount of the component can be corrected accurately based on the amounts of the blood cells and the interfering substance. In a sensor for measuring a blood component, a first working electrode 13 measures a current that flows during a redox reaction of a blood component, a second working electrode 17 measures the amount of blood cells, and a third working electrode 12 measures the amount of an interfering substance. Next, based on the measurement results, the amount of the blood component to be measured is corrected. Thus, more accurate and precise measurement of the amount of the blood component can be realized.

摘要翻译： 本发明提供一种测定血液成分的方法，通过该方法能够以高精度，高可靠性测定血细胞量和干扰物质的量，并且能够根据血液的量准确地校正成分的量 细胞和干扰物质。 在用于测量血液成分的传感器中，第一工作电极13测量在血液成分的氧化还原反应期间流动的电流，第二工作电极17测量血细胞的量，第三工作电极12测量 干扰物质。 接下来，基于测定结果，校正被测量的血液成分的量。 因此，可以实现对血液成分量的更准确和精确的测量。

公开(公告)号：US07955492B2

公开(公告)日：2011-06-07

申请号：US10598001

申请日：2005-04-18

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/26 ,  G01N27/327

CPC分类号： G01N27/3272 ,  A61B5/14546 ,  A61B5/1486 ,  C12Q1/004 ,  C12Q1/006 ,  G01N27/3274 ,  G01N27/3277 ,  G01N27/413 ,  G01N33/49

摘要： The present invention provides a method of measuring a component in blood, by which the amounts of blood cells and an interfering substance can be measured with high accuracy and high reliability and the amount of the component can be corrected accurately based on the amounts of the blood cells and the interfering substance. In a sensor for measuring a blood component, a first working electrode 13 measures a current that flows during a redox reaction of a blood component, a second working electrode 17 measures the amount of blood cells, and a third working electrode 12 measures the amount of an interfering substance. Next, based on the measurement results, the amount of the blood component to be measured is corrected. Thus, more accurate and precise measurement of the amount of the blood component can be realized.

摘要翻译： 本发明提供一种测定血液成分的方法，通过该方法能够以高精度，高可靠性测定血细胞量和干扰物质的量，并且能够根据血液的量准确地校正成分的量 细胞和干扰物质。 在用于测量血液成分的传感器中，第一工作电极13测量在血液成分的氧化还原反应期间流动的电流，第二工作电极17测量血细胞的量，第三工作电极12测量 干扰物质。 接下来，基于测定结果，校正被测量的血液成分的量。 因此，可以实现对血液成分量的更准确和精确的测量。

公开(公告)号：US08535497B2

公开(公告)日：2013-09-17

申请号：US10578275

申请日：2004-12-03

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/327

CPC分类号： G01N27/3274 ,  C12Q1/001 ,  C12Q1/006 ,  G01N33/66 ,  G01N33/728

摘要： The present invention provides a method of measuring a component in blood, by which an amount of the component can be corrected accurately by measuring a hematocrit (Hct) value of the blood with high accuracy and high reliability and also provides a sensor used in the method. The sensor for measuring a component in blood has a first analysis portion and a second analysis portion. The first analysis portion has a first electrode system (11,12) and a reagent layer (14), and the reagent layer (14) has an oxidoreductase that acts on the component and a mediator. In the first analysis portion, the component in the blood is measured by causing a redox reaction of the component with the oxidoreductase in the presence of the mediator and detecting a redox current caused when a voltage is applied by the first electrode (11,12). The second analysis portion has a working electrode and a counter electrode, and a mediator is provided on the counter electrode but not on the working electrode. In the second analysis portion, a Hct value of the blood is measured by supplying the blood to the electrode system, applying a voltage to cause a current to flow, and detecting a value of the current. Using this Hct value, the amount of the component is corrected.

公开(公告)号：US08088271B2

公开(公告)日：2012-01-03

申请号：US10578988

申请日：2004-12-03

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/26 ,  G01N33/487

CPC分类号： G01N27/3274

摘要： The present invention provides a method of electrochemically measuring a hematocrit (Hct) value using a sensor, capable of achieving excellent measurement accuracy and reliability and also provides a sensor used in the method. The method of electrochemically measuring a hematocrit (Hct) value of blood include: providing an electrode system having a working electrode (11) and a counter electrode (12), in which a redox substance is provided on the counter electrode (12) but not on the working electrode (11); supplying blood to the electrode system; applying a voltage to the electrode system in this state to cause an oxidation current or a reduction current to flow between the working electrode (11) and the counter electrode (12); detecting the oxidation current or the reduction current; and determining a Hct value based on a value of the detected current.

摘要翻译： 本发明提供一种使用传感器电化学测量血细胞比容（Hct）值的方法，其能够实现优异的测量精度和可靠性，并且还提供了该方法中使用的传感器。 电化学测量血液血细胞比容（Hct）值的方法包括：提供具有工作电极（11）和对电极（12）的电极系统，其中在对电极（12）上设置氧化还原物质 在工作电极（11）上; 向电极系统供应血液; 在该状态下向电极系统施加电压，使得氧化电流或还原电流在工作电极（11）和对电极（12）之间流动。 检测氧化电流或还原电流; 以及基于检测到的电流的值确定Hct值。

公开(公告)号：US07641785B2

公开(公告)日：2010-01-05

申请号：US10954020

申请日：2004-09-28

申请人： Teppei Shinno ,  Shin Ikeda

发明人： Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/327

CPC分类号： C12Q1/001 ,  C12Q1/006 ,  G01N27/3274 ,  G01N33/66 ,  G01N33/721

摘要： A sensor for blood component analysis that can correct the effect of a hematocrit easily is provided. The sensor includes an analysis portion including a working electrode, a counter electrode, and a reagent portion. The reagent portion includes an oxidoreductase that reacts with the blood component and a mediator, and the blood component is measured by causing a redox reaction between the blood component and the oxidoreductase in the presence of the mediator and detecting a redox current generated by the redox reaction by the working electrode and the counter electrode. In this sensor, the reagent portion further includes a hemolyzing agent (e.g., sodium cholate) for hemolyzing an erythrocyte, and when detecting the redox current, the erythrocyte is hemolyzed with the hemolyzing agent so as to cause hemoglobin released to an outside of the erythrocyte to react with the mediator and a current generated by this reaction also is detected to correct an effect of a hematocrit.

摘要翻译： 提供了可以容易地校正血细胞比容的影响的用于血液成分分析的传感器。 传感器包括分析部分，其包括工作电极，对电极和试剂部分。 试剂部分包括与血液成分和介体反应的氧化还原酶，并且通过在介体存在下引起血液成分和氧化还原酶之间的氧化还原反应来测定血液成分，并检测由氧化还原反应产生的氧化还原电流 通过工作电极和对电极。 在该传感器中，试剂部还含有用于溶血红细胞的溶血剂（例如胆酸钠），当检测到氧化还原电流时，红细胞用溶血剂溶血，使血红蛋白释放到红细胞外 与介体反应，并且检测由该反应产生的电流以校正血细胞比容的影响。

公开(公告)号：US08480869B2

公开(公告)日：2013-07-09

申请号：US13279787

申请日：2011-10-24

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/327

CPC分类号： G01N27/3274

摘要： The present invention provides a method of electrochemically measuring a hematocrit (Hct) value using a sensor, capable of achieving excellent measurement accuracy and reliability and also provides a sensor used in the method. The method of electrochemically measuring a hematocrit (Hct) value of blood include: providing an electrode system having a working electrode (11) and a counter electrode (12), in which a redox substance is provided on the counter electrode (12) but not on the working electrode (11); supplying blood to the electrode system; applying a voltage to the electrode system in this state to cause an oxidation current or a reduction current to flow between the working electrode (11) and the counter electrode (12); detecting the oxidation current or the reduction current; and determining a Hct value based on a value of the detected current.

摘要翻译： 本发明提供一种使用传感器电化学测量血细胞比容（Hct）值的方法，其能够实现优异的测量精度和可靠性，并且还提供了该方法中使用的传感器。 电化学测量血液血细胞比容（Hct）值的方法包括：提供具有工作电极（11）和对电极（12）的电极系统，其中在对电极（12）上设置氧化还原物质 在工作电极（11）上; 向电极系统供应血液; 在该状态下向电极系统施加电压，使得氧化电流或还原电流在工作电极（11）和对电极（12）之间流动。 检测氧化电流或还原电流; 以及基于检测到的电流的值确定Hct值。

公开(公告)号：US20120037506A1

公开(公告)日：2012-02-16

申请号：US13279787

申请日：2011-10-24

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/327 ,  G01N33/50

CPC分类号： G01N27/3274

摘要： The present invention provides a method of electrochemically measuring a hematocrit (Hct) value using a sensor, capable of achieving excellent measurement accuracy and reliability and also provides a sensor used in the method. The method of electrochemically measuring a hematocrit (Hct) value of blood include: providing an electrode system having a working electrode (11) and a counter electrode (12), in which a redox substance is provided on the counter electrode (12) but not on the working electrode (11); supplying blood to the electrode system; applying a voltage to the electrode system in this state to cause an oxidation current or a reduction current to flow between the working electrode (11) and the counter electrode (12); detecting the oxidation current or the reduction current; and determining a Hct value based on a value of the detected current.

摘要翻译： 本发明提供一种使用传感器电化学测量血细胞比容（Hct）值的方法，其能够实现优异的测量精度和可靠性，并且还提供了该方法中使用的传感器。 电化学测量血液血细胞比容（Hct）值的方法包括：提供具有工作电极（11）和对电极（12）的电极系统，其中在对电极（12）上设置氧化还原物质 在工作电极（11）上; 向电极系统供应血液; 在该状态下向电极系统施加电压，使得氧化电流或还原电流在工作电极（11）和对电极（12）之间流动。 检测氧化电流或还原电流; 以及基于检测到的电流的值确定Hct值。

公开(公告)号：US20070138026A1

公开(公告)日：2007-06-21

申请号：US10598001

申请日：2005-04-18

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： C12Q1/00 ,  G01N33/487

CPC分类号： G01N27/3272 ,  A61B5/14546 ,  A61B5/1486 ,  C12Q1/004 ,  C12Q1/006 ,  G01N27/3274 ,  G01N27/3277 ,  G01N27/413 ,  G01N33/49

摘要： The present invention provides a method of measuring a component in blood, by which the amounts of blood cells and an interfering substance can be measured with high accuracy and high reliability and the amount of the component can be corrected accurately based on the amounts of the blood cells and the interfering substance. In a sensor for measuring a blood component, a first working electrode 13 measures a current that flows during a redox reaction of a blood component, a second working electrode 17 measures the amount of blood cells, and a third working electrode 12 measures the amount of an interfering substance. Next, based on the measurement results, the amount of the blood component to be measured is corrected. Thus, more accurate and precise measurement of the amount of the blood component can be realized.

摘要翻译： 本发明提供一种测定血液成分的方法，通过该方法能够以高精度，高可靠性测定血细胞量和干扰物质的量，并且能够根据血液的量准确地校正成分的量 细胞和干扰物质。 在用于测量血液成分的传感器中，第一工作电极13测量在血液成分的氧化还原反应期间流动的电流，第二工作电极17测量血细胞的量，第三工作电极12测量 干扰物质。 接下来，基于测定结果，校正被测量的血液成分的量。 因此，可以实现对血液成分量的更准确和精确的测量。