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1.发明授权
公开(公告)号：US5654180A
公开(公告)日：1997-08-05
申请号：US344683
申请日：1994-11-17
摘要： The present invention provides a recombinant plasmid comprising combining a hybrid plasmid vector with the isolated DNA sequences of one or more genes encoding nitrile degrading enzymes which are derived from bacteria belonging to the genus Rhodococcus, said hybrid plasmid vector comprising an isolated DNA sequence which confers on the vector the ability to replicate and amplify in the cells of bacteria belonging to the genus Rhodococcus, and an isolated DNA sequence which confers on the vector the ability to replicate and amplify in the cells of bacteria belonging to Escherichia coli, and an isolated DNA sequence containing a drug resistance gene.
摘要翻译： 本发明提供了一种重组质粒，其包含将杂交质粒载体与编码来自属于红球菌属的细菌的腈降解酶的一个或多个基因的分离的DNA序列组合，所述杂交质粒载体包含赋予的分离的DNA序列 载体能够在属于红球菌属的细菌的细胞中复制和扩增的能力，以及赋予载体在分离的DNA序列的细胞中复制和扩增能力的分离的DNA序列 含有耐药性基因。
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公开(公告)号：US5731176A
公开(公告)日：1998-03-24
申请号：US28463
申请日：1993-03-09
IPC分类号： C12N15/53 , C07H21/04 , C12N20060101 , C12N1/21 , C12N9/34 , C12N9/78 , C12N15/09 , C12N15/31 , C12N15/55 , C12N15/60 , C12P13/02 , C12R1/01 , C12R1/19 , C12N1/00 , C12N5/00 , C12N15/63
摘要： The present invention has disclosed the amino acid sequence and nucleotide sequence of the .alpha.- and .beta.-subunits of two types of nitrile hydratase derived from Rhodococcus rhodochrous J-1. The DNA fragment encoding nitrile hydratase is inserted into an expression vector and the recombinant vector is used for transformation. The transformant contains multiple copies of the gene and can produce much higher level of nitrile hydratase compared with conventionally used microorganisms.
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3.发明授权Gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant 失效编码具有腈水合酶活性的多肽的基因，含有该基因的转化体和使用转化体生产酰胺的方法
公开(公告)号：US5789211A
公开(公告)日：1998-08-04
申请号：US463470
申请日：1995-06-05
CPC分类号： C12N9/80
摘要： The present invention relates to a gene derived from Pseudomonas chlororaphis B23 strain which encodes a polypeptide having nitrile hydratase activity being capable of hydrating nitriles to amides. The invention also relates to a recombinant DNA containing the gene, and a transformant transformed with the recombinant DNA. The present invention further relates to a method of producing nitrile hydratase using the transformant and of amides using nitrile hydratase.
摘要翻译： 本发明涉及一种源自绿脓假单胞菌B23株的基因，该基因编码具有腈水合酶活性的能够将腈水合至酰胺的多肽。 本发明还涉及含有该基因的重组DNA和用重组DNA转化的转化体。 本发明还涉及使用腈水合酶使用转化体和酰胺生产腈水合酶的方法。
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4.发明授权Gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant 失效编码具有腈水合酶活性的多肽的基因，含有该基因的转化体和使用转化体生产酰胺的方法
公开(公告)号：US5648256A
公开(公告)日：1997-07-15
申请号：US233146
申请日：1994-04-22
CPC分类号： C12N9/80
摘要： The present invention relates to a gene derived from Pseudomonas chlororaphis B23 strain which encodes a polypeptide having nitrile hydratase activity being capable of hydrating nitriles to amides. The invention also relates to a recombinant DNA containing the gene, and a transformant transformed with the recombinant DNA. The present invention further relates to a method of producing nitrile hydratase using the transformant and of amides using nitrile hydratase.
摘要翻译： 本发明涉及一种源自绿脓假单胞菌B23株的基因，该基因编码具有腈水合酶活性的能够将腈水合至酰胺的多肽。 本发明还涉及含有该基因的重组DNA和用重组DNA转化的转化体。 本发明还涉及使用腈水合酶使用转化体和酰胺生产腈水合酶的方法。
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5.发明授权DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant 失效编码具有腈水合酶活性的多肽的DNA片段，含该基因的转化体和使用转化体生产酰胺的方法
公开(公告)号：US5753472A
公开(公告)日：1998-05-19
申请号：US461836
申请日：1995-06-05
CPC分类号： C12P13/02 , C12N9/78 , Y10S435/874
摘要： The present invention has disclosed the amino acid sequence and nucleotide sequence of the .alpha.- and .beta.-subunits of two types of nitrile hydratase derived from Rhodococcus rhodochrous J-1. The DNA fragment encoding nitrile hydratase is inserted into an expression vector and the recombinant vector is used for transformation. The transformant contains multiple copies of the gene and can produce much higher level of nitrile hydratase compared with conventionally used microorganisms.
摘要翻译： 本发明公开了源自玫瑰色红球菌J-1的两种腈水合酶的α-和β-亚基的氨基酸序列和核苷酸序列。 将编码腈水合酶的DNA片段插入表达载体中，并使用重组载体进行转化。 与常规使用的微生物相比，转化体含有多个拷贝的基因，可以产生更高水平的腈水合酶。
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公开(公告)号：US20090009535A1
公开(公告)日：2009-01-08
申请号：US12166659
申请日：2008-07-02
IPC分类号： G09G5/00
CPC分类号： G09G5/14 , G01C21/367 , G06F3/0481 , G06F3/0482 , G06F17/211 , G06F2203/04806 , G09G5/391 , G09G2340/12
摘要： A display processing device for displaying a display object in a display window and changing a display range includes a sectioning unit that divides the display window into a plurality of sections (screens) by creating boundary lines. Maps of geographical ranges to be displayed are sectioned and displayed in the plurality of screens. In the event that a boundary line is moved through a user input, the geographical ranges of the maps in the screens for which the screen size has become larger than an initial screen size are enlarged, and the maps in the screens which have become smaller than the initial screen size are reduced in scale in accordance with the reduction in screen size, while maintaining the geographical range of the maps displayed in the display window prior to the boundary line being moved.
摘要翻译： 一种用于在显示窗口中显示显示对象并改变显示范围的显示处理设备包括：通过创建边界线将显示窗口划分成多个部分（屏幕）的分割单元。 要显示的地理范围的地图被分割并显示在多个屏幕中。 在通过用户输入移动边界线的情况下，屏幕尺寸已经变得大于初始屏幕尺寸的屏幕中的地图的地理范围被放大，并且屏幕中的映射已经变得小于 根据屏幕尺寸的减小，初始屏幕尺寸减小，同时保持在边界线移动之前在显示窗口中显示的地图的地理范围。
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公开(公告)号：USD635970S1
公开(公告)日：2011-04-12
申请号：US29370164
申请日：2010-06-10
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公开(公告)号：US08743150B2
公开(公告)日：2014-06-03
申请号：US12166659
申请日：2008-07-02
IPC分类号： G09G5/00
CPC分类号： G09G5/14 , G01C21/367 , G06F3/0481 , G06F3/0482 , G06F17/211 , G06F2203/04806 , G09G5/391 , G09G2340/12
摘要： A display processing device for displaying a display object in a display window and changing a display range includes a sectioning unit that divides the display window into a plurality of sections (screens) by creating boundary lines. Maps of geographical ranges to be displayed are sectioned and displayed in the plurality of screens. In the event that a boundary line is moved through a user input, the geographical ranges of the maps in the screens for which the screen size has become larger than an initial screen size are enlarged, and the maps in the screens which have become smaller than the initial screen size are reduced in scale in accordance with the reduction in screen size, while maintaining the geographical range of the maps displayed in the display window prior to the boundary line being moved.
摘要翻译： 一种用于在显示窗口中显示显示对象并改变显示范围的显示处理设备包括：通过创建边界线将显示窗口划分为多个部分（屏幕）的分割单元。 要显示的地理范围的地图被分割并显示在多个屏幕中。 在通过用户输入移动边界线的情况下，屏幕尺寸已经变得大于初始屏幕尺寸的屏幕中的地图的地理范围被放大，并且屏幕中的映射已经变得小于 根据屏幕尺寸的减小，初始屏幕尺寸减小，同时保持在边界线移动之前在显示窗口中显示的地图的地理范围。
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