公开(公告)号：US08441633B2

公开(公告)日：2013-05-14

申请号：US12915921

申请日：2010-10-29

申请人： Thai V. Truong ,  John M. Choi ,  Scott E. Fraser ,  Willy Supatto ,  David S. Koos

发明人： Thai V. Truong ,  John M. Choi ,  Scott E. Fraser ,  Willy Supatto ,  David S. Koos

IPC分类号： G01J3/40 ,  G01J3/30 ,  G01J1/58 ,  F21V9/16 ,  G01T1/10 ,  G21H3/02 ,  G21K5/00 ,  H01J65/06 ,  H01J65/08

CPC分类号： G02B21/16 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2021/655 ,  G02B21/002 ,  G02B21/367

摘要： An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal illumination of light sheet microscopy are provided. With live imaging of whole Drosophila and zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in 4D imaging of live biological systems.

摘要翻译： 提供了一种多光子激光荧光与光谱显微镜正交照明相结合的多光子光谱显微镜（MP-LISH）的设备和方法。 通过对整个果蝇和斑马鱼胚胎的实时成像，MP-LISH的高性能与目前最先进的成像技术相比，保持生物组织内部的良好信号和高空间分辨率（比单光子光更深两倍） 片状显微镜），在采集速度（比常规双光子激光扫描显微镜快超过一个数量级），并且具有低的光毒性。 这种新的成像技术的固有多模态也被证明了二次谐波发生光片显微镜检测小鼠尾部组织中的胶原蛋白。 共同的，这些属性为MP-LISH在活体生物系统4D成像中的应用提供了广泛的应用。

公开(公告)号：US20110122488A1

公开(公告)日：2011-05-26

申请号：US12915921

申请日：2010-10-29

申请人： Thai V. Truong ,  John M. Choi ,  Scott E. Fraser ,  Willy Supatto ,  David S. Koos

发明人： Thai V. Truong ,  John M. Choi ,  Scott E. Fraser ,  Willy Supatto ,  David S. Koos

IPC分类号： G02B21/06

CPC分类号： G02B21/16 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2021/655 ,  G02B21/002 ,  G02B21/367

摘要： An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal. illumination of light sheet microscopy are provided. With live imaging of whole Drosophila and zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in 4D imaging of live biological systems.

摘要翻译： 一种多光子激光荧光与正交结合的多光子光谱显微镜（MP-LISH）的设备和方法。 提供了光片显微镜的照明。 通过对整个果蝇和斑马鱼胚胎的实时成像，MP-LISH的高性能与目前最先进的成像技术相比，保持生物组织内部的良好信号和高空间分辨率（比单光子光更深两倍） 片状显微镜），在采集速度（比常规双光子激光扫描显微镜快超过一个数量级），并且具有低的光毒性。 这种新的成像技术的固有多模态也被证明了二次谐波发生光谱显微镜检测小鼠尾部组织中的胶原蛋白。 共同的，这些属性为MP-LISH在活体生物系统4D成像中的应用提供了广泛的应用。

公开(公告)号：US08570649B2

公开(公告)日：2013-10-29

申请号：US12916124

申请日：2010-10-29

申请人： Thai V. Truong ,  John M. Choi ,  Scott E. Fraser ,  Willy Supatto ,  David S. Koos

发明人： Thai V. Truong ,  John M. Choi ,  Scott E. Fraser ,  Willy Supatto ,  David S. Koos

IPC分类号： G02B21/06 ,  G02B21/00

CPC分类号： G02B21/16 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2021/655 ,  G02B21/002 ,  G02B21/367

摘要： An apparatus for and method of performing light sheet microscopy (LISH) and light scanning microscopy (RAPS) in a single device are provided. The dual-mode imaging microscope allows for the use of both LISH and RAPS in a single instrument. This dual-mode device will allow researchers to have access to both types of microscopy, allowing access to the widest possible selection of samples. In addition, the device will reduce the high costs and space requirements associated with owning two different microscopes (LISH and RAPS).

摘要翻译： 提供了在单个装置中执行光谱显微镜（LISH）和光扫描显微镜（RAPS）的装置和方法。 双模式成像显微镜允许在单个仪器中使用LISH和RAPS。 这种双模式设备将使研究人员能够访问这两种类型的显微镜，从而允许访问尽可能多的样品。 此外，该设备将降低与拥有两台不同显微镜（LISH和RAPS）相关的高成本和空间要求。

公开(公告)号：US20110134521A1

公开(公告)日：2011-06-09

申请号：US12916124

申请日：2010-10-29

申请人： Thai V. Truong ,  John M. Choi ,  Scott E. Fraser ,  Willy Supatto ,  David S. Koos

发明人： Thai V. Truong ,  John M. Choi ,  Scott E. Fraser ,  Willy Supatto ,  David S. Koos

IPC分类号： G02B21/06

CPC分类号： G02B21/16 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2021/655 ,  G02B21/002 ,  G02B21/367

摘要： An apparatus for and method of performing light sheet microscopy (LISH) and light scanning microscopy (RAPS) in a single device are provided. The dual-mode imaging microscope allows for the use of both LISH and RAPS in a single instrument. This dual-mode device will allow researchers to have access to both types of microscopy, allowing access to the widest possible selection of samples. In addition, the device will reduce the high costs and space requirements associated with owning two different microscopes (LISH and RAPS).

摘要翻译： 提供了在单个装置中执行光谱显微镜（LISH）和光扫描显微镜（RAPS）的装置和方法。 双模式成像显微镜允许在单个仪器中使用LISH和RAPS。 这种双模式设备将使研究人员能够访问这两种类型的显微镜，从而允许访问尽可能多的样品。 此外，该设备将减少与拥有两台不同显微镜（LISH和RAPS）相关的高成本和空间要求。

公开(公告)号：US08575570B2

公开(公告)日：2013-11-05

申请号：US13217580

申请日：2011-08-25

申请人： John M. Choi ,  Thai V. Truong ,  David S. Koos

发明人： John M. Choi ,  Thai V. Truong ,  David S. Koos

IPC分类号： F21V9/16

CPC分类号： G01N21/4795 ,  G01N21/6458 ,  G02B21/367

摘要： An apparatus for and method of performing orthogonal light sheet microscopy (OLM) and computer optical tomography (COT) simultaneously in a single device are provided. The dual-mode imaging microscope allows for the use of both OLM and COT in a single instrument. This dual-mode device will allow researchers to have access to both types of microscopy, allowing access to the widest possible selection of samples, and improved imaging results. In addition, the device will reduce the high costs and space requirements associated with owning two different imagers (OLM and COT).

摘要翻译： 提供了在单个设备中同时执行正交光谱显微镜（OLM）和计算机光学层析成像（COT）的装置和方法。 双模显微镜允许在单个仪器中使用OLM和COT。 这种双模式设备将允许研究人员访问这两种类型的显微镜，允许访问尽可能多的样品选择和改进的成像结果。 此外，该设备将降低与拥有两个不同成像器（OLM和COT）相关的高成本和空间要求。

公开(公告)号：US20120049087A1

公开(公告)日：2012-03-01

申请号：US13217580

申请日：2011-08-25

申请人： John M. Choi ,  Thai V. Truong ,  David S. Koos

发明人： John M. Choi ,  Thai V. Truong ,  David S. Koos

IPC分类号： G01J1/58 ,  G01J3/44 ,  G01N21/59 ,  G02B21/36

CPC分类号： G01N21/4795 ,  G01N21/6458 ,  G02B21/367

摘要： An apparatus for and method of performing orthogonal light sheet microscopy (OLM) and computer optical tomography (COT) simultaneously in a single device are provided. The dual-mode imaging microscope allows for the use of both OLM and COT in a single instrument. This dual-mode device will allow researchers to have access to both types of microscopy, allowing access to the widest possible selection of samples, and improved imaging results. In addition, the device will reduce the high costs and space requirements associated with owning two different imagers (OLM and COT).

摘要翻译： 提供了在单个设备中同时执行正交光谱显微镜（OLM）和计算机光学层析成像（COT）的装置和方法。 双模显微镜允许在单个仪器中使用OLM和COT。 这种双模式设备将允许研究人员访问这两种类型的显微镜，允许访问尽可能多的样品选择和改进的成像结果。 此外，该设备将降低与拥有两个不同成像器（OLM和COT）相关的高成本和空间要求。