公开(公告)号：US06692958B2

公开(公告)日：2004-02-17

申请号：US10003539

申请日：2001-12-04

申请人： Teruaki Sekine ,  Kiminari Ito ,  Norio Shimizu ,  Kenzo Bamba ,  Tomohiro Yamaguchi ,  Yasuyuki Kuroiwa

发明人： Teruaki Sekine ,  Kiminari Ito ,  Norio Shimizu ,  Kenzo Bamba ,  Tomohiro Yamaguchi ,  Yasuyuki Kuroiwa

IPC分类号： C12N508

CPC分类号： C12N5/0636 ,  A61K2039/5158 ,  C12N2501/23 ,  C12N2501/515

摘要： Activated lymphocytes derived from cord blood are excellently effective for preventing and treating various types of tumors and various types of infection. With interleukin 2 and/or anti-CD3 antibody, the lymphocytes derived from the cord blood is prepared by segregating lymphocytes from the cord blood and proliferating the segregated lymphocytes directly in vitro or segregating monocytes from cord blood and proliferating the monocytes in vitro. Also, the cord blood-derived activated lymphocytes can be effectively used for preventing recurrence of the diseases and promoting the take of stem cells or other organs.

摘要翻译： 来自脐带血的活化淋巴细胞对于预防和治疗各种类型的肿瘤和各种​​感染是非常有效的。 通过白细胞介素2和/或抗CD3抗体，通过从脐带血分离淋巴细胞并且在体外直接增殖分离的淋巴细胞或从体外将脐带血分离单核细胞并增殖单核细胞来制备来自脐带血的淋巴细胞。 此外，脐带血衍生的活化淋巴细胞可以有效地用于预防疾病的复发和促进干细胞或其他器官的摄入。

公开(公告)号：US06544514B2

公开(公告)日：2003-04-08

申请号：US09288234

申请日：1999-04-08

申请人： Teruaki Sekine ,  Kiminari Ito ,  Kenzo Bamba ,  Yasuyuki Kuroiwa

发明人： Teruaki Sekine ,  Kiminari Ito ,  Kenzo Bamba ,  Yasuyuki Kuroiwa

IPC分类号： C12N500

CPC分类号： C12N5/0636 ,  A61K2035/124 ,  C12N2501/23 ,  C12N2501/515

摘要： Compositions or medicaments comprising allogenic activated-CD4+ cells as the main ingredient, which are attributable to an organ or bone marrow transplantation donor, have an extremely beneficial effect for preventing recidivation of a tumor and remedying various infections such as viral infections, and autoimmune diseases such as phagocytosis, without causing any serious GVHD. The allogenic activated-CD4+ cells can be produced by separating lymphocyte cells or CD4+ cells from peripheral blood of the donor or removing CD8+ cells from the peripheral blood of the donor by using anti-CD8+ antibodies to obtain CD4+ cells and proliferating the CD4+ cells thus prepared in proliferation activator.

摘要翻译： 可归因于器官或骨髓移植供体的包含同种异体活化的CD4 +细胞作为主要成分的组合物或药物对于预防肿瘤的再活化和修复各种感染例如病毒感染和自身免疫疾病具有非常有益的作用，例如 作为吞噬作用，不引起任何严重的GVHD。 通过使用抗CD8 +抗体从供体的外周血中分离淋巴细胞或CD4 +细胞或从供体的外周血中分离出CD8 +细胞，可以产生同种异体活化的CD4 +细胞，从而获得CD4 +细胞并增殖如此制备的CD4 +细胞 在增殖激活剂中。

公开(公告)号：US20080014174A1

公开(公告)日：2008-01-17

申请号：US11834342

申请日：2007-08-06

申请人： Yasuyuki KUROIWA ,  Tomohiro Morio ,  Norio Shimizu ,  Teruaki Sekine

发明人： Yasuyuki KUROIWA ,  Tomohiro Morio ,  Norio Shimizu ,  Teruaki Sekine

IPC分类号： A61K35/14 ,  A61K38/20 ,  A61K39/395 ,  A61P31/00 ,  A61P35/00 ,  A61P37/00

CPC分类号： C12N5/0636 ,  A61K2035/124 ,  C12N2501/23 ,  C12N2501/515

摘要： According to the present invention, HLA matching donor-originating activated lymphocytes highly effective in the treatment of patients with tumors, viral infections and autoimmune diseases are prepared by collecting cells contained in peripheral blood originating from an HLA matching donor or peripheral blood originating from an HLA matching donor achieving micro-chimerism and by stimulating and propagating the cells thus collected with, for instance, interleukin 2 and anti-CD3 antibodies or the like. The HLA matching donor-originating activated lymphocytes thus prepared can be administered to an HLA matching patient with a tumor, a viral infection or an autoimmune disease for therapeutic or preventive purposes.

摘要翻译： 根据本发明，通过收集源自HLA匹配供体的外周血中含有的细胞，来源于HLA的外周血来源的HLA，可以通过收集高度有效治疗肿瘤，病毒感染和自身免疫疾病患者的HLA匹配供体来源的活化淋巴细胞 匹配供体实现微嵌合体，并通过刺激和繁殖用例如白细胞介素2和抗CD3抗体等收集的细胞。 如此制备的HLA匹配供体来源的活化淋巴细胞可以用于具有肿瘤，病毒感染或自身免疫疾病的HLA匹配患者用于治疗或预防目的。

公开(公告)号：US07105155B2

公开(公告)日：2006-09-12

申请号：US09944360

申请日：2001-09-04

申请人： Teruaki Sekine ,  Tadatoshi Takayama

发明人： Teruaki Sekine ,  Tadatoshi Takayama

IPC分类号： A61K35/00

CPC分类号： C12N5/0635 ,  A61K35/17 ,  A61K2035/124 ,  C12N5/0634 ,  C12N5/0636 ,  C12N2501/2302 ,  C12N2501/515

摘要： Activated lymphocytes are administered to a cancer patient at least five or more times within eight months after performing surgical and chemotherapeutic treatment or radiotherapy for treating cancer particularly liver cancer, so that recurrence of the cancer can be prevented over a long period of five or more years. The activated lymphocytes to be administered while performing treatment of cancer may be autologously derived from a cancer patient or collected from the other cancer patient at need. The activated lymphocytes can be cultivated for proliferating or activating lymphocyte cells collected in the presence of solid-phase anti-CD3 antigen and interleukin 2.

公开(公告)号：US4735778A

公开(公告)日：1988-04-05

申请号：US899039

申请日：1986-08-22

申请人： Nobuo Maruyama ,  Yoichi Shibata ,  Teruaki Sekine

发明人： Nobuo Maruyama ,  Yoichi Shibata ,  Teruaki Sekine

IPC分类号： B01L3/00 ,  G01N31/20 ,  G01N35/00

CPC分类号： B01L3/5085 ,  G01N31/20 ,  G01N2035/00297 ,  Y10S436/809 ,  Y10T428/24149

摘要： A microtiter plate is made of a light-transmitting antistatic resin and defines at least one well. The bottom wall of the well is centrally equipped with a flat portion having a diameter of at least 1 mm and is preferably connected continuously to the corresponding peripheral wall by way of a rising part. The minimum radius of curvature of the inner wall of said well, said inner wall including said rising part, is preferably at least 0.5 mm.

摘要翻译： 微量滴定板由透光抗静电树脂制成，并限定至少一个孔。 井的底壁中央配备有直径至少为1mm的平坦部分，并且优选地通过上升部分连续连接到相应的周壁。 所述井的内壁的最小曲率半径，所述内壁包括所述上升部分，优选为至少0.5mm。

公开(公告)号：US4047438A

公开(公告)日：1977-09-13

申请号：US672170

申请日：1976-03-31

申请人： Teruaki Sekine

发明人： Teruaki Sekine

IPC分类号： B01L3/02 ,  G01N35/10

CPC分类号： B01L3/021 ,  B01L2400/0481 ,  G01N35/1065

摘要： Liquid quantitative dispensing apparatus for withdrawing liquid like a syringe into a plurality of pipettes arranged in rows, by simultaneously pressing and releasing cap-like projections formed of flexible material and dispensing the liquid to test tubes and the like.

摘要翻译： 液体定量分配装置，用于通过同时按压和释放由柔性材料形成的帽状突起并将液体分配到试管等中，将诸如注射器的液体排出成多个排成行的移液管。